The formation of diarylamines by reacting anilines with aromatic electrophiles is, usually, a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield, due to the low nucleophilicity of anilines.1 The Buchwald-Hartwig cross-coupling reaction is a very useable and elegant solution to this problem.2,3,4 Anticipating the classical metal-free SNAr-amination reaction, without using Pd-catalysts and strong bases, with only higher temperature and pressure as tool as depicted in the Scheme below,1 gives no product at all at 180 ºC and very low conversion (9 %) at 220 ºC for 15 minutes when heating by microwaves.5 Remainder was unreacted starting material.

Application Notes English

Horizon Technology products revolve around making oil and grease analyses and semivolatile organic extractions safer, faster, more accurate and reproducible. Best of all, with reduced solvent usage, costs are reduced! Coupled with rugged hardware, applications and support, your lab can be more productive than ever before.

Product Notes

A one-pot process for the preparation of Acyclovir from guanine is described. The key step involves a trimethylsilyl triflate (TfOTMS)-catalysed reaction of silylated guanine with 1,3-dioxalane under microwave irradiation. With this method, the yield is in the same range as with conventional heating, but the speed of the reaction is increased by a factor of 85.

Application Notes

Hair is a useful matrix for drug analysis, as it can be used to show historical drug use. Parent drugs are often present in high concentrations, and the matrix is stable over time. This poster describes the use of the Biotage® Lysera to pulverise and extract hair samples, with ISOLUTE® SLE+ supported liquid extraction columns to clean up the samples prior to analysis. Limits of quantitation set by the Society of Hair Testing for amphetamines are achievable with the described method (0.2 ng/mg). SoHT 2017, Cardiff UK

Amphetamine Amphetamines Bead Ruptor Column Drugs of Abuse English Forensic GC-MS Hair MDA MDEA MDMA Methamphetamine Posters

Scale-up projects carried out by the Discovery Process Research (DPR) group at Dow AgroSciences must produce sizeable field samples of reaction products originally developed by Dow's discovery group

Customer Cases

This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.

2,4,6-trichlorophenol 2,4-D 2,4-dichlorophenol 2,4-dimethylphenol 2,4-dinitrophenol 2,6-dichlorophenol 2,6-dinitro-2-methylphenol 2-methylphenol 2-nitrophenol 3-methylphenol 4-chloro-3-methylphenol 4-methylphenol 4-nitrophenol Acenapthalene Acid herbicides Ametrine Anthracene Application Notes Arachlor Atrazine Base-neutral herbicides Bentazone Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(ghi)perylene Benzo(k)Fluoranthene Chrysene Column Desethyl atrazine Desisopropyl atrazine Dibenzo(ah)anthracene Dibenzylbutyl phthalate Dibutyl phthalate Diethyl phthalate Dimethyl phthalate Dioctyl phthalate English Environmental Environmental contaminants Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Indeno(123-cd)pyrene LC-MS/MS MCPA MCPB Metribuzin Molinate Organochlorine pesticides (OCP) Organophosphate Pesticide Organophosphates PAHs Persistant organic pollutants (POP) Pesticides Phenanthrene Phenols Phthalates Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Product Notes Pyrene Water

For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.

Posters

The results from this research show that the chromatographic purification of organic amine compounds can be improved simply by using an amine-functionalized silica and typical normal-phase solvents such as hexane and ethyl acetate.

Posters

Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.

Brochures

PAL-ChemMatrix is highly recommended for microwave synthesis. Under microwave heating conditions, such as Biotage Initiator the PAL-ChemMatrix provides higher purity peptides than the Rink Amide ChemMatrix. For manual synthesis or non-microwave synthesis, we recommend using the Rink Amide ChemMatrix resin.

Product Notes

A series of eight 3-alkylidylindolin-2-ones were conveniently synthesized on Biotage® Endeavor™ in high yield and purity. Reactor purging, agitation, pressurization, heating, gas uptake and run termination were performed automatically based on the run program.

Application Notes

How to uninstall Java 7 and install Java 6 to get Pathfinder working

Software

Application Notes

Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. These solutions make your workflow more efficient and improve the quality of peptides you synthesize. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.

Brochures

Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where they synthesize complex molecules for chemical biology applications. An important tool that contributes to their success has been Biotage® Initiator+ Alstra™ microwave peptide synthesizer, and they are even breaking the dogma that only HPLC can be used for peptide purification, by using cutting edge reversed phase flash purification techniques provided by Biotage.

Customer Cases

Biotage ZIP flash chromatography cartridges are engineered to meet the needs of chemists requiring high performance and consistency from a purification cartridge at a value price to reduce purification costs. Isolera Prime flash purification system.

Product Notes

Application Notes

Application Notes

Application Notes

Sample preparation is an essential technique prior to LC-MS/MS analysis of drugs in biological fluid samples to remove unwanted matrix components. These matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster investigates the performance of EVOLUTE™ CX for overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.

Posters

PS-HOBt(HL) is a higher loading version (0.9 – 1.4 mmol/g) of a sulfonamide-linked, resin-bound equivalent of 1-hydroxybenzotriazole (HOBt). PS-HOBt(HL) can be used as an effective activating reagent for transfer of protecting groups, e.g., Fmoc and Cbz, to amines.

PS-TBD is a polymer-supported base, which consists of a bicyclic guanidine moiety (1,5,7- triazabicyclo[4.4.0]dec-5-ene) anchored on polystyrene. PS-TBD is a stronger polymersupported base than the PS-DIEA and PS-NMM resins, which are bound tertiary amines that are used in organic synthesis and reagent scavenging. Published applications of PS-TBD include alkylation of phenolsi and amines;ii esterification of carboxylic acids using alkyl halides; alkylation of activated methylene compounds; de-halogenation of organic halides;iii high throughput synthesis of aryl triflates and aryl nonaflates,iv and the regioselective synthesis of lysophospholipids.

Product Notes

PS-DEAM is a polystyrene-bound diethanolamine, which is a resin-bound equivalent of the tridentate (N, O, O) N-alkyldiethanolamine ligand. PS-DEAM is an effective scavenger for a variety of oxophilic organometallic and inorganic complexes. The resin can be used to quench reactions and remove metallic reagents, catalysts or byproducts, allowing the purified product to be isolated by filtration. Boronic acids are scavenged by PS-DEAM. This has been exploited in the purification of Suzuki reactions through the removal of excess boronic acid and coupling byproducts.

Product Notes

MP-Borohydride is a macroporous, polystyrene-bound borohydride, that is a resin-bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones. The resin, in conjunction with some transition metal salts, can also be used for a number of other important reductive applications, such as reduction of oximes, azides and alkyl halides. The reduced products are isolated by simple filtration away from the resin.

Product Notes

This poster discusses the effect of various sample preparation protocols of the recovery of a range of different Drugs of abuse classes, extracted from oral fluid collected using the Quantisal collection device. MSACL NA 2018, Palm Springs CA ASMS 2018, San Diego, CA

Anaesthetic Anticonvulsant Benzodiazepines Cannabinoid Carbamates Clinical Column English Forensic LC-MS/MS Opioid Oral fluid Posters Saliva Stimulants Tricyclic Antidepressant z-drugs

Oral fluid is of increasing importance as a biological matrix in drugs of abuse and related analyte testing because of the ease of sample collection, and difficulty in adulteration of samples, particularly when compared with urine.

Application Notes Forensic

The automated Advantage Series™ 3400 ChemCorder™ workflow replicator was used to record all the manual operations a chemist performs during a highly exothermic chemical reaction (Figure 1).Before the experiment was initiated, the ChemCorder replicator was put in record mode.All steps of the experiment were accurately recorded, including any comments and observations. Once completed the ChemCorder replicator produced a report and recipe file of the chemistry. This recipe file was used to accurately and automatically repeat the reaction. Alternatively, the file could have been modified to produce a new recipe based on the recorded chemistry

Application Notes

English Manuals & User Guides

English Manuals & User Guides

PRESSURE+ Declaration of Conformity

Certificates

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.

Product Notes

Product Notes

Product Notes

PS-DMAP is a polymer-bound equivalent of dimethylaminopyridine (DMAP) which may be used as a catalyst for acylation and related reactions. PS-DMAP has a loading of 1.4 mmol/g based on nitrogen analysis. Typical catalysis conditions require 10 - 20 mol% relative to the nucleophile. Catalytic PS-DMAP accelerates the acylation of sluggish nucleophiles, e.g. tertiary alcohols.

Product Notes

Product Notes

Product Notes

PS-TBD (Figure 1) is a polystyrene bound guanidine base, which is stronger than corresponding resin bound DIEA (DIPEA) and bound N-Methyl-morpholine resins. It has a wide variety of uses in synthesis, from direct alkylation through to sequestering of acidic components of reaction.

An oxidation method using a novel solid phase oxidant, based on a covalently polystyrene bound, sulfonic ester linked 2,2,6,6-tetramethylpiperidine-1-oxyl species is presented, PS-TEMPO (Fig.1a&1b).1,2 The reagent showed fast room temperature kinetics compared to similar known systems3 when used to oxidize primary and secondary alcohols to aldehydes and ketones, respectively (Table 1). The products were in general of high purity and the isolated yields were good to excellent. The used reagent could be reactivated and reused for multiple oxidation reactions. The colour change observed as the oxidation state of the reagent changed, gave an indication of the extent to which the reagent had been used.

Posters

PS-Thiophenol is based on an aminomethyl resin with a tethered thiophenol functionality. The resin was designed for the scavenging of alkylating agents, e.g. alkyl halides. PS-Thiophenol was tested and found effective in scavenging alkylating agents ranging from octyl bromide to benzyl bromide. The scavenging effectiveness of PS-Thiophenol was found to be greater than a polymer-bound benzyl thiol towards octyl bromide, indicative of the higher nucleophilicity of the thiophenolate.

PS-Triphenylphosphine is a resin-bound equivalent of triphenylphosphine. The capacity of the resin is determined by the quantitation of benzyl bromide uptake in DMF (GC, internal standard method).

Product Notes

PS-Trisamine is an amine functional resin for the removal of excess electrophilic reagents during the quenching and purification of reaction mixtures.1 PS-Trisamine resin has a scavenging capacity of 3.0–4.0 mmol/g based on reaction with an excess of benzoyl chloride. Scavenging of common electrophiles typically requires 3–6 equiv of PS-Trisamine and occurs within 0.5–3 h at room temperature. The scope of PS-Trisamine as a scavenger for electrophilic reagents was investigated using 4-chlorobenzoyl chloride, 2-phenylbutyryl chloride and 2,6-dimethoxybenzoyl chloride as a series of acid chlorides with decreasing reactivity.Acid chlorides were completely scavenged in 0.5 h using 3.5 equiv PS-Trisamine (Table 1). The presence of a tertiary base, e.g. PS-DIEA or PS-NMM, reduces the amount of PS-Trisamine required in the reaction by removing the hydrogen chloride formed. In addition to acid chlorides, benzenesulfonyl chloride and 4-methoxyphenyl isocyanate were effectively scavenged in 0.5 h.

Product Notes

This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.

Application Notes Product Notes

Peptides are naturally occurring polyamides, derived from 20 amino acids.

Application Notes

Information on how to navigate in the software and hints and tips on how to prepare reagents for peptide synthesis and circumvent side reactions.

English Manuals & User Guides

Preparation, How Much are You Purifying?

Manuals & User Guides