IST EVOLUTE ABN: Ultra-clean extracts, generic methodology and high reproducible recoveries from a water-wettable polymer-based sorbent. Biotage IST Sample Preparation Products

EVOLUTE^® ABN - New Column Configurations!
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Ultra-clean extracts, generic methodology
and high reproducible recoveries

EVOLUTE® ABN is an advanced water-wettable polymer-based sorbent that extracts acidic, basic and neutral analytes from biological fluids and other aqueous matrices. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE products provide a highly effective solution to the problems of ion suppression and matrix effects from dirty extracts. These high performance products allow scientists to use a generic approach for a wide range of compounds, thus reducing method development time while producing super clean extracts.

• Minimal ion suppression
• Four times higher signal intensity in LC-MS/MS
• Fast, simple generic methodology
• High, reproducible recoveries for a wide range of analytes

Evolute Graphic - Clean

Figure 1. The optimized pore structure prevents retention of endogenous large molecular weight interferences, allowing analytes to be eluted in extremely clean extracts.

EVOLUTE ABN Sample Preparation Products are available in a range of Standard Configurations.

Ultra-Clean Extracts
EVOLUTE ABN’s optimized pore structure minimizes the retention of high molecular weight endogenous materials present in complex sample matrices. See Figure 1.

The result is cleaner extracts, providing four times higher signal to noise and improved LC-MS/MS quantitation. See Figure 2 below.

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Evolute ABN TN Graphics EVOLUTE_ABN_TN_Fig2.jpg

Figure 2. LC-MS/MS signal (m/z 195>138) for caffeine spiked plasma, compared to spiked mobile phase (flow injection analysis). EVOLUTE ABN gives four times higher s/n than a resin-based product.

Simple and fast method development
EVOLUTE ABN uses a single generic procedure suitable for a wide range of analytes and aqueous matrices. Without secondary interactions, pure organic elution solvents can be used, reducing evaporation time and providing optimal LC-MS/MS compatibility, making the transfer to analysis more efficient.

High analyte recoveries
EVOLUTE ABN has a unique modified surface structure that is water-wettable to promote robust, reliable sample preparation. A balanced combination of polar (hydrophilic) and non-polar (hydrophobic) interactions allows efficient extraction of analytes of wide ranging polarity.

Excellent flow characteristics
EVOLUTE ABN’s narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channeling, which can lead to analyte breakthrough.

Batch-to-batch reproducibility
The surface characteristics of every batch of EVOLUTE ABN are quality controlled and tested to ensure consistency.

Ordering Information

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EVOLUTE ABN 1 mL SPE Columns

Part Number	Description	Quantity
600-0001-A	EVOLUTE ABN 10 mg/1 mL columns	100
600-0002-A	EVOLUTE ABN 25 mg/1 mL columns	100

EVOLUTE ABN Fixed Well Plate

Part Number	Description	Quantity
600-0010-P01	EVOLUTE ABN 10 mg Fixed Well Plate	1
600-0025-P01	EVOLUTE ABN 25 mg Fixed Well Plate	1

EVOLUTE Array ABN Loose Wells

Part Number	Description	Quantity
600-0010-R	EVOLUTE Array ABN 10 mg/1 mL wells	100
600-0025-R	EVOLUTE Array ABN 25 mg/1 mL wells	100

Pre-assembled EVOLUTE Array plates are available.
To order, add the suffix P to equivalent loose well part number.

EVOLUTE Array Accessories

Part Number	Description	Quantity
120-6000-P01	EVOLUTE Array base plate	1
120-1200	Strip of 8 base plate sealing plugs*	50
120-1201	Luer adaptors (to fit any standard sample processing manifold)	25
120-1202	Well removing tool*	1

*Required when processing a partially populated EVOLUTE Array ABN plate.

NEW! EVOLUTE ABN 50 µm Columns and Large Volume 30 µm Formats

Part Number	Description	Quantity
600-0002-G	EVOLUTE ABN 30 µm 25 mg/10 mL (G)	50
610-0005-B	EVOLUTE ABN 50 µm 50 mg/3 mL	50
610-0010-B	EVOLUTE ABN 50 µm 100 mg/3 mL	50
610-0010-H	EVOLUTE ABN 50 µm 100 mg/10 mL (H)	50
610-0020-B	EVOLUTE ABN 50 µm 200 mg/3 mL	50
610-0020-C	EVOLUTE ABN 50 µm 200 mg/6 mL	30

Click here to download Material Safety Data Sheet.