Purifying peptides from crude synthetic mixtures can be extremely challenging. The peptides often need to be separated from side products, (deletion and truncated sequences generated during the synthesis) some of which may possess nearly identical sequences. Biotage has a long history in traditional chromatographic separation. Biotage now offers Resolux reversed-phase HPLC columns as an ideal solution for separation of synthetic peptides. The Resolux HPLC columns provide excellent resolution and are an economical choice for the peptide chemist.
Part No: AN065Issued year: 2012File size: 0.99mbFile type: pdf
Reversed-phase chromatography facilitates the isolation of milligram to multi-gram quantities of polar compounds from naturally occurring materials or from synthetic reaction
products. Biotage reversed phase KP-C18-HS SNAP cartridges have been shown in a number of applications to be effective for the purification of natural products. The methodology is robust and may
be applied generically to new extraction purification runs.
Keywords: natural, products, reversed, phase, normal, extraction, spinach.
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Part No: Issued year: 2008File size: 0.14mbFile type: pdf
•Select mixed mode SPE based on functional groups present on molecule
–For basic amine containing molecules select MCX
–For molecules possessing a carboxyl group select MAX
–Easier to debug a failed extraction: pH control problem most common cause
Part No: CM-RINK-0810Issued year: 2010File size: 0.26mbFile type: pdf
Rink Amide-ChemMatrix is one of the most popular resins for for
both manual SPPS and automated SPPS, under microwave or
non-microwave conditions. For microwave synthesis, we also
recommend the use of PAL-ChemMatrix resin which can also
provide crude product in high purity.