Part No: AN058Issued year: 2012File size: 0.21mbFile type: pdf
The formation of diarylamines by reacting anilines with aromatic electrophiles is, usually, a reaction demanding long reaction
times, high temperatures and strong bases, in order to obtain acceptable yield, due to the low nucleophilicity of anilines.1 The Buchwald-Hartwig cross-coupling reaction is a very useable and elegant solution to this problem.2,3,4 Anticipating the classical metal-free SNAr-amination reaction, without using Pd-catalysts and strong bases, with only higher temperature and pressure as tool as depicted in the Scheme below,1 gives no product at all at 180 ºC and very low conversion (9 %) at 220 ºC for 15 minutes when heating by microwaves.5 Remainder was unreacted starting material.
Part No: 0021304_02Issued year: 2016File size: 0.27mbFile type: pdf
Horizon Technology products revolve around making oil and grease analyses and semivolatile organic extractions safer, faster, more accurate and reproducible. Best of all, with reduced solvent usage, costs are reduced! Coupled with rugged hardware, applications and support, your lab can be more productive than ever before.
Part No: AN903Issued year: 2007File size: 0.1mbFile type: pdf
A one-pot process for the preparation of Acyclovir from guanine is described. The key step involves a trimethylsilyl triflate (TfOTMS)-catalysed reaction of silylated guanine with 1,3-dioxalane under microwave irradiation. With this method, the yield is in the same range as with conventional heating, but the speed of the reaction is increased by a factor of 85.
Part No: P161Issued year: 2017File size: 0.76mbFile type: pdf
Hair is a useful matrix for drug analysis, as it can be used to show historical drug use. Parent drugs are often present in high concentrations, and the matrix is stable over time.
This poster describes the use of the Biotage® Lysera to pulverise and extract hair samples, with ISOLUTE® SLE+ supported liquid extraction columns to clean up the samples prior to analysis. Limits of quantitation set by the Society of Hair Testing for amphetamines are achievable with the described method (0.2 ng/mg).
SoHT 2017, Cardiff UK
Part No: PPS428Issued year: 2016File size: 0.85mbFile type: pdf
This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.
Part No: P141Issued year: 2016File size: 0.33mbFile type: pdf
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
Part No: P130Issued year: 2015File size: 0.48mbFile type: pdf
The results from this research show that the chromatographic purification of organic amine compounds can be improved simply by using an amine-functionalized silica and typical normal-phase solvents such as hexane and ethyl acetate.
Part No: PPS418Issued year: 2016File size: 5.52mbFile type: pdf
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Part No: CM-PAL-0810Issued year: 2010File size: 0.29mbFile type: pdf
PAL-ChemMatrix is highly recommended for microwave synthesis.
Under microwave heating conditions, such as Biotage Initiator
the PAL-ChemMatrix provides higher purity peptides than the
Rink Amide ChemMatrix. For manual synthesis or non-microwave
synthesis, we recommend using the Rink Amide ChemMatrix
Part No: AN099Issued year: 2015File size: 0.36mbFile type: pdf
A series of eight 3-alkylidylindolin-2-ones were conveniently synthesized on Biotage® Endeavor™ in high yield and purity. Reactor purging, agitation, pressurization, heating, gas uptake and run termination were performed automatically based on the run program.
Biotage has developed a range
of tools to improve the peptide synthesis workflow,
from synthesis of crude peptides through to the final
purified product. These solutions make your workflow
more efficient and improve the quality of peptides
you synthesize. Our workflow solutions are used in
pharma, biotech, CRO and academic laboratories
throughout the world.
Part No: PPS439Issued year: 2017File size: 0.7mbFile type: pdf
Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where
they synthesize complex molecules for chemical biology applications. An important tool that
contributes to their success has been Biotage® Initiator+ Alstra™ microwave peptide synthesizer,
and they are even breaking the dogma that only HPLC can be used for peptide purification, by
using cutting edge reversed phase flash purification techniques provided by Biotage.
Part No: I-Prime_ZIP_TN-30.1011Issued year: 2011File size: 0.3mbFile type: pdf
Biotage ZIP flash chromatography cartridges are engineered to meet the needs of chemists requiring high performance and consistency from a purification cartridge at a value price to reduce purification costs.
Isolera Prime flash purification system.
Part No: P023Issued year: 2008File size: 0.37mbFile type: pdf
Sample preparation is an essential technique prior to LC-MS/MS analysis of drugs in biological fluid samples to remove unwanted matrix components. These matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely
clean extracts. This poster investigates the performance of EVOLUTE™ CX for overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.
Part No: Issued year: 2005File size: 0.44mbFile type: pdf
PS-HOBt(HL) is a higher loading version (0.9 – 1.4 mmol/g) of a sulfonamide-linked,
resin-bound equivalent of 1-hydroxybenzotriazole (HOBt). PS-HOBt(HL) can be used as an effective activating reagent for transfer of protecting groups, e.g., Fmoc and Cbz, to amines.
Part No: Issued year: 2007File size: 0.26mbFile type: pdf
PS-TBD is a polymer-supported base, which
consists of a bicyclic guanidine moiety (1,5,7-
triazabicyclo[4.4.0]dec-5-ene) anchored on
polystyrene. PS-TBD is a stronger polymersupported
base than the PS-DIEA and PS-NMM
resins, which are bound tertiary amines that are
used in organic synthesis and reagent
scavenging. Published applications of PS-TBD
include alkylation of phenolsi and amines;ii
esterification of carboxylic acids using alkyl
halides; alkylation of activated methylene
compounds; de-halogenation of organic
halides;iii high throughput synthesis of aryl
triflates and aryl nonaflates,iv and the
regioselective synthesis of lysophospholipids.
Part No: Issued year: 2005File size: 1.52mbFile type: pdf
PS-DEAM is a polystyrene-bound diethanolamine, which is a resin-bound equivalent of the tridentate (N, O, O) N-alkyldiethanolamine ligand. PS-DEAM is an effective scavenger for a variety of oxophilic organometallic and inorganic complexes. The resin can be used to quench reactions and remove metallic reagents, catalysts or byproducts, allowing the purified product to be isolated by filtration. Boronic acids are scavenged by PS-DEAM. This has been exploited in the purification of Suzuki reactions through the removal of excess boronic acid and coupling byproducts.
Part No: Issued year: 2005File size: 0.38mbFile type: pdf
MP-Borohydride is a macroporous, polystyrene-bound borohydride, that is a resin-bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones. The resin, in conjunction with some transition metal salts, can also be used for a number of other important reductive applications, such as reduction of oximes,
azides and alkyl halides. The reduced products are isolated by simple filtration away from the resin.
Part No: P179Issued year: 2018File size: 2.93mbFile type: pdf
This poster discusses the effect of various sample preparation protocols of the recovery of a range of different Drugs of abuse classes, extracted from oral fluid collected using the Quantisal collection device.
MSACL NA 2018, Palm Springs CA
ASMS 2018, San Diego, CA
Part No: PPS476Issued year: 2018File size: 3.07mbFile type: pdf
Oral fluid is of increasing importance as a biological
matrix in drugs of abuse and related analyte testing
because of the ease of sample collection, and
difficulty in adulteration of samples, particularly
when compared with urine.
Part No: AN800Issued year: 2003File size: 0.05mbFile type: pdf
The automated Advantage Series™ 3400 ChemCorder™ workflow replicator was used to record all the manual operations a chemist performs during a highly exothermic chemical reaction (Figure 1).Before the experiment was initiated, the ChemCorder replicator was put in record mode.All steps of the experiment were accurately recorded, including any comments and observations. Once completed the ChemCorder replicator produced a report and recipe file of the chemistry. This recipe file was used to accurately and automatically repeat the reaction. Alternatively, the file could have been modified to produce a new recipe based on the recorded chemistry
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates,
1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.
Part No: Issued year: 2005File size: 0.43mbFile type: pdf
PS-DMAP is a polymer-bound equivalent of dimethylaminopyridine (DMAP) which may be used as a catalyst for acylation and related reactions. PS-DMAP has a loading of 1.4 mmol/g based on nitrogen analysis. Typical catalysis conditions require 10 - 20 mol% relative to the nucleophile. Catalytic PS-DMAP accelerates the acylation of sluggish nucleophiles, e.g. tertiary alcohols.
Part No: Issued year: 2007File size: 0.09mbFile type: pdf
PS-TBD (Figure 1) is a polystyrene bound guanidine base, which is stronger than corresponding resin bound DIEA (DIPEA) and bound N-Methyl-morpholine resins. It has a wide variety of uses in synthesis, from direct alkylation through to sequestering of acidic components of reaction.
Part No: P005Issued year: File size: 1.34mbFile type: pdf
An oxidation method using a novel solid phase oxidant, based on a covalently polystyrene bound, sulfonic ester linked 2,2,6,6-tetramethylpiperidine-1-oxyl species is presented, PS-TEMPO (Fig.1a&1b).1,2 The reagent showed fast room temperature kinetics compared to similar known systems3 when used to oxidize primary and secondary alcohols to aldehydes and ketones, respectively (Table 1). The products were in general of high purity and the isolated yields were good to excellent. The used reagent could be reactivated and reused for multiple oxidation reactions. The colour change observed as the oxidation state of the reagent changed, gave an indication of the extent to which the reagent had been used.
Part No: TN521Issued year: 2003File size: 0.05mbFile type: pdf
PS-Thiophenol is based on an aminomethyl resin with a tethered thiophenol functionality. The resin was
designed for the scavenging of alkylating agents, e.g. alkyl halides. PS-Thiophenol was tested and found
effective in scavenging alkylating agents ranging from octyl bromide to benzyl bromide. The scavenging
effectiveness of PS-Thiophenol was found to be greater than a polymer-bound benzyl thiol towards octyl
bromide, indicative of the higher nucleophilicity of the thiophenolate.
Part No: TN513Issued year: 2003File size: 0.03mbFile type: pdf
PS-Trisamine is an amine functional resin for the removal of excess electrophilic reagents during the quenching and purification of reaction mixtures.1 PS-Trisamine resin has a scavenging capacity of 3.0–4.0 mmol/g based on reaction with an excess of benzoyl chloride. Scavenging of common electrophiles typically requires 3–6 equiv of PS-Trisamine and occurs within 0.5–3 h at room temperature. The scope of PS-Trisamine as a scavenger for electrophilic reagents was investigated using 4-chlorobenzoyl chloride, 2-phenylbutyryl chloride and 2,6-dimethoxybenzoyl chloride as a series of acid chlorides with decreasing
reactivity.Acid chlorides were completely scavenged in 0.5 h using 3.5 equiv PS-Trisamine (Table 1). The presence of a tertiary base, e.g. PS-DIEA or PS-NMM, reduces the amount of PS-Trisamine required in the reaction by removing the hydrogen chloride formed. In addition to acid chlorides, benzenesulfonyl chloride and 4-methoxyphenyl isocyanate were effectively scavenged in 0.5 h.
Part No: TN123.06Issued year: 2006File size: 0.07mbFile type: pdf
This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the
purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.