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Benefits
- Minimal ion suppression
- Four times higher signal intensity in LC-MS/MS
- Fast, simple generic methodology
- High, reproducible recoveries for a wide range of analyte
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Ultra-clean extracts, generic methodology
and high reproducible recoveries
EVOLUTE ABN is an advanced water-wettable polymer-based sorbent that extracts acidic, basic and neutral analytes from biological fluids and other aqueous matrices. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE products provide a highly effective solution to the problems of ion suppression and matrix effects from dirty extracts. These high performance products allow scientists to use a generic approach for a wide range of compounds, thus reducing method development time while producing super clean extracts.
EVOLUTE ABN Sample Preparation Products are available in a range of Standard Configurations.
Ultra-Clean Extracts
EVOLUTE ABN’s optimized pore structure minimizes the retention of high molecular weight endogenous materials present in complex sample matrices. See Figure 1.
Figure 1. The optimized pore structure prevents retention of endogenous large molecular weight interferences, allowing analytes to be eluted in extremely clean extra
The result is cleaner extracts, providing four times higher signal to noise and improved LC-MS/MS quantitation. See Figure 2 below.
Figure 2. LC-MS/MS signal (m/z 195>138) for caffeine spiked plasma, compared to spiked mobile phase (flow injection analysis). EVOLUTE ABN gives four times higher s/n than a resin-based product
Simple and fast method development
EVOLUTE ABN uses a single generic procedure suitable for a wide range of analytes and aqueous matrices. Without secondary interactions, pure organic elution solvents can be used, reducing evaporation time and providing optimal LC-MS/MS compatibility, making the transfer to analysis more efficient.
High analyte recoveries
EVOLUTE ABN has a unique modified surface structure that is water-wettable to promote robust, reliable sample preparation. A balanced combination of polar (hydrophilic) and non-polar (hydrophobic) interactions allows efficient extraction of analytes of wide ranging polarity.
Excellent flow characteristics
EVOLUTE ABN’s narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channeling, which can lead to analyte breakthrough.
Batch-to-batch reproducibility
The surface characteristics of every batch of EVOLUTE ABN are quality controlled and tested to ensure consistency.
Ordering Information
| Part Number |
Description |
Qty |
| EVOLUTE ABN 1 mL SPE Columns |
| 600-0001-A |
EVOLUTE ABN 10 mg/1 mL columns |
100 |
| 600-0002-A |
EVOLUTE ABN 25 mg/1 mL columns |
100 |
| EVOLUTE ABN Fixed Well Plate |
| 600-0010-PX01 |
EVOLUTE EXPRESS ABN 10 mg Fixed Well Plate |
1 |
| 600-0030-PX01 |
EVOLUTE EXPRESS ABN 30 mg Fixed Well Plate |
1 |
| EVOLUTE Array ABN Loose Wells |
| 600-0010-R |
EVOLUTE Array ABN 10 mg/1 mL wells |
100 |
| 600-0025-R |
EVOLUTE Array ABN 25 mg/1 mL wells |
1 |
Pre-assembled EVOLUTE Array plates are available.
To order, add the suffix P to equivalent loose well part number. |
| EVOLUTE Array Accessories |
| 120-6000-P01 |
EVOLUTE Array base plate |
1 |
| 120-1200 |
Strip of 8 base plate sealing plugs* |
50 |
| 120-1201 |
Luer adaptors (to fit any standard sample processing manifold) |
25 |
| 120-1202 |
Well removing tool* |
1 |
| *Required when processing a partially populated EVOLUTE Array ABN plate. |
| EVOLUTE ABN 50 µm Columns and Large Volume 30 µm Formats |
| 600-0002-G |
EVOLUTE ABN 30 µm 25 mg/10 mL (G) |
50 |
610-0005-B
|
EVOLUTE ABN 50 µm 50 mg/3 mL |
50 |
| 610-0010-B |
EVOLUTE ABN 50 µm 100 mg/3 mL |
50 |
| 610-0010-H |
EVOLUTE ABN 50 µm 100 mg/10 mL (H) |
50 |
| 610-0020-B |
EVOLUTE ABN 50 µm 200 mg/3 mL |
50 |
| 610-0020-C |
EVOLUTE ABN 50 µm 200 mg/6 mL |
30 |