Part No: AN068Issued year: 2013File size: 0.6mbFile type: pdf
The ability to synthesize peptides in a range of different
scales is a requirement in many research laboratories. The
Biotage® Initiator+ Alstra™ fully automated microwave peptide
synthesizer has a scale range of 5 μmol up to 2 mmol using
three different reactor vial sizes (5 mL, 10 mL and 30 mL).
The acyl carrier protein fragment, ACP (65–74), H-Val-Gln-
Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is
a well-known so-called difficult sequence and is commonly
used to evaluate the performance of new synthesis reagents
Here we demonstrate a variable scale synthesis on the
Initiator+ Alstra using the ACP (65–74) fragment as a model
Part No: PPS397Issued year: 2016File size: 0.23mbFile type: pdf
Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: AN064Issued year: 2012File size: 1.09mbFile type: pdf
This application note describes the use of ISOLUTE® HM-N as a support material
for pre-adsorption of a reaction mixture prior to flash chromatography. This
approach is useful when loading reaction mixtures that are only soluble in polar
solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.
Part No: AN108Issued year: 2015File size: 0.38mbFile type: pdf
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Part No: AN109Issued year: 2015File size: 0.71mbFile type: pdf
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Part No: AN077Issued year: 2013File size: 1.35mbFile type: pdf
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Part No: P098Issued year: 2014File size: 0.28mbFile type: pdf
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.
Part No: AN085Issued year: 2014File size: 0.92mbFile type: pdf
Mass-directed flash purification is a simple and effective
technique to detect and fractionate compounds with poor UV chromophores. In this study, fatty acid methyl ester mixtures were purified by mass-directed flash purification using the Biotage Isolera™ Dalton System.
Part No: P099Issued year: 2014File size: 0.39mbFile type: pdf
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Part No: PN38Issued year: 2011File size: 0.17mbFile type: pdf
Mephedrone (MCAT) is a ‘designer drug’ synthesised to mimic the amphetamine class of compounds. Due to its perceived ‘ecstasy like’ effects of euphoria, excitement and alertness combined with ease of availability, this (until recently) unregulated drug (legal high) has found widespread abuse in the UK in recent years. Harmful effects associated with mephedrone are still being studied but after several deaths were linked to the abuse of the drug, the UK Government made this a Class B substance. Mephedrone extraction was evaluated on three mixed mode cation exchange SPE sorbents: EVOLUTE® CX, EVOLUTE® WCX and ISOLUTE® HCX.
DOA, ISOLUTE, EVOLUTE, Amphetamine
Part No: Issued year: 2011File size: 0.44mbFile type: pdf
User Report: Metal scavenger Si-Thiol. Nippon Shinyaku Co., Ltd use Biotage metal scavengers to control residual metals such as palladium, which are difficult to remove by other methods. We have been talking to Mr. Toshio Fujiwara who is in charge of Process Chemistry, CMC Research & Development Department, Discovery Research Laboratories.
Part No: P148Issued year: 2016File size: 0.93mbFile type: pdf
Biotage metal scavengers are a more effective alternative to traditional carbon batch adsorption processes. They have the lowest metals content, and also excellent extractable profiles, therefore not likely to risk contamination, unlike competitive materials we tested. Furthermore, Biotage metal scavengers offer volumetrically, a much more compact solution than carbon, requiring 48 times less volume for the same degree of Pd removal in our studies.
Part No: AN102Issued year: 2015File size: 1.12mbFile type: pdf
This study was conducted in 3 parts.
Metal scavengers were investigated for inherent metals content by ICP analysis methods.
Additionally these scavengers were extracted in a range of common solvents in order to show extractable/leachable levels.
We demonstrate an example metal scavenging mitigation step, looking at the corresponding API mass yield, purity and also metals data.
Part No: Issued year: 2013File size: 2.62mbFile type: pdf
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
» Isocratic separations are easiest to develop, but gradient separations are
» Software in the Isolera helps with conversion of an isocratic separation to
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
Part No: TN119Issued year: 2006File size: 0.11mbFile type: pdf
This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3).
Part No: TN108 rev1.3Issued year: 2004File size: 0.13mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE CBA for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchange sorbents (pages 4-6).
Part No: TN104Issued year: 2004File size: 0.06mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE NH2 for the extraction of anionic analytes from aqueous samples (pages 1-2), order information (page 3) and a general discussion on anion exchangers (pages 4-5).
Part No: TN105 rev1.3Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes), specifics on the use of ISOLUTE® PSA for the extraction of anionic
analytes from aqueous samples (page 1-2), ordering information (page 3) and a general discussion
on anion exchangers (pages 4-5). For information on extraction of anionic analytes from nonaqueous
matrices, contact Argonaut Technologies.
Part No: TN103Issued year: 2004File size: 0.07mbFile type: pdf
This technical notes includes specifics on the use of ISOLUTE SAX and PE-AX for the extraction of anionic analytes from acqueous samples (pages 1-3), ordering information (page 4) and a general discussion on anion exchangers (pages 5-6).
Part No: TN106 rev1.3Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE SCX and SCX-3 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchangers (pages 4-5).
Part No: TN107 rev1.4Issued year: 2004File size: 0.06mbFile type: pdf
This technical note includes, specifics on the use of ISOLUTE SCX-2 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchanger sorbents (pages 4-5).
Part No: TN102Issued year: 2006File size: 0.11mbFile type: pdf
This Chemistry Data Sheet includes specific information on the use of polar ISOLUTE® sorbents for the extraction of polar analytes from non-aqueous samples (pages 1-2), and a general discussion on polar sorbents
Part No: P119Issued year: 2015File size: 0.45mbFile type: pdf
This poster describes the supported liquid extraction method development process for the extraction of the NBOMes group of novel psychoactive substances from oral fluid, for analysis using GC-MS. both sample pre-treatment and elution solvent were optimised, and the final method compared to traditional LLE and SPE procedures.
Part No: IST1022Issued year: 2006File size: 0.03mbFile type: pdf
Method Development Recommendations for Aflotoxins from Cereals. This represents recommendations for SPE meth
od development. The proposed steps are
based on experience with similar analytes an
d matrices, but have not been verified in
Part No: P065Issued year: 2014File size: 3.65mbFile type: pdf
This poster demonstrates method development strategies for drugs of abuse screening using supported liquid extraction in a 96-well plate and subsequent method scalability to column format to allow larger matrix volume processing prior to UPLC-MS/MS analysis.
Part No: AN726Issued year: 2011File size: 0.17mbFile type: pdf
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Part No: AN727Issued year: 2011File size: 0.11mbFile type: pdf
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Part No: AN728Issued year: 2011File size: 0.15mbFile type: pdf
Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid.
Cyclosporine, Cyclosporine, Immunosuppressant,
Part No: AN729Issued year: 2011File size: 0.18mbFile type: pdf
This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation.
Opiates, drug testing, DOA, Ion exchange
Part No: AN725Issued year: 2011File size: 0.16mbFile type: pdf
This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.
Cocaine, BZE, Benzoylecgonine, drug testing, cocaine metabolite,
Part No: P092Issued year: 2014File size: 0.36mbFile type: pdf
The fermentation process associated with the manufacture of food grade enzyme powders was recently determined to support the colonization of bacteria. To control this issue, manufactures following different international regulatory procedures have found the addition of familiar antibiotic compounds to manufacturing processes beneficial. This becomes an issue of public health as the concentration of antibiotic residues in food grade materials promotes unknown exposure to the global community. Continued interest in the biomonitoring of these compounds have inspired a number of method development strategies; however, classic methods are labor intensive and require multi-step time consuming efforts. The FDA has recently mandated a method for the determination of chloramphenicol in shellfish. This study evaluates the feasibility of method modifications to incorporate enzyme powder matrices into this method. Linearity was determined over a range of 0.1 – 5 ng/mL (r^2 > 0.990). Relative recovery was determined >80%. The typical repeatability (%RSD) for n=7 replicates <20%. Strategies to control solids prior to SPE load will be presented as they were determined critical in method performance. Method robustness was determined by evaluating multiple lots and multiple variants of enzyme powder origin. The key variable is the nature of the excipients as high level sugars of different sources need to be controlled to minimize the ESI suppression.
Part No: Issued year: 2016File size: 2.34mbFile type: pdf
This poster describes a validated LC-MS/MS method for the quantification of nicotine, its metabolites: cotinine, trans-3’-hydroxycotinine, and the tobacco plant alkaloid anabasine in patient urine to determine their clinical utility for the evaluation of active smokers for pre-transplant eligibility.
Disclaimer: Publication of this poster in no way implies or construes endorsement of Biotage products by Emory University School of Medicine.
Part No: P146Issued year: 2016File size: 0.6mbFile type: pdf
This poster compares various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of extraction techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression, phospholipid removal and simplicity of transfer to an automated sample preparation platform.
Part No: P006Issued year: File size: 0.09mbFile type: pdf
The formation of 2,5-diketopiperazines from a water suspension of N-Boc protected dipeptide alkyl esters or dipeptide alkyl esters was induced by microwave assisted heating (Scheme 1). The products, which precipitated, were obtained in good yields. However, epimerisation/diastereomer formation was found to various extents depending on the amino acid sequence and the applied reaction temperature.