Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: TN125Issued year: 2003File size: 0.07mbFile type: pdf
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Part No: TN126Issued year: 2003File size: 0.06mbFile type: pdf
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Part No: Issued year: 2007File size: 0.48mbFile type: pdf
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Part No: TN127Issued year: 2003File size: 0.28mbFile type: pdf
ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract.
Part No: Issued year: 2004File size: 0.31mbFile type: pdf
1.We have developeda general method for synthesizing Diaryl Ethers from both electron richand electron deficient phenols.
2.We have showedthat with our instrumentation we have removedscalabilityas alimiting factor for the ”bench” chemist.
3.We have also developeda new cooling technique on the Advancer that dramaticallyshortens coolingtimes. For ethanol: 180 ºC to 70 ºC within less than 10 seconds..
Part No: P020Issued year: 2008File size: 0.16mbFile type: pdf
The trend pharma industry is clearly to speed up the process from lead identification to selection of candidate drug. One of the major limitations is the availability of larger amounts typically hundreds of grams, of the potentially active substance to be further investigated
Part No: P153.v.1Issued year: 2017File size: 1.12mbFile type: pdf
This poster describes a simple but effective method for screening a range of pesticides and drugs of abuse from liver samples. Liver tissue is homogenized using the Biotage® Lysera, and the subsequent extract is cleaned up using ISOLUTE SLE+ supported liquid extraction columns.
Part No: P012Issued year: 2006File size: 0.22mbFile type: pdf
The use of palladium-catalysts in the synthesis of fine chemicals, pharmaceutical intermediates and active pharmaceutical ingredients (APIs) has become quite common in the last few decades. The number of palladiumcatalyzed reactions (both achiral and chiral) available to chemists has provided access to more complex structures in fewer steps and with less waste. An unfortunate side effect of using palladium is the potential for palladiumcontaining impurities to remain in the desired compound after isolation. This is an especially significant problem for the pharmaceutical industry since there is a low limit for heavy metal impurities allowed in the drug substance.
Part No: AN072.v2Issued year: 2013File size: 0.39mbFile type: pdf
Here we present the separation capabilities of a new type of designed adsorbent, RENSA® Boronate. This new resin has affinity for diols and α-hydroxycarboxylic acids and is able to extract those target compounds with high efficiency and selectivity.
Part No: Issued year: 2010File size: 0.26mbFile type: pdf
• Solid-Phase Peptide Synthesis (SPPS) is still often
faced with difficulties in the assembly of long and
‘difficult’ sequences, e.g. due to aggregation and
steric hindrance giving rise to incomplete reactions.
These problems have only partly been solved by
new coupling reagents and solid supports.
• Precise microwave heating has emerged as one
new parameter for SPPS, in addition to coupling
reagents, resins, solvents etc.
Part No: Issued year: 2017File size: 0.31mbFile type: pdf
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
Part No: AN091Issued year: 2014File size: 0.63mbFile type: pdf
Three protected carbohydrate derivatives, 1,2:5,6-Di-O-Isopropylidene-
α-D-glucofuranose, Methyl 4,6-O-Benzylidene-α-D-glucopyranose, and
1,2-O-Isopropylidene-α-D-glucofuranose were successfully separated and
detected, using a Biotage® SNAP KP-Sil cartridge on a Biotage Isolera™ system
with the Biotage® Evaporative Light-Scattering Detector (ELSD) attached.
Part No: AN089Issued year: 2014File size: 0.68mbFile type: pdf
Three sterols, (+)-dihydrocholesterol, β-estradiol-3-benzoate, and
β-estradiol were successfully separated, detected, and isolated using a
Biotage® SNAP KP-Sil cartridge attached to a Biotage Isolera™ One system connected to Biotage® Evaporative Light-Scattering Detector (ELSD).
Part No: Issued year: 2007File size: 3.19mbFile type: pdf
Biotage bietet ein umfassendes Jahresservice-Konzept, inhaltlich aller Ersatzteile, Arbeit vor Ort und empfohlener vorbeugender Wartung. Holen Sie sich den Prioritätsstatus für alle Service-Anrufe durch spezialisierte Außendiensttechniker von Biotage und mit
Original-Biotage-Ersatzteilen (wesentlich zur Aufrechterhaltung der optimalsten Geräteleistung).
Part No: Issued year: 2007File size: 0.05mbFile type: pdf
PS-TsNHNH2 and ISOLUTE® Si-TsNHNH2 are solid supported equivalents of p-toluenesulfonyl hydrazide. These reagents are both excellent scavengers for aldehydes and ketones and are generally used in threefold excess with respect to the limiting carbonyl species. A catalytic amount of acetic acid (5-10%) may be required for ketones, hindered substrates or for scavenging in certain solvents (e.g. DMF).
Part No: P022Issued year: 2008File size: 0.09mbFile type: pdf
Microwave-assisted esterification of protected amino acid derivatives was performed using alkyl halides and polystyrene supported TBD1,2 (Fig. 1) as base. The preferred solvents were THF or acetonitrile. The products were obtained in good yields and high purity. Short reaction time, 10 minutes, and easy work-up, filtration and evaporation, constituted a convenient procedure. Benzyl, allyl, phenacyl, and alkyl esters, all important and commonly used derivatives in peptide synthesis, were synthesised.
Part No: P155Issued year: 2017File size: 0.61mbFile type: pdf
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Part No: P152Issued year: 2016File size: 0.49mbFile type: pdf
This poster describes a method for the simultaneous extraction (using EVOLUTE EXPRESS WCX) and analysis of plasma catecholamines and metanephrines. A highly sensitive, linear and rugged assay was developed, and automated using the Biotage Extrahera sample preparation processing system.
MSACL EU 2016
Part No: AN755Issued year: 2012File size: 1.03mbFile type: pdf
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats.
EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,
Part No: AN098Issued year: 2014File size: 0.81mbFile type: pdf
To demonstrate the cost savings by employing small μmol scale automated solid phase peptide synthesis through robot liquid handling of reagents with accurate dispensing of small volumes, a 10-mer antimicrobial peptide was synthesized.
Part No: Issued year: 2008File size: 0.13mbFile type: pdf
The adapter kit contains a pre-assembled stand, spacer and
connecting tubing; everything required to allow the use of
SNAP 340g cartridges on flash purification systems that utilize
Luer tubing connections such as, Isco Companion and Analogix
Part No: install_snap_25.zipIssued year: 2012File size: 0mbFile type: zip
7742_install_snap_25.zip. SPX 25 g SNAP cartridge installation.
The system already has the SNAP 10 g, 50 g, 100 g and 340 g cartridges installed
(only the SNAP 25 g needs to be installed)
Part No: install_snap_full.zipIssued year: 2012File size: 0mbFile type: zip
install_snap_full.zip. SNAP Software Update for Biotage® SP Systems. SPX FULL SNAP cartridge installation instructions.
The systems that does not have any SNAP cartridges installed
(the SNAP 10 g, 25 g, 50 g, 100 g and 340 g will be installed)
Part No: TN-SNAP XL-0211Issued year: 2011File size: 1.22mbFile type: pdf
The SNAP XL cartridges address a growing need for optimized gram- to ~100+ g-scale purification, increasing efficiency and reducing costs. These cartridges extend the
SNAP product ranges, providing chemists with more scalable tools for their synthesis and purification needs. There are 3 members in this family of cartridge solutions, KP-Sil for normal phase, C18 for reverse phase and KPNH for more difficult normal phase systems, such as amines. SNAP XL cartridges will allow you to save hours, even days of purification run-time.
Part No: TN114Issued year: 2006File size: 0.12mbFile type: pdf
New approaches to the quantitation of drugs and metabolites in biological fluids have increased the need for high throughput methodologies to avoid the traditional laboratory bottleneck of sample preparation. Solid phase extraction in a 96-well plate format such as ISOLUTE-96 Fixed Well Plates or ISOLUTE Array 96-well Plates is a solution to this problem.
Part No: Issued year: 2013File size: 2.08mbFile type: pdf
Amide-linked homopolymers of sialic acid offer the advantages of stable secondary structure and increased bioavailability making them useful constructs for pharmaceutical design and drug delivery. Defining the structural characteristics that give rise to secondary structure in aqueous solution is challenging in homopolymeric material due to spectral overlap in NMR spectra. Having previously developed computational tools for heteroologomers with resolved spectra, we now report that application of these methods in combination with circular dichroism, NH/ND NMR exchange rates and nOe data has enabled the structural determination of a neutral, d-amide-linked homopolymer of a sialic acid analogue called Neu2en.
Keywords: sialic acid; Neu2en; NMR; circular dichroism; helical peptide; water-soluble scaffold; blood serum; proteolysis; 1,3-dipolar cycloaddition chemistry; click chemistry; Initiator+ SP Wave
Jonel P. Saludes, Travis Q. Gregar, I. Abrrey Monreal, Brandan M. Cook, Lieza M. Danan-Leon, Jacquelyn Gervay-Hague
Part No: Issued year: 2007File size: 0.22mbFile type: pdf
Biotage has created a new Resin-based Chemistry Kit that contains a variety of resins
designed to enable a choice of key resin-assisted chemistries with optimized protocols
(Figure 1). Reductive amination, coupling (C-C or C-O bond formation), amide bond
formation and oxidation are widely used traditional chemical transformations you’ll be able
to perform with this new kit.
Part No: TN-0021.0207Issued year: 2007File size: 0.34mbFile type: pdf
Resins are usually described as one of 2 main types, Gel (PS) or macroporous (MP).
The gel resins have a low cross-linking and need to swell to allow access to their
functional groups. Therefore, they are very effective in transformations requiring
swelling organic aprotic solvents such as DCM, THF, Dioxane etc. Conversely, MP
resins are more highly cross-linked with more of a permanent open pore structure,
so they do not require swelling in such solvents for effective performance. As a
result, MP resins are compatible with a wider range of solvents than PS resins and
perform better in solvents such as water, MeOH and MeCN. Resins are generally
stable and can be stored indefinitely under cool dry conditions. For longer term
storage standard refrigeration (4 °C) is recommended.
Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications.
Keywords: turbovap, turbo vap, v10, v-10, spedry, spe dry, evaporation, concentrat
Part No: AN037Issued year: 2001File size: 0.13mbFile type: pdf
Activated carbon is an adsorbent media used to remove colored compounds from solution. Using carbon in a packed bed, such as the Biotage FLASH-AC cartridge improves adsorbent performance. The key variable in optimizing the decolorization process is the solvent system. Varying the solvent system impacts the adsorbent capacity of the cartridge, affecting both the yield and purity of the product. Biotage FLASH-AC cartridges tolerate a wide range of process solvents. This note discusses solvent choice as a process variable and how it contributes to significant process improvement and reduces the total cost of operation.
Part No: IST1080Issued year: 2006File size: 0.1mbFile type: pdf
SPE Application for the Multiresidue Extraction and Clean Up from Fruit and Vegetables. The method can be used for the extraction of a range of commodities (fresh fruits and
vegetables) and is suitablefor analysis of organophosphorus, organochlorine and carbamate pesticide types, using a variety of appropriate analytical techniques.
Part No: IST1057AIssued year: 1999File size: 0.07mbFile type: pdf
The following method addresses the extraction of 6-monoacetyl morphine from urine using
non-polar and strong cation exchange retention mechanisms. This compound can confirm
heroine use since it is a heroine metabolite, but not a metabolite of codeine or morphine
Part No: IST1002AIssued year: 2011File size: 0.04mbFile type: pdf
This method has been optimized for the extraction of cocaine and benzoylecgonine from urine for drugs of abuse applications. Typical recoveries of these drugs are > 85%. The procedure may be applied to plasma, serum or whole blood with suitable modifications.
Part No: IST1006AIssued year: 2008File size: 0.04mbFile type: pdf
This method is suitable for the extraction of a wide range of analytes, as defined in EPA methods 525.2 and 8080, from water using a non-polar retention mechanism. These include PAHs, triazine herbicides, organochlorine pesticides, phthalates and other extractables.
Part No: IST1042AIssued year: 2006File size: 0.07mbFile type: pdf
This method was developed for the extraction of total petroleum hydrocarbons (silica gel treated-hexane extractable material (SGT-HEM) as described in EPA method 1664) from water samples. The analytes are determined by gravimetric analysis.
Part No: IST1018AIssued year: 2005File size: 0.04mbFile type: pdf
This method was developed for extraction of total petroleum hydrocarbons (or silica gel treated-hexane extractable material (SGT-HEM) as described in EPA method 1664) from water samples using the AutoTrace system.
Part No: IST1005AIssued year: 2008File size: 0.03mbFile type: pdf
This method is applicable to the determination of Oil and Grease as defined by EPA Method 1664 as Hexane Extractable Material (HEM, also referred to as Total Oil and Grease) as well as Silica Gel Treated-Hexane Extractable Material (SGT-HEM, also referred to as Total Petroleum Hydrocarbons or TPH).
EPA, Fractionation, EPA Method 1664, TPH, Total Petroleum Hydrocarbons
Part No: AN-IST1007SIssued year: 2008File size: 0.04mbFile type: pdf
This method described the use of a non-polar SPE column for the extraction of pyridoxine, riboflavin and thiamine from biological fluid samples. This represents recommendations for SPE method development. The proposed steps are based on experience with similar analytes and matrices, but have not been verified in Biotage laboratories. Please refer to section below for the analyte and matrix considerations that were made in developing this method. As for all method development, this procedure should first be developed using pure solvent spiked with analyte. Only after the chemistry is established should spiked matrix samples be tested.
Part No: AN-IST1010SIssued year: 2008File size: 0.05mbFile type: pdf
This method describes the exxtraction of methamidophos and carbofuran from water using a non-polar retention mechanism. This represents recommendations for SPE method development. The proposed steps are based on experience with similar analytes and matrices, but have not been verified in Biotage laboratories. Please refer to section below for the analyte and matrix considerations that were made in developing this method. As for all method development, this procedure should first be developed using pure solvent spiked with analyte. Only after the chemistry is established should spiked matrix samples be tested.
Part No: IST1013SIssued year: 2005File size: 0.03mbFile type: pdf
This represents recommendations for SPE method development. The proposed steps are
based on experience with similar analytes and matrices, but have not been verified in
Biotage laboratories. Please refer to section below for the analyte and matrix
considerations that were made in developing this method. As for all method development, this procedure should first be developed using pure solvent spiked with analyte. Only after the chemistry is established should spiked matrix samples be tested.