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SAM
Multiple Semi-Automated Peptide Synthesizer
SAM, your guarantee for optimal peptide synthesis
The Biotage® SAM is a semi-automated multiple (parallel) peptide synthesis system that can produce 24 peptides in parallel.
The 24-position U-block reactor can be operated with 2 mL, 5 mL, or 10 mL disposable polypropylene reactors (with PTFE frits) for FMOC-synthesis between 5-300 micromole scale. Four different solvents and reagents can be pumped into the reaction flask. A digital syringe pump provides accurate dispensing of reagents. A special dispensing plate allows the reagents and solvents to be distributed into the reactor vials. The liquid distribution proceeds in parallel for 6 reactors at a time. The infinitely variable vortexer guarantees a homogeneous mixing of the reactants. The SAM is controlled through an external controller. The controller is equipped with an LCD display with 40 x 16 characters and six function keys, and is easy to use and stores 10-programs each with up 20-steps.
System Components
- Unit with diluter and integrated 8-way valve
- Controller
- Vortexer
- Washing Plate
- U-Type Reactor block
- Vacuum-Pump ME2C
- 10 L waste bottle
Flexibility and User-friendliness
All important system control functions can be used directly in manual mode without a long familiarization period with the complicated control software. Complex single step sequences are linked to a single key command. Complete syntheses-cycles can be processed automatically by linking up to 20 key commands to a program sequence. Ten of these individual programs can be saved on the SAM and remain available to the user.
Controller
The controller for the SAM is equipped with a 40 x 16 character LCD display and six function keys. The function keys can be used for particular commands as well as the creation and execution of complex programs. For the manual addition of amino acids, we have written a simple PC program that creates and prints a pipetting raster which helps make pipetting safer.
Software
- Start up screen
- General function keys
- System settings
- Programming menu
- Menu for manual operation
System Specifications
| Synthesizer |
Plastic box
Digital syringe pump
Syringe size 10 ml
8-way-valve 4 outlet ports and 4 reagents/solvents ports
Vortexer 24 V, 100–600 rpm
Reaction block U-type 24 positions
Dispensing plate
3-way Teflon-valve |
| Reaction Vessels |
PP-reactors with PTFE-frits
Reactor size 2 mlL 5 mL, 10 mL
Synthesis scale between 0.05 and 0.3 mmol |
| Vacuum |
Vacuum membrane pump ME2 C
10L Nalgene vacuum waste bottle |
| Controller |
Microprocessor
LC-display with 40x16 characters
10 program files with 20 steps
Automatic fill and wash procedures
Manual mode |
Power Requirements
|
Input voltages: 110-220 VAC +/- 10 %
Power supply 24 V max. 150 W |
Dimensions
WxDxH |
Synthesizer: 540 mm x 250 mm x 345 mm
Controller: 195 mm x 65 mm x 170 mm |