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Benefits
- Removal of proteins, salts, non-ionizable interferences
and phospholipids
- Minimal ion suppression
- 3 times higher signal intensity
in LC-MS/MS
- Fast, simple generic methodology
- High, reproducible recoveries for a wide range of basic drugs
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Mixed-mode selectivity, generic methodology
and efficient extraction
EVOLUTE CX mixed-mode resin-based SPE sorbent extracts a wide range of basic analytes from biological fluids and other aqueous matrices using a generic procedure which minimizes method development time. EVOLUTE CX removes matrix components such as proteins, salts, non-ionizable interferences and phospholipids, delivering cleaner extracts with reproducible recoveries for accurate quantitation.
EVOLUTE CX sample preparation products are available in a range of standard configurations.
High selectivity provides ultra-clean extracts
The mixed-mode sorbent chemistry and optimized pore size minimizes retention of high molecular weight matrix components present in the biological fluid samples (Figure 1). The interferences including proteins, salts, non-ionizable molecules and phospholipids are removed during the interference elution step, thereby ensuring extremely clean extracts for LC-MS/MS analysis (Figures 2a and 2b).
Figure 1 The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.
Figure 2a Total ion suppression measured by FIA analysis. LC-MS/MS signal (m/z 195>138) for spiked plasma, compared to spiked mobile phase. EVOLUTE gives 3 times higher s/n than other resin-based products.
Figure 2b Chromatographic ion suppression of five basic drugs. LC-MS/MS signal (342>116) for spiked plasma compared to spiked mobile phase.
Simple, fast method development
EVOLUTE CX uses a single generic methodology suitable for a wide range of basic drugs.
High analyte recoveries
EVOLUTE CX has a water-wettable surface to promote robust, reliable sample preparation. The combination of robust polymer chemistry, uniform coverage of sulfonic acid (SO3-) groups and optimum exchange capacity provide greater than 85% recovery for a wide range of basic drugs.
Excellent flow characteristics
Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough.
Batch-to-batch reproducibility
The surface characteristics of each batch of EVOLUTE CX are quality controlled and tested to ensure consistency.
Bioanalysis - EVOLUTE CX 30 µm
Biotage offers a range of EVOLUTE CX columns and 96-well plates optimised for bioanalytical sample preparation.
Forensic, clinical and environmentlal applications - EVOLUTE CX 50µm
EVOLUTE CX is also available in larger SPE formats suitable for the larger sample volumes and more viscous matrices common in forensic, clinical, food and environmental applications.
Ordering Information
| Part Number |
Description |
Qty |
| EVOLUTE CX Fixed Well Plate |
| 601-0010-PX01 |
EVOLUTE EXPRESS CX 10 mg Fixed Well Plate |
1 |
| 601-0030-PX01 |
EVOLUTE EXPRESS CX 30 mg Fixed Well Plate |
1 |
| EVOLUTE Array CX Loose Wells |
| 601-0010-R |
EVOLUTE Array CX 10 mg/1 mL wells |
100 |
| 601-0025-R |
EVOLUTE Array CX 25 mg/1 mL wells |
100 |
Pre-assembled EVOLUTE Array plates are available.
To order, add the suffix P to equivalent loose well part number. |
| EVOLUTE Array Accessories |
| 120-6000-P01 |
EVOLUTE Array base plate |
1 |
| 120-1200 |
Strip of 8 base plate sealing plugs* |
50 |
| 120-1201 |
Luer adaptors (fits standard sample processing manifold) |
25 |
| 120-1202 |
Well removing tool* |
1 |
| *Required when processing a partially populated EVOLUTE Array CX plate. |
| EVOLUTE CX 1 mL SPE Columns |
| 601-0001-A |
EVOLUTE CX 10 mg/1 mL columns |
100 |
| 601-0002-A |
EVOLUTE CX 25 mg/1 mL columns |
100 |
| EVOLUTE CX 1 mL SPE (Tab-less) Columns |
| 601-0001-AG |
EVOLUTE CX 10 mg/1 mL Tab-less SPE columns |
100 |
| 601-0002-AG |
EVOLUTE CX 25 mg/1 mL Tab-less SPE columns |
100 |
| EVOLUTE CX 50 µm Columns |
| 611-0005-B |
EVOLUTE CX 50 µm 50 mg/3 mL SPE columns
|
50 |
| 611-0010-B |
EVOLUTE CX 50 µm 100 mg/3 mL SPE columns |
50 |
| 611-0010-H |
EVOLUTE CX 50 µm 100 mg/10 mL
SPE columns(H)1 |
50 |
| 611-0020-B |
EVOLUTE CX 50 µm 200 mg/3 mL SPE columns |
50 |
| 611-0020-C |
EVOLUTE CX 50 µm 200 mg/6 mL SPE columns |
30 |
| 611-0050-C |
EVOLUTE CX 50 µm 500 mg/6 mL SPE columns |
30 |
| 1 100 mg/10 mL (H) columns have the same sorbent bed dimensions as a 3 mL column, but with an extended reservoir for loading sample volumes up to 10 mL in one aliquot. |
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