The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly abused drugs as recommended by The National Institute on Drug Abuse (NIDA). drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,

Amitriptyline Amphetamine Application Notes Benzoylecgonine Biological fluids Butylone Codeine Column Desipramine Forensic Imipramine Methamphetamine Morphine Nortryptiline Plasma RapidTrace Tricyclic Antidepressant Urine

Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid. Cyclosporine, Cyclosporine, Immunosuppressant,

Application Notes Clinical Column Cyclosporin Cyclosporine Immunosuppressant RapidTrace Whole blood

This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation. Opiates, drug testing, DOA, Ion exchange

Application Notes Codeine Column Forensic Morphine Opiates RapidTrace Urine

This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.

Application Notes Benzoylecgonine Cocaines Column Forensic RapidTrace Urine

The fermentation process associated with the manufacture of food grade enzyme powders was recently determined to support the colonization of bacteria. To control this issue, manufactures following different international regulatory procedures have found the addition of familiar antibiotic compounds to manufacturing processes beneficial. This becomes an issue of public health as the concentration of antibiotic residues in food grade materials promotes unknown exposure to the global community. Continued interest in the biomonitoring of these compounds have inspired a number of method development strategies; however, classic methods are labor intensive and require multi-step time consuming efforts. The FDA has recently mandated a method for the determination of chloramphenicol in shellfish. This study evaluates the feasibility of method modifications to incorporate enzyme powder matrices into this method. Linearity was determined over a range of 0.1 – 5 ng/mL (r^2 > 0.990). Relative recovery was determined >80%. The typical repeatability (%RSD) for n=7 replicates <20%. Strategies to control solids prior to SPE load will be presented as they were determined critical in method performance. Method robustness was determined by evaluating multiple lots and multiple variants of enzyme powder origin. The key variable is the nature of the excipients as high level sugars of different sources need to be controlled to minimize the ESI suppression.

Antibiotics Chloramphenicol Column Enzyme powder LC-MS/MS Posters

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. MSACL 2017, Palm Springs ASMS 2017, Indianapolis

96-well plate Biotin Clinical English LC-MS/MS Posters Serum Vitamin B7 Vitamins Water Soluble Vitamins

This poster describes a validated LC-MS/MS method for the quantification of nicotine, its metabolites: cotinine, trans-3’-hydroxycotinine, and the tobacco plant alkaloid anabasine in patient urine to determine their clinical utility for the evaluation of active smokers for pre-transplant eligibility. ASMS 2016 Disclaimer: Publication of this poster in no way implies or construes endorsement of Biotage products by Emory University School of Medicine.

3-OH-cotinine Anabasine Clinical Column Cotinine English LC-MS/MS Nicotine Nicotine metabolites Posters Urine

This poster compares various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of extraction techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression, phospholipid removal and simplicity of transfer to an automated sample preparation platform. ASMS 2016

96-well plate Acidic Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Posters Serum

The formation of 2,5-diketopiperazines from a water suspension of N-Boc protected dipeptide alkyl esters or dipeptide alkyl esters was induced by microwave assisted heating (Scheme 1). The products, which precipitated, were obtained in good yields. However, epimerisation/diastereomer formation was found to various extents depending on the amino acid sequence and the applied reaction temperature.

Posters

Introduction The tert-butyloxycarbonyl (Boc) group is a commonly used protecting group for amines in organic synthesis particularly in peptide synthesis.

The cyclic peptide mupain-1 (CPAYSRYLDC) is an inhibitor of the cancer-related urokinase-type plasminogen activator. Here we show the synthesis of a non-natural arginine building block precursor using MAOS.

Application Notes

Novel reactions Microwave Chemistry gives... Faster reactions Higher Yields Better purities Novel reactions

PNA oligomers have interesting properties such as their increased thermal and chemical stability and resistance to enzymatic degradation which drives their use in various molecular biology, molecular diagnostic, microarray, biosensor and antisense applications. In this study we synthesize two PNA oligomers on a small scale using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer.

Application Notes

Solid-Assisted Solution Phase Synthesis (SASPS) is a technique where the target molecule is synthesized in solution and solid-supported molecules are used as reagents, catalysts or scavengers. Recently this technique has been getting a lot of attention as an alternative to solid phase organic synthesis (SPOS), since it offers many of the advantages of SPOS in terms of ease of reaction workup and product purification in addition to the advantages associated with traditional solution-phase chemistry (e.g. the ease of monitoring the progress of the reaction by simply applying LC-MS or TLC techniques).

Product Notes

The application of microwave irradiation in solid phase peptide synthesis (SPPS), is an important tool for the synthesis of difficult and long peptide sequences that are often obtained in poor quality by comparable conventional methods at room temperature. Here we present the results obtained for the synthesis of a number of difficult peptide sequences using different levels of automation for microwave assisted SPPS: manual, semi-automated and fully automated systems. Syro wave, initiator, sp wave

Posters

Overview

Initiate success in Chemical education with Microwave Assisted Organic Synthesis. This leaflet contain example reactions that could be adapted for use in the teaching laboratory. Keywords: initiator+

English Manuals & User Guides Pharma

Migrate from mg to g Without Reoptimization. Biotage high precision reaction vials are engineered for durability and safe reactions. All vials withstand 30 bar pressures in a wide range of conditions. Designed for use with Biotage® Initiator+ and Initiator microwave synthesizers.

Product Notes

Speeding up reactions has never been easier. Biotage microwave synthesizers are the first-choice tools for organic chemists who need to accelerate their production of new compounds. Initiator, Initiator+, robot, vials, phase separator, work up tools

Brochures

The 60-position sample bed supports the production of focused libraries, multi-user environments and scale-out. Flexible operation enables the use of both large and small vials in combination at any time and in any order without manual intervention.

Product Notes

• Introduction • Carbonylations • Oxidative Heck reactions – Pd(II) • Heck reactions – Pd(0) • HIV-1 protease inhibitors • Acknowledgement

• Introduction • HIV-1 protease inhibitors – Shielded – Cyclic • Aqueous chemistry and Carbonylation reactions • Mycobacterium tuberculosis (TB) – Glutamine synthetase • Heck reactions • Acknowledgement • Extra material

Introduction Solid-phase synthesis combined with fast and precise heating by microwave irradiation has become a valuable tool for peptide chemists. It enables easier solid-phase assembly of difficult peptide sequences and even small proteins. Here we present, a study of microwave assisted solidphase peptide synthesis using different resins on the Syro WaveTM. Furthermore, we use this methodology to synthesize difficult peptide sequences including a multiple display antimicrobial peptide.

Posters

There is a constant need for new methodologies to produce focused libraries for unique compounds including protease inhibitors.

Heterocycle Synthesis • Quinolinones • Bisquinolones • Desulfitative C-C cross-couplings SiC Passive Heating Elements • Heating aids • Modulation of MW power (MW effects)

This paper evaluates solvent effect upon flash chromatography purification. The impact of sample mass overloading, volume overloading and sample dissolution solvents upon sample resolution are discussed.

MP-Borohydride is a macroporous, resinbound borohydride that is a resin bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones (Scheme 1). While the free form reagent is generally used in large excess and requires extraction of the reaction mixture for workup, the resin bound form can be used in slight excess and removed easily via filtration of the resin. The resin is compatible with microwave heating, which reduces the reaction time from hours to minutes4.

Product Notes

MP-TsO-TEMPO is a macroporous polystyrene-bound equivalent of oxoammonium p-toluenesulfonate. The resin has primary applications in the oxidation of activated primary and secondary alcohols to their corresponding aldehydes and ketones under mild reaction conditions.MP-TsO-TEMPO has been found to be highly selective for oxidation of allylic, benzylic and alicyclic hydroxy compounds.Oxidations with MP-TsO-TEMPO are very clean and the product is isolated by filtration of the resin followed by solvent removal.

MP-TsOH(65) columns have been designed specifically for isolation and purification of amines. The columns may also be used to scavenge basic reagents, substrates and reaction byproducts. A range of configurations is available from 100 mg/3 mL to 2.5 g/25 mL, including tab-less 3 and 6 mL for 48 and 24- well applications respectively. See page 6 for ordering information.

Product Notes

• Outline our plans to use catalysis in synthesis and our designs on the subject utilizing small, densely-functionalized building blocks • Discuss mechanistic issues surrounding regio- and chemoselectivity in oxidative addition with Pd in situations where a number of functional groups can be activated • Demonstrate the concepts put to practice in natural products synthesis • Discuss some of our catalysis work in the context of microwave-assisted organic synthesis (MAOS) and its application to library preparation • Outline directions that we are taking with library preparation utilizing a newly designed micro flow reactor system that is driven by microwave irradiation • Multi-component reactions (MCR) in micro channels using flow MAOS

The MultiSynTech Company develops accessories for peptide and combinatorial chemistry, as well as for automated synthesis. Our synthesis machines are used for parallel synthesis on solid phase as well as for solution chemistry. The multiple, fully automatic synthesis robot SYRO and the semi-automatic synthesis stations SAP, SAM and SAS are used in wide areas of organic chemistry, medicinal chemistry and also academic research for the synthesis of peptides and substance libraries for small molecules.

Brochures

Die Firma MultiSynTech entwickelt Hilfsmittel für die Peptid- und kombinatorische Chemie sowie für automatisierte Synthesen. Unsere Syntheseautomaten werden für die Parallelsynthese an fester Phase, wie auch für die Chemie in Lösung verwendet. Der multiple, vollautomatische Syntheseroboter Syro, die halbautomatischen Synthesestationen SAP, SAM und SAS werden in weiten Bereichen der organischen Chemie, der medizinischen Chemie wie auch in der Hochschulforschung für die Synthese von Peptiden und Substanzbibliotheken kleiner Moleküle eingesetzt. Für Anwendungen, die eine große Anzahl von Peptiden mit nur geringen Substanzmengen erfordern, haben wir die Tip-Synthese- und Spot-Synthese-Module entwickelt, die im Handumdrehen auf dem Syro installiert werden können. In Zusammenarbeit mit führenden Herstellern von Chemikalien haben wir eine Palette von Peptidreagenzien aufgebaut, die sowohl qualitativ als auch preislich für unsere Kunden Vorteile bietet.

Brochures German

This poster describes the optimization of a solid phase extraction method for the extraction of Ochratoxin A from red wine using ISOLUTE Myco SPE columns.

Column English Food LC-MS/MS Mycotoxin Ochratoxin A Posters Wine (red)

Isolera™ Dalton was used to fractionate closely running nicotinic extracts from commercially available tobacco leaves. Keywords: natural, products, normal, extraction, tobacco, nicotine, nornicotine

Application Notes English

Utilization of synthetic chemistry routes developed in late lead optimization is becoming more widespread as the desire to efficiently confirm pre-clinical compounds has increased. In some cases, chemical processes use reagents which are specific and cannot be substituted on scale up. Such reagents include transition metal catalysts.

English Posters

SNAP Ultra cartridges increase purification throughput and product purity compared to larger standard silica cartridges.

Posters

New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.

Posters

In this poster we will show how a new high-performance reversed-phase flash cartridge increases separation power and increases loading capacity 2x over conventional C18 cartridges.

Posters

Heterogeneous Catalysis Benefits: workup recycling environment economics Disadvantages: reaction rates

This poster demonstrates a simplified protocol for the simultaneous extraction of nicotine and its major metabolites including cotinine, nornicotine, 3-OH-cotinine, norcotinine and anabasine in plasma, urine, serum and whole blood using supported liquid extraction (SLE) followed by UPLC-MS/MS analysis. MSACL 2014

3-OH-cotinine 96-well plate Anabasine Clinical Cotinine English LC-MS/MS Nicotine Nicotine metabolites Norcotinine Plasma Posters Serum Urine Whole blood

This poster demonstrates a simplified protocol for the simultaneous extraction of nicotine and its major metabolites including cotinine, nornicotine, 3-OH-cotinine, norcotinine and anabasine in plasma, urine, serum and whole blood using supported liquid extraction (SLE) followed by UPLC-MS/MS analysis. Pittcon 2014

3-OH-cotinine 96-well plate Anabasine Clinical Cotinine English LC-MS/MS Nicotine Nicotine metabolites Norcotinine Nornicotine Plasma Posters Serum Urine

A major cost for synthetic chemistry laboratories is solvent, especially solvent used for compound purification. Much of the wasted solvent lies in the space between peaks as seen in figure 1. As companies look to decrease operating costs, this expense is being targeted and ways to reduce expenditures sought.

Application Notes English

The formation of diarylamines by reacting anilines with aromatic electrophiles is, usually, a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield, due to the low nucleophilicity of anilines.1 The Buchwald-Hartwig cross-coupling reaction is a very useable and elegant solution to this problem.2,3,4 Anticipating the classical metal-free SNAr-amination reaction, without using Pd-catalysts and strong bases, with only higher temperature and pressure as tool as depicted in the Scheme below,1 gives no product at all at 180 ºC and very low conversion (9 %) at 220 ºC for 15 minutes when heating by microwaves.5 Remainder was unreacted starting material.

Application Notes English

Horizon Technology products revolve around making oil and grease analyses and semivolatile organic extractions safer, faster, more accurate and reproducible. Best of all, with reduced solvent usage, costs are reduced! Coupled with rugged hardware, applications and support, your lab can be more productive than ever before.

Product Notes

A one-pot process for the preparation of Acyclovir from guanine is described. The key step involves a trimethylsilyl triflate (TfOTMS)-catalysed reaction of silylated guanine with 1,3-dioxalane under microwave irradiation. With this method, the yield is in the same range as with conventional heating, but the speed of the reaction is increased by a factor of 85.

Application Notes

Hair is a useful matrix for drug analysis, as it can be used to show historical drug use. Parent drugs are often present in high concentrations, and the matrix is stable over time. This poster describes the use of the Biotage® Lysera to pulverise and extract hair samples, with ISOLUTE® SLE+ supported liquid extraction columns to clean up the samples prior to analysis. Limits of quantitation set by the Society of Hair Testing for amphetamines are achievable with the described method (0.2 ng/mg). SoHT 2017, Cardiff UK

Amphetamine Amphetamines Bead Ruptor Column Drugs of Abuse English Forensic GC-MS Hair MDA MDEA MDMA Methamphetamine Posters

Scale-up projects carried out by the Discovery Process Research (DPR) group at Dow AgroSciences must produce sizeable field samples of reaction products originally developed by Dow's discovery group

Customer Cases

This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.

2,4,6-trichlorophenol 2,4-D 2,4-dichlorophenol 2,4-dimethylphenol 2,4-dinitrophenol 2,6-dichlorophenol 2,6-dinitro-2-methylphenol 2-methylphenol 2-nitrophenol 3-methylphenol 4-chloro-3-methylphenol 4-methylphenol 4-nitrophenol Acenapthalene Acid herbicides Ametrine Anthracene Application Notes Arachlor Atrazine Base-neutral herbicides Bentazone Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Desethyl atrazine Desisopropyl atrazine Dibenzo(ah)anthracene Dibenzylbutyl phthalate Dibutyl phthalate Diethyl phthalate Dimethyl phthalate Dioctyl phthalate English Environmental Environmental contaminants Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Indeno(123-cd)pyrene LC-MS/MS MCPA MCPB Metribuzin Molinate Organochlorine pesticides (OCP) Organophosphate Pesticide Organophosphates PAHs Persistant organic pollutants (POP) Pesticides Phenanthrene Phenols Phthalates Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Product Notes Pyrene Water

For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.

Posters