PS-TBD is a polymer-supported base, which consists of a bicyclic guanidine moiety (1,5,7- triazabicyclo[4.4.0]dec-5-ene) anchored on polystyrene. PS-TBD is a stronger polymersupported base than the PS-DIEA and PS-NMM resins, which are bound tertiary amines that are used in organic synthesis and reagent scavenging. Published applications of PS-TBD include alkylation of phenolsi and amines;ii esterification of carboxylic acids using alkyl halides; alkylation of activated methylene compounds; de-halogenation of organic halides;iii high throughput synthesis of aryl triflates and aryl nonaflates,iv and the regioselective synthesis of lysophospholipids.

Product Notes

PS-DEAM is a polystyrene-bound diethanolamine, which is a resin-bound equivalent of the tridentate (N, O, O) N-alkyldiethanolamine ligand. PS-DEAM is an effective scavenger for a variety of oxophilic organometallic and inorganic complexes. The resin can be used to quench reactions and remove metallic reagents, catalysts or byproducts, allowing the purified product to be isolated by filtration. Boronic acids are scavenged by PS-DEAM. This has been exploited in the purification of Suzuki reactions through the removal of excess boronic acid and coupling byproducts.

Product Notes

MP-Borohydride is a macroporous, polystyrene-bound borohydride, that is a resin-bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones. The resin, in conjunction with some transition metal salts, can also be used for a number of other important reductive applications, such as reduction of oximes, azides and alkyl halides. The reduced products are isolated by simple filtration away from the resin.

Product Notes

This poster discusses the effect of various sample preparation protocols of the recovery of a range of different Drugs of abuse classes, extracted from oral fluid collected using the Quantisal collection device. MSACL NA 2018, Palm Springs CA

Anaesthetic Anticonvulsant Benzodiazepines Cannabinoid Carbamates Clinical Column English Forensic LC-MS/MS Opioid Oral fluid Posters Saliva Stimulants Tricyclic Antidepressant z-drugs

The automated Advantage Series™ 3400 ChemCorder™ workflow replicator was used to record all the manual operations a chemist performs during a highly exothermic chemical reaction (Figure 1).Before the experiment was initiated, the ChemCorder replicator was put in record mode.All steps of the experiment were accurately recorded, including any comments and observations. Once completed the ChemCorder replicator produced a report and recipe file of the chemistry. This recipe file was used to accurately and automatically repeat the reaction. Alternatively, the file could have been modified to produce a new recipe based on the recorded chemistry

Application Notes

English Manuals & User Guides

English Manuals & User Guides

PRESSURE+ Declaration of Conformity

Certificates

Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.

Product Notes

Product Notes

Product Notes

PS-DMAP is a polymer-bound equivalent of dimethylaminopyridine (DMAP) which may be used as a catalyst for acylation and related reactions. PS-DMAP has a loading of 1.4 mmol/g based on nitrogen analysis. Typical catalysis conditions require 10 - 20 mol% relative to the nucleophile. Catalytic PS-DMAP accelerates the acylation of sluggish nucleophiles, e.g. tertiary alcohols.

Product Notes

Product Notes

Product Notes

PS-TBD (Figure 1) is a polystyrene bound guanidine base, which is stronger than corresponding resin bound DIEA (DIPEA) and bound N-Methyl-morpholine resins. It has a wide variety of uses in synthesis, from direct alkylation through to sequestering of acidic components of reaction.

An oxidation method using a novel solid phase oxidant, based on a covalently polystyrene bound, sulfonic ester linked 2,2,6,6-tetramethylpiperidine-1-oxyl species is presented, PS-TEMPO (Fig.1a&1b).1,2 The reagent showed fast room temperature kinetics compared to similar known systems3 when used to oxidize primary and secondary alcohols to aldehydes and ketones, respectively (Table 1). The products were in general of high purity and the isolated yields were good to excellent. The used reagent could be reactivated and reused for multiple oxidation reactions. The colour change observed as the oxidation state of the reagent changed, gave an indication of the extent to which the reagent had been used.

Posters

PS-Thiophenol is based on an aminomethyl resin with a tethered thiophenol functionality. The resin was designed for the scavenging of alkylating agents, e.g. alkyl halides. PS-Thiophenol was tested and found effective in scavenging alkylating agents ranging from octyl bromide to benzyl bromide. The scavenging effectiveness of PS-Thiophenol was found to be greater than a polymer-bound benzyl thiol towards octyl bromide, indicative of the higher nucleophilicity of the thiophenolate.

PS-Triphenylphosphine is a resin-bound equivalent of triphenylphosphine. The capacity of the resin is determined by the quantitation of benzyl bromide uptake in DMF (GC, internal standard method).

Product Notes

PS-Trisamine is an amine functional resin for the removal of excess electrophilic reagents during the quenching and purification of reaction mixtures.1 PS-Trisamine resin has a scavenging capacity of 3.0–4.0 mmol/g based on reaction with an excess of benzoyl chloride. Scavenging of common electrophiles typically requires 3–6 equiv of PS-Trisamine and occurs within 0.5–3 h at room temperature. The scope of PS-Trisamine as a scavenger for electrophilic reagents was investigated using 4-chlorobenzoyl chloride, 2-phenylbutyryl chloride and 2,6-dimethoxybenzoyl chloride as a series of acid chlorides with decreasing reactivity.Acid chlorides were completely scavenged in 0.5 h using 3.5 equiv PS-Trisamine (Table 1). The presence of a tertiary base, e.g. PS-DIEA or PS-NMM, reduces the amount of PS-Trisamine required in the reaction by removing the hydrogen chloride formed. In addition to acid chlorides, benzenesulfonyl chloride and 4-methoxyphenyl isocyanate were effectively scavenged in 0.5 h.

Product Notes

This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.

Application Notes Product Notes

Peptides are naturally occurring polyamides, derived from 20 amino acids.

Application Notes

Information on how to navigate in the software and hints and tips on how to prepare reagents for peptide synthesis and circumvent side reactions.

English Manuals & User Guides

Preparation, How Much are You Purifying?

Manuals & User Guides

Create a Gradient for One or Multiple Compounds, Optimizing Scale-Up, Transferring Isolera Methods between Systems, Upload Method on another Isolera.

Manuals & User Guides

Manuals & User Guides

Manuals & User Guides

Manuals & User Guides

QuickStart Guide to SPE

English Manuals & User Guides

We have successfully developed a rapid and efficient microwave-assisted protocol for the preparation of Neu5Ac congeners that are important intermediates for clinically-relevant derivatives of Neu5Ac. The syntheses were performed using readily available reagents, improved on established techniques, and used more benign reagent for peracetylation. Moreover, the protocol that we developed is 1 to 2 orders of magnitude faster than conventional methods. Initiator+ SPWave synthesis system Dhananjaya Sahoo, I. Abrrey Monreal, and Jonel P. Saludes

English Posters

For high efficiencyin parallel synthesisand method developement short reaction timesare essential. We have examinedthe possibilityof maintaining reported selectivities[1]in rapid, high temperaturepalladium-catalyzed asymmetric allylic alkylations. Reactions were promotedby useof singlemode microwave heating(vide infra),[2]which oppositeto conventional oilbath heating(wallheat transfer) rapidlyand almost homogeneouslyheat largeparts of the sample(internalheat transfer).

Posters

Topics •Microwave-assisted organic synthesis –Advantages –Scope •Solid supported reagents and scavengers –Advantages in solution phase synthesis •Key Transformations using solid-supported reagents/scavengers AmidationAryl-aryl bond formation (Suzuki Reaction)AlkylationOxidationReductive aminationAcid catalyzed Reactions Base Catalyzed reaction

Crystallization affects every aspect of process chemistry including process transfers, filtration impurity purging drying, and particle size measurements.

Customer Cases

This Chemistry Data Sheet describes the use of ISOLUTE Phase Separator columns and 96-well plates to separate aqueous solutions from chlorinated organic solvents.

96-well plate Application Notes English Manuals & User Guides Product Notes

Introduction •Metal-mediated microwave-heated organic reactions –Instant chemistry –Carbonylations –Aminoindans –AT2selectivepeptidomimetics –Heck reactions –Plasmepsininhibitors –Pulsed microwave irradiation -PCR •Acknowledgement •Extra material

User report: RapidTrace. The Laboratory of Racing Chemistry (LRC) analyzes samples from racehorses for doping drug. In order to screen approximately 1000 samples per week, LRC use an array of Biotage RapidTrace automated SPE systems. In an interview, Mr. Kenji Kinoshita and Mr. Kensaku Shirai talked about their experiences with the RapidTrace system in processing the heavy workload.

Customer Cases

This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system. EPH, Massachusetts Department of Environmental Protection,

Aliphatic hydrocarbos Application Notes Column Environmental Environmental contaminants Polyaromatic hydrocarbons RapidTrace Soil

The RapidTrace+ is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated pharmaceutical, clinical and forensic laboratories. The RapidTrace+ eliminates SPE bottlenecks so you can realize the full benefits of today’s powerful analytical instruments.

Brochures

English Manuals & User Guides RapidTrace

A multistep solution phase synthesis of a series of 5-benzofuranylmethyl-2,4-thiazolidine-diones was performed using an Endeavor catalyst screening system.

Application Notes

Pure fractions are in high demand – impurities mean more work after purification. With new technology, fraction purity can be digitally analyzed directly during chromatography to reveal any problems on the fly. In this application we will show how the Isolera Spektra is used to determine fraction purity, eliminating the need for other post-purification analysis.

Application Notes English

Our TurboVap II customers have been successful obtaining excellent recoveries for all semivolatile organic compounds (BNA, PAH, PCB, Pesticides, TPH/DRO, Herbicides). There are many factors that can impact the recoveries, such as client sample matrix, temperature, humidity, and extraction solvents. After gathering information from our customers, we have developed the guidelines below that will help your laboratory determine its optimum conditions for the TurboVap II.

Manuals & User Guides

Synthetic oligosaccharides and glycoconjugates provide materials for correlating structure with function. Synthetic mimics of the complex assemblies found on cell surfaces can modulate cellular interactions and are under development as therapeutic agents.

Drug analysis

We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved. EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.

96-well plate Biomarker Clinical English Histamine LC-MS/MS Mouse plasma Pharma Posters Serotonin

One very common transformation in organic synthesis is the reduction of aldehydes or ketones to the corresponding primary or secondary alcohols (Scheme 1) using sodium borohydride as hydride donator. This reaction generally generates the product in very high yield. Among the draw-backs are long reaction times, that a large excess of reagent is needed and that the reaction mixture must be extracted in order to separate the inorganic salts from the product.

Posters

In this example, Isolera™ Dalton was used to fractionate completely non-UV absorbing starting materials and products from a classic reductive amination reaction. A resin bound cyanborohydride reagent was used to greatly facilitate the work flow by eliminating the need for traditional work-up, and the mixture applied to flash chromatography purification methods. Isolera, Dalton

Application Notes English