Isolera™ Flash Purification System Intelligent features enable better separations in less time with fewer resources.  High performance flash chromatography at its best.
Unique Features: • Quatro-binary Gradient- can use 4 solvents in a single binary gradient • Patented TLC to gradient optimizes sample load		New: Detector Options • ELSD • UV-VIS

Isolera™, a new more compact flash purification system with intelligent features, enables chemists to easily achieve better separations. The advanced TLC-to-gradient feature automatically creates elution gradients and suggests cartridge and sample size. Collect fractions using two wavelengths, adjust the flow rate from 1 to 200 mL/min as needed and use up to four solvents in a single gradient, for maximum purity and yield.

Isolera is available in a single cartridge base model, the Isolera One, and a 4-cartridge configuration, the Isolera Four, which is ideal for multi-user or high-throughput laboratories.

Faster Flow Rates – increase productivity and save valuable time

With a flow range of 1 to 200 mL/min, Isolera dramatically shortens purification run-times. For example, a 10.5 g sample was purified at 200 mL/min on a 340 g Biotage SNAP cartridge in less than 19 minutes, see figure 1. If this same separation had been performed using a typical flash system with a 100 mL/min flow rate limitation, the purification would have taken twice as long.

Figure 1. The value of fast flow rates can be seen in the 10.5 gram purification at 200 mL/min using a SNAP 340g KP-SIL cartridge on an Isolera One. The total run time is 18.8 minutes.


Collect on 2 separate wavelengths – Recover more compounds

Collect eluting compounds that don’t absorb at the primary wavelength using the variable wavelength or UV-VIS diode array detector options. Instead of fractionating on a single wavelength while monitoring a second, Isolera can fractionate simultaneous on two unique user-defined wavelengths. This capability enables chemists to recover more of the compounds and get cleaner fractions, as highlighted in figure 2.



Figure 2. Fractionation using two wavelengths ensures collection of UV absorbing compounds at both wavelengths without sacrificing valuable fraction collection capacity in a “collect all” mode.

Additional features:

• Largest integrated fraction capacity
• Quatro-binary Gradient – Use up to four solvents in a single gradient
• Isocratic co-solvent – increase compound solubility and recovery
• Default Methods – simplify method creation
• On-the-fly editing – modify purification runs in progress
• SNAP cartridges – increase loading capacity and improve compound separations.
• Add an auxiliary detector – Use other detectors
• An assortment of SNAP cartridges is included with the Isolera system
• Connect an external auxiliary detector (refractive index or ELSD)

Specifications