Method Development in Solid Phase Extraction using Non-polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples
This Chemistry Data Sheet includes specific information on the extraction of analytes from aqueous samples (pages 1-3) and a general discussion on the range of ISOLUTE non-polar sorbents (page 4).
Download Technical Note 101 (PDF - 147 K) |
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Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Sorbents for the Extraction of Non-Aqueous Samples
This Chemistry Data Sheet includes specific information on the use of polar ISOLUTE sorbents for the extraction of polar analytes from non-aqueous samples (page 1-2), and a general discussion on polar sorbents (page 3).
Download Technical Note 102 (PDF - 162 K) |
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Method Development in Solid Phase Extraction using ISOLUTE SAX and ISOLUTE PE-AX Columns for the Extraction of Aqueous Samples
This technical notes includes specifics on the use of ISOLUTE SAX and PE-AX for the extraction of anionic analytes from acqueous samples (pages 1-3), ordering information (page 4) and a general discussion on anion exchangers (pages 5-6).
Download Technical Note 103 (PDF - 159 K) |
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Method Development in Solid Phase Extraction using ISOLUTE NH2 SPE columns for the Extraction of Aqueous Samples
This technical note includes specifics on the use of ISOLUTE NH2 for the extraction of anionic analytes from aqueous samples (pages 1-2), order information (page 3) and a general discussion on anion exchangers (pages 4-5).
Download Technical Note 104 (PDF - 141 K) |
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Method Development in Solid Phase Extraction using ISOLUTE PSA SPE Columns for the Extraction of Aqueous Samples
This technical note includes specifics on the use of ISOLUTE PSA for the extraction of anionic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on anion exchangers (pages 4-5).
Download Technical Note 105 (PDF - 148 K) |
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Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX-3 SPE Columns for the Extraction of Aqueous Samples
This technical note includes specifics on the use of ISOLUTE SCX and SCX-3 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchangers (pages 4-5).
Download Technical Note 106 (PDF - 157 K) |
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Method Development in Solid Phase Extraction using ISOLUTE SCX-2 SPE Columns for the Extraction of Aqueous Samples
This technical note includes, specifics on the use of ISOLUTE SCX-2 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchanger sorbents (pages 4-5).
Download Technical Note 107 (PDF - 133 K) |
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Method Development in Solid Phase Extraction using ISOLUTE CBA SPE Columns for the Extraction of Aqueous Samples
This technical note includes specifics on the use of ISOLUTE CBA for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchange sorbents (pages 4-6).
Download Technical Note 108 (PDF - 144 K) |
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Method Development in Solid Phase Extraction using ISOLUTE ENV+ SPE Columns for the Extraction of Aqueous Samples
This technical note includes a general discussion on non-polar sorbents (page 1), specifics on the use of ISOLUTE ENV+ for the extraction of polar analytes from aqueous samples (page 2), and ordering information (page 5).
Download Technical Note 109 (PDF - 155 K) |
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SPE Modes of Operation
A list of the five typical ways of using SPE. Each one offers a solution to different types of sample preparation challenges.
Download Technical Note 110 (PDF - 256 kB) |
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Matrix Solid Phase Dispersion
Matrix Solid Phase Dispersion (MSPD) is a sample preparation technique for use with solid sample matrices. Conventional extraction of organic analytes from tissue usually begins with a homogenization step, followed by tedious liquid-liquid extraction procedures, sample clean-up and trace enrichment. A relatively large amount of sample matrix is required, and solvent consumption is high.
Download Technical Note 111 (PDF - 136 kB) |
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General approach to the Extraction of Basic Drugs from Biological Fluids using Non-polar ISOLUTE SPE Columns
This technical note describes the extraction of basic drugs from biological fluids using non-polar ISOLUTE SPE columns. The extraction of drugs from biological fluids using a purely non-polar retention mechanism can lead to extracts containing a large amount of non-polar co-extracted material, which can interfere with the subsequent analysis.
Download Technical Note 112 (PDF) |
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General Approach to the Extraction of Basic Drugs from Biological
Fluids using ISOLUTE Confirm HCX Mixed Mode SPE Columns
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
Download Technical Note 113 (PDF - 134 kB) |
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Solid Phase Extraction using the 96-well Format ISOLUTE-96 Fixed
Well Plates and ISOLUTE Array 96-well Plates
New approaches to the quantitation of drugs and metabolites in biological fluids have increased the need for high throughput methodologies to avoid the traditional laboratory bottleneck of sample preparation. Solid phase extraction in a 96-well plate format such as ISOLUTE-96 Fixed Well Plates or ISOLUTE Array 96-well Plates is a solution to this problem.
Download Technical Note 114 (PDF - 79 kB) |
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Generic Method for the Extraction of Basic Drugs from Biological
Fluids using ISOLUTE Mixed-mode SPE Columns and 96-well SPE Plates
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Download Technical Note 116 (PDF - 392 kB) |
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The use of ISOLUTE HM-N for rapid sample preparation
ISOLUTE HM-N is a modified form of diatomaceous earth that can efficiently absorb aqueous samples. ISOLUTE HM_N is chemically inert and stable in the pH range 1-13. These characteristics make it a versabile material that plays an important role in many sample preparation applications.
Download Technical Note 118 (PDF - 181 kB) |
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Method Development in Solid Phase Extraction using ISOLUTE 101 SPE Columns for the Extraction of Aqueous Samples
This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3).
Download Technical Note 119 (PDF - 105 kB) |
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Sample Preparation by Mixed-mode SPE using ISOLUTE HCX
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Download Technical Note 125 (PDF) |
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Sample Preparation by Non-polar SPE using ISOLUTE SPE Sorbents
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Download Technical Note 126 (PDF) |
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Sample Preparation by Mixed-mode using ISOLUTE HAX
ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract.
Download Technical Note 127 (PDF) |
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Generic Method for the Extraction of Quaternary Amines and Polybasic Drugs from Biological Fluids using ISOLUTE HCX-Q Mixed-Mode SPE Columns and 96-well Plates
The use of ISOLUTE HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The ISOLUTE HCX-Q sorbent utilizes a combination of weak cation exchange and C8 non-polar retention mechanisms.
Download Technical Note 129 (PDF) |
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Sample Preparation by Protein Precipitation Using the ISOLUTE Protein Precipitation Plate
ISOLUTE PPT+ Protein Precipitation Plates have been designed to overcome the problems of the first generation 96-well filterplates. The functionalized bottom frit holds up organic solvents, in particular acetonitrile, allowing the precipitating solvent to be dispensed into each well prior to sample addition.
Download Technical Note 130 (PDF - 57K) |
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EVOLUTE ABN for Extraction of Drugs from Biological Fluids
EVOLUTE Sample Preparation Products are a new generation of advanced polymeric solid phase extraction sorbents for the high throughput extraction of drugs from biological fluids. Designed to maximize analyte recovery, while reducing the concentration of matrix components in the final extract, EVOLUTE products provide improved extract cleanliness that minimizes ion suppression effects during LC-MS/MS analysis.
Download Technical Note 131 (PDF) |
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EVOLUTE ABN SPE Columns for Solid Phase Extraction of Environmental Samples
EVOLUTE ABN (Acidic Base Neutral) has been developed for the extraction of acidic, neutral or basic compounds from a variety of aqueous matrices. The polystyrene based polymer is surface modified with well defined hydroxyl-functional oligomers, imparting excellent water-wettability. An optimized combination of non-polar (hydrophobic) and polar (hydrophilic) interactions allows efficient extraction of analytes of wide ranging polarities. These characteristics result in a versatile sorbent for extraction of the broad range of analytes encountered in routine environmental analysis.
Download Technical Note 136 (PDF) |
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EVOLUTE ABN Columns for Solid Phase Extraction of Forensic and Clinical Samples
This Chemistry Data Sheet provides guidelines for the simultaneous extraction of acidic, basic and neutral compounds from biological fluids samples using polymer-based non-polar SPE. The generic method is on page 1, with processing and method optimization guidelines on page 2-3. An example application involving the extraction of diuretics from urine illustrates the versatility of EVOLUTE ABN for the extraction of a wide range of analytes (see Appendix).
Download Technical Note TN 137 (PDF) |
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EVOLUTE ABN Columns for Solid Phase Extraction of Aqueous Samples
This Chemistry Data Sheet provides guidelines for the simultaneous extraction of acidic, basic and neutral compounds from aqueous samples or aqueous sample extracts using polymer-based non-polar SPE. The generic method is on page 1, with processing and method optimization guidelines on pages 2-3. An exmaple application showing the trace enrichment of pharmaceuticals in water illustrates the versatility of EVOLUTE ABN for the extraction of a wide range of analytes (see Appendix) from aqueous samples.
Download Technical Note 138 (PDF)
EVOLUTE® CX Columns for Solid Phase Extraction
in Bioanalysis
This Chemistry Data Sheet provides guidelines for the extraction of basic drugs from biological fluid samples using
resin-based mixed-mode SPE.
Download Technical Note 139 (PDF) |
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ISOLUTE SLE+ Supported Liquid Extraction Plates for the Extraction of Drugs from Biological Fluids
ISOLUTE SLE+ 200 and 400 mg Supported Liquid Extraction Plates contain a modified form of diatomaceous earth. The support provides an inerg interface between the two liquids, allowing rapid transfer of the drugs into the extraction solvent.
Download Chem Data Sheet 140 (PDF) |
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EVOLUTE® CX Columns for Solid Phase Extraction of Basic Compounds from Aqueous Samples
This Chemistry Data Sheet provides guidelines for the extraction of basic analytes from aqueous samples using resin-based mixed-mode SPE.
Download Chem Data Sheet 141 (PDF) |
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ISOLUTE® EPH Solid Phase Extraction Columns for the Fractionation of Extractable Petroleum Hydrocarbons from Soil using both Automated and Manual Procedures
This Chemistry Data Sheet provides for both the automated and manual fractionation of EPH in soil.
Download Chem Data Sheet 142 (PDF)
EVOLUTE® WCX Columns for Solid Phase Extraction of
Quaternary Amines and Strongly Basic Compounds from
Aqueous Samples
This Chemistry Data Sheet provides guidelines for the extraction of quaternary amines and strongly basic analytes from aqueous samples using EVOLUTE WCX
Download Chem Dta Sheet 143 (PDF)
Step by step guide to method development
Download Guide (PDF - 57 K) |
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