The automated Advantage Series™ 3400 ChemCorder™ workflow replicator was used to record all the manual operations a chemist performs during a highly exothermic chemical reaction (Figure 1).Before the experiment was initiated, the ChemCorder replicator was put in record mode.All steps of the experiment were accurately recorded, including any comments and observations. Once completed the ChemCorder replicator produced a report and recipe file of the chemistry. This recipe file was used to accurately and automatically repeat the reaction. Alternatively, the file could have been modified to produce a new recipe based on the recorded chemistry

Application Notes

English Manuals & User Guides

English Manuals & User Guides

PRESSURE+ Declaration of Conformity

Certificates

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.

Product Notes

Product Notes

Product Notes

PS-DMAP is a polymer-bound equivalent of dimethylaminopyridine (DMAP) which may be used as a catalyst for acylation and related reactions. PS-DMAP has a loading of 1.4 mmol/g based on nitrogen analysis. Typical catalysis conditions require 10 - 20 mol% relative to the nucleophile. Catalytic PS-DMAP accelerates the acylation of sluggish nucleophiles, e.g. tertiary alcohols.

Product Notes

Product Notes

Product Notes

PS-TBD (Figure 1) is a polystyrene bound guanidine base, which is stronger than corresponding resin bound DIEA (DIPEA) and bound N-Methyl-morpholine resins. It has a wide variety of uses in synthesis, from direct alkylation through to sequestering of acidic components of reaction.

An oxidation method using a novel solid phase oxidant, based on a covalently polystyrene bound, sulfonic ester linked 2,2,6,6-tetramethylpiperidine-1-oxyl species is presented, PS-TEMPO (Fig.1a&1b).1,2 The reagent showed fast room temperature kinetics compared to similar known systems3 when used to oxidize primary and secondary alcohols to aldehydes and ketones, respectively (Table 1). The products were in general of high purity and the isolated yields were good to excellent. The used reagent could be reactivated and reused for multiple oxidation reactions. The colour change observed as the oxidation state of the reagent changed, gave an indication of the extent to which the reagent had been used.

Posters

PS-Thiophenol is based on an aminomethyl resin with a tethered thiophenol functionality. The resin was designed for the scavenging of alkylating agents, e.g. alkyl halides. PS-Thiophenol was tested and found effective in scavenging alkylating agents ranging from octyl bromide to benzyl bromide. The scavenging effectiveness of PS-Thiophenol was found to be greater than a polymer-bound benzyl thiol towards octyl bromide, indicative of the higher nucleophilicity of the thiophenolate.

PS-Triphenylphosphine is a resin-bound equivalent of triphenylphosphine. The capacity of the resin is determined by the quantitation of benzyl bromide uptake in DMF (GC, internal standard method).

Product Notes

PS-Trisamine is an amine functional resin for the removal of excess electrophilic reagents during the quenching and purification of reaction mixtures.1 PS-Trisamine resin has a scavenging capacity of 3.0–4.0 mmol/g based on reaction with an excess of benzoyl chloride. Scavenging of common electrophiles typically requires 3–6 equiv of PS-Trisamine and occurs within 0.5–3 h at room temperature. The scope of PS-Trisamine as a scavenger for electrophilic reagents was investigated using 4-chlorobenzoyl chloride, 2-phenylbutyryl chloride and 2,6-dimethoxybenzoyl chloride as a series of acid chlorides with decreasing reactivity.Acid chlorides were completely scavenged in 0.5 h using 3.5 equiv PS-Trisamine (Table 1). The presence of a tertiary base, e.g. PS-DIEA or PS-NMM, reduces the amount of PS-Trisamine required in the reaction by removing the hydrogen chloride formed. In addition to acid chlorides, benzenesulfonyl chloride and 4-methoxyphenyl isocyanate were effectively scavenged in 0.5 h.

Product Notes

This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.

Application Notes Product Notes

Peptides are naturally occurring polyamides, derived from 20 amino acids.

Application Notes

Information on how to navigate in the software and hints and tips on how to prepare reagents for peptide synthesis and circumvent side reactions.

English Manuals & User Guides

Preparation, How Much are You Purifying?

Manuals & User Guides

Create a Gradient for One or Multiple Compounds, Optimizing Scale-Up, Transferring Isolera Methods between Systems, Upload Method on another Isolera.

Manuals & User Guides

Manuals & User Guides

Manuals & User Guides

Manuals & User Guides

QuickStart Guide to SPE

English Manuals & User Guides

We have successfully developed a rapid and efficient microwave-assisted protocol for the preparation of Neu5Ac congeners that are important intermediates for clinically-relevant derivatives of Neu5Ac. The syntheses were performed using readily available reagents, improved on established techniques, and used more benign reagent for peracetylation. Moreover, the protocol that we developed is 1 to 2 orders of magnitude faster than conventional methods. Initiator+ SPWave synthesis system Dhananjaya Sahoo, I. Abrrey Monreal, and Jonel P. Saludes

English Posters

For high efficiencyin parallel synthesisand method developement short reaction timesare essential. We have examinedthe possibilityof maintaining reported selectivities[1]in rapid, high temperaturepalladium-catalyzed asymmetric allylic alkylations. Reactions were promotedby useof singlemode microwave heating(vide infra),[2]which oppositeto conventional oilbath heating(wallheat transfer) rapidlyand almost homogeneouslyheat largeparts of the sample(internalheat transfer).

Posters

Topics •Microwave-assisted organic synthesis –Advantages –Scope •Solid supported reagents and scavengers –Advantages in solution phase synthesis •Key Transformations using solid-supported reagents/scavengers AmidationAryl-aryl bond formation (Suzuki Reaction)AlkylationOxidationReductive aminationAcid catalyzed Reactions Base Catalyzed reaction

Crystallization affects every aspect of process chemistry including process transfers, filtration impurity purging drying, and particle size measurements.

Customer Cases

This Chemistry Data Sheet describes the use of ISOLUTE Phase Separator columns and 96-well plates to separate aqueous solutions from chlorinated organic solvents.

96-well plate Application Notes English Manuals & User Guides Product Notes

Introduction •Metal-mediated microwave-heated organic reactions –Instant chemistry –Carbonylations –Aminoindans –AT2selectivepeptidomimetics –Heck reactions –Plasmepsininhibitors –Pulsed microwave irradiation -PCR •Acknowledgement •Extra material

User report: RapidTrace. The Laboratory of Racing Chemistry (LRC) analyzes samples from racehorses for doping drug. In order to screen approximately 1000 samples per week, LRC use an array of Biotage RapidTrace automated SPE systems. In an interview, Mr. Kenji Kinoshita and Mr. Kensaku Shirai talked about their experiences with the RapidTrace system in processing the heavy workload.

Customer Cases

This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system. EPH, Massachusetts Department of Environmental Protection,

Aliphatic hydrocarbos Application Notes Column Environmental Environmental contaminants Polyaromatic hydrocarbons RapidTrace Soil

The RapidTrace+ is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated pharmaceutical, clinical and forensic laboratories. The RapidTrace+ eliminates SPE bottlenecks so you can realize the full benefits of today’s powerful analytical instruments.

Brochures

English Manuals & User Guides RapidTrace

A multistep solution phase synthesis of a series of 5-benzofuranylmethyl-2,4-thiazolidine-diones was performed using an Endeavor catalyst screening system.

Application Notes

Pure fractions are in high demand – impurities mean more work after purification. With new technology, fraction purity can be digitally analyzed directly during chromatography to reveal any problems on the fly. In this application we will show how the Isolera Spektra is used to determine fraction purity, eliminating the need for other post-purification analysis.

Application Notes English

Our TurboVap II customers have been successful obtaining excellent recoveries for all semivolatile organic compounds (BNA, PAH, PCB, Pesticides, TPH/DRO, Herbicides). There are many factors that can impact the recoveries, such as client sample matrix, temperature, humidity, and extraction solvents. After gathering information from our customers, we have developed the guidelines below that will help your laboratory determine its optimum conditions for the TurboVap II.

Manuals & User Guides

Synthetic oligosaccharides and glycoconjugates provide materials for correlating structure with function. Synthetic mimics of the complex assemblies found on cell surfaces can modulate cellular interactions and are under development as therapeutic agents.

Drug analysis

We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved. EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.

96-well plate Biomarker Clinical English Histamine LC-MS/MS Mouse plasma Pharma Posters Serotonin

One very common transformation in organic synthesis is the reduction of aldehydes or ketones to the corresponding primary or secondary alcohols (Scheme 1) using sodium borohydride as hydride donator. This reaction generally generates the product in very high yield. Among the draw-backs are long reaction times, that a large excess of reagent is needed and that the reaction mixture must be extracted in order to separate the inorganic salts from the product.

Posters

In this example, Isolera™ Dalton was used to fractionate completely non-UV absorbing starting materials and products from a classic reductive amination reaction. A resin bound cyanborohydride reagent was used to greatly facilitate the work flow by eliminating the need for traditional work-up, and the mixture applied to flash chromatography purification methods. Isolera, Dalton

Application Notes English

Reductive amination is a very powerful chemical transformation, but the outcome from heating this reaction with microwaves is very unpredictable.

Posters

This application note demonstrates the efficient and selective removal of a wide range of pesticides from natural lemon oil using RENSA® 101.

Application Notes

MIP Technologies AB has developed a new resin for silver ion chromatography, RENSA® 101. The surface of the material contains hydrophobic moieties along with silver ions bound to a sulfonic acid functionality.

Product Notes

The designed resin RENSA® 103 is a novel type of strong cation exchanger. Unlike most conventional ion exchangers, the material is characterized by a high cross-linking degree and an alternative route of incorporation of the ionic moieties. MIP

Product Notes

MIP Technologies AB (a subsidiary of Biotage AB) has developed a new type of affinity resin, RENSA® Boronate (Figure 1) with selective boronic acid surface functional groups. It has an optimized distribution and accessibility of hydrophobic (phenyl) and selective boronic acid moieties (Figure 2). It is produced by a proprietary particle synthesis technology and exhibits a material architecture and pore size distribution that is optimized for binding of small and oligomeric molecules.

Product Notes