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ExploraSep screening plates support rapid method development and allow identification of selective molecular imprinted polymers MIPs for your impurity or target molecule. ExploraSep is ideal for researchers who require a MIP solution and work with proprietary compounds, or with highly regulated compounds (nerve agents, toxins). The screening tool is also suitable for researchers who frequently have to develop improved highly selective sample preparation or process methods.
Molecular imprinting creates binding sites in an inert polymer with defined functionalities and geometries. These binding sites recognize a range of chemical substructures. ExploraSep technology exploits this in a library of molecularly imprinted polymers with specific selectivity.
The ExploraSep library is packed into a 96-well plate format. This includes molecularly imprinted polymer (MIP) and non-imprinted polymers (NIPs), complemented with a series of unique polymeric phases. The screening may be performed on automated robotic systems or manually using multi-channel pipettes. All ExploraSep plates and resins are re-usable when clean solvents are used (without surfactants or proteins) and can be regenerated. All MIP resins used in ExploraSep plates are available in pre-packed SPE columns and as bulk resin. This means that once a “hit” resin is found by screening, optimization and further method development can be easily completed.
Choosing a Screening Plate
Plate A (Acidic)
Polymers have hydrophobic and carboxylic acid groups as functional moieties and can be used for screening of polar, basic (e.g. amines), neutral (e.g. amides, or esters), and acidic (carboxylic acid) compounds.
Plate U (Urea)
Polymers have hydrophobic moieties and urea groups as functional moieties and are used for screening phosphates, phosphonates, sulphates and sulphonates, peptides, proteins as well as anions of carboxylic acids and lactones.
Plate C (Carbohydrate)
Polymers have both hydrophobic moieties and boronic acid groups that can interact with 1,2- and 1,3-diols, α-hydroxy carboxylic acids, carbohydrates and hydrophilic peptides under basic conditions.
Plate H (Hydrophobic)
Polymers have phenyl or pyridine aromatic moieties that predominantly interact via hydrophobic interactions but that can also form charge transfer complexes and π-π interactions.
Overview of the ExploraSep Process
- Conditioning: This helps activate the binding sites
- Screen: Pass target mixture through plate
- Assay: Apply analytical method to visualize hit
- Regenerate: Prepare plate for next re-use
Format and quantities
ExploraSep phases are packed in 96-well plates (40 mg/well). The materials are spherical with an average particle size of 50-65 µm. ExploraSep plates come with a full user guide and recommended starting protocols. After screening, individual phases can be supplied in larger amounts, from grams to multi-Kg quantities
||ExploraSep Screening Plate A
||Basic and neutral polar
||ExploraSep Screening Plate U
||Aacidic and neutral polar
||ExploraSep Screening Plate C
||Ddiols and neutral polar
||ExploraSep Screening Plate H
||Non-polar and aromatic