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Biotage® Isolera™ Spektra LS (Large Scale)
Flash Purification System
- TLC-to-Step gradient
- Gradient Optimization
- Real-time PDA scanning
- 2D and 3D spectral analysis
- Optimized for Biotage®
SNAP Ultra cartridges
- λ-All detection
- Baseline correction
- Run counter
- UV-VIS detector
- Flow rates of 50 to 500 mL/min
- Method evaluation and scale-up
- External dry loading vessel
Watch the Isolera Spektra feature demo video
Now even more flexibility and capability
Need to hold a gradient to improve a separation?
It is now possible with a new Isocratic Hold button.
Need to collect a large volume of a single eluting compound?
A new fraction collector bypass feature provides collection of any volume in any clean collection vessel, saving fraction capacity.
Need to multi-task?
Monitor the purification from your office and you can advance fractions, use the Isocratic hold feature, monitor solvent and waste levels, pause the purification run, just to name a few.
Scale from milligrams to >150 gram
on a single system, using a single method
The Biotage Isolera Spektra LS development-scale flash chromatography system dramatically shortens large-scale purification run-times with flow rates of 50–500 mL/minute. Simply select or create a method, load your sample, and run — it’s that easy. New advanced features include: the ability to collect fractions on all wavelengths, use of up to four solvents in a single gradient and add a third isocratic co-solvent. A UV-VIS detector increases detection to 200–800 nm.
Flow rates of 50 to 500 mL/min
Higher flow rates of 50 to 500 mL/min, dramatically shortens purification runs. 1500 g SNAP cartridges purify samples up to 150 g further improving capacity and productivity (Figure 1).
Figure 1. 30 g of a lipophillic oil was purified in only 32 minutes using a SNAP 1500 g cartridge at a flow rate of 500 mL/min.
Method evaluation and scale-up on one system
Develop methods by choosing one of the system’s default gradients, entering your own gradient or using the system’s unique built-in TLC-to-gradient feature. Biotage Isolera Spektra LS has a milligram to >150g purification range. This provides chemists the ability to evaluate a method on a smaller sample prior to scale-up, reducing solvent usage and method development time as shown in Figure 2.
Figure 2. A very lipophilic oil (1 gram load) was first purified on a 50 g Biotage SNAP cartridge using 100% heptane at 50 mL/min (left chromatogram).This oil was then loaded onto a 1500 g Biotage SNAP cartridge (30 gram load)and purified at 500 mL/min under identical solvent conditions (until theend of the run). Full separation is achieved on the larger 1500 g cartridgeeven pulling out early eluting impurities.
Unique sample loading pump
The Biotage Isolera Spektra LS overcomes manual injection issues such as leaks or spills with a built-in peristaltic pump. Made with flexible inert fluoropolymer tubing, this pump enables liquid samples to be efficiently pumped directly into your Biotage SNAP 750 g or SNAP 1500 g flash cartridge.
External dry loading vessel
For samples requiring pre-adsorption onto a solid support, a 500 g capacity dry load vessel accessory is available. The dry load vessel has an adjustable bed for samples as low as 100 g and mounts directly onto the Biotage Isolera Spektra LS saving precious space.
Fraction capacity up to 320 L
The Biotage Isolera Spektra LS has a standard capacity of 9.6 liters. For additional fraction capacity (up to 320 L) a funnel rack kit is available. The funnel rack kit comes with two racks (16 funnels each) and a cart with wheels that holds the system and collection vessels.
Use up to four solvents in a gradient
The Biotage Isolera Spektra LS provides dual-binary gradient capability for purifying samples with diverse polarity ranges. A simple hexane/ethyl acetate gradient becomes a powerful hexane/ethyl acetate to ethyl acetate/methanol gradient without solvent immiscibility or emulsions, providing a more efficient way to purify complex sample mixtures.
Maximize solubility and product recovery
Compound precipitation can cause problems with compound recovery and system overpressure. Biotage Isolera Spektra LS is designed to help alleviate this issue by offering the capability to pump a third solvent isocratically during a binary gradient to maintain compound solubility.
Biotage SNAP XL 750 g and 1500 g cartridges
Biotage SNAP XL 750 g and 1500 g cartridges maximize productivity by providing the highest sample loading capacity, fastest throughput and superior large-scale separations as shown in figure 3.
Figure 3. 80 g (>10% load) of a crude reaction mixture with a TLC ?CV<1 was separated with high resolution on a Biotage SNAP XL 750 g cartridge at 200 mL/min
||Two electric HPFC pumps
||145 psi (10 bar) (up to 450 mL/min),
100 psi (7 bar) at 500 mL/min
||Choice of variable wavelength (200-400 nm) or UV-VIS (200-800 nm) detector
|Flow cell path length
|UV collection modes
||Single/dual/λ-All wavelengths (variable UV and UV-VIS)
||Volume, threshold, threshold with volume, low slope, medium slope, custom slope
||Test tubes (17.5, 18, and 25 mm) and bottles (120 mL, 240 mL, and 480 mL)
||100–240 VAC, 50/60 Hz, 4.0 A
|System control and
|On-board computer with 10.4” diagonal touch screen interface
(W x H x D)
|22.7" x 23.5" x 19.6"
(577 mm x 596 mm x 497 mm)
||30-35 kg (66-77 lb)
|Funnel Rack Kit
(W x H x D)
|37.8" x 41.7" x 26.0"
(960 mm x 1060 mm x 660 mm)
||88 lb (40kg without Isolera or Bottles)
||Biotage Isolera One with sample load pump, variable 200-400nm wavelength detector, & expanded fraction collector bed
||Biotage Isolera One with sample load pump, UV-VIS 200-800 nm wavelength detector, & expanded fraction collector bed
||Isolera Spektra Package
||Isolera Spektra Upgrade
||Dry load vessel kit with holder, one empty cartridge & frit
||Replacement dry load vessels and frits (4/pk)
||Funnel rack kit with cart, two racks, 16 funnels & leak detector
||Biotage SNAP 750 g KP-SIL Cartridges, 2/cs
||Biotage SNAP 1500 g KP-SIL Cartridge 2/cs
||Biotage SNAP 950 g KP-C18-HS Cartridge 1/cs
||Biotage SNAP 1850 g KP-C18-HS Cartridge 1/cs
||Biotage SNAP 900 g KP-NH Cartridge 1/cs
||Biotage SNAP 1800g KP-NH Cartridge 1/cs
||Test tube rack, 18 x 150 mm, 4/pk
||Test tube rack, 25 x 150 mm, 4/pk
||Bottle rack, 120 mL, 4/pk
||Bottle rack, 240 mL, 1/pk
||Bottle rack, 480 mL, 1/pk
||120 mL bottles, 96/cs
||240 mL bottles, 84/cs
||480 mL bottles, 24/cs
||Conductive PTFE Funnel Rack Tubing, 8/pk
||GL 45 caps with Sept for Schott/Corning Bottles, 8/pk
||Female Luer inlet fitting for SNAP 750 g & 1500 g cartridges, 1/pk
||Male Luer outlet fitting for SNAP 750 g & 1500 g cartridges, 1/pk
||Biotage SNAP XL 750 g & 1500 g cartridge holder for Isolera systems, 1/pk