Part No: AN800Issued year: 2003File size: 0.05mbFile type: pdf
The automated Advantage Series™ 3400 ChemCorder™ workflow replicator was used to record all the manual operations a chemist performs during a highly exothermic chemical reaction (Figure 1).Before the experiment was initiated, the ChemCorder replicator was put in record mode.All steps of the experiment were accurately recorded, including any comments and observations. Once completed the ChemCorder replicator produced a report and recipe file of the chemistry. This recipe file was used to accurately and automatically repeat the reaction. Alternatively, the file could have been modified to produce a new recipe based on the recorded chemistry
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates,
1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.
Part No: Issued year: 2005File size: 0.43mbFile type: pdf
PS-DMAP is a polymer-bound equivalent of dimethylaminopyridine (DMAP) which may be used as a catalyst for acylation and related reactions. PS-DMAP has a loading of 1.4 mmol/g based on nitrogen analysis. Typical catalysis conditions require 10 - 20 mol% relative to the nucleophile. Catalytic PS-DMAP accelerates the acylation of sluggish nucleophiles, e.g. tertiary alcohols.
Part No: Issued year: 2007File size: 0.09mbFile type: pdf
PS-TBD (Figure 1) is a polystyrene bound guanidine base, which is stronger than corresponding resin bound DIEA (DIPEA) and bound N-Methyl-morpholine resins. It has a wide variety of uses in synthesis, from direct alkylation through to sequestering of acidic components of reaction.
Part No: P005Issued year: File size: 1.34mbFile type: pdf
An oxidation method using a novel solid phase oxidant, based on a covalently polystyrene bound, sulfonic ester linked 2,2,6,6-tetramethylpiperidine-1-oxyl species is presented, PS-TEMPO (Fig.1a&1b).1,2 The reagent showed fast room temperature kinetics compared to similar known systems3 when used to oxidize primary and secondary alcohols to aldehydes and ketones, respectively (Table 1). The products were in general of high purity and the isolated yields were good to excellent. The used reagent could be reactivated and reused for multiple oxidation reactions. The colour change observed as the oxidation state of the reagent changed, gave an indication of the extent to which the reagent had been used.
Part No: TN521Issued year: 2003File size: 0.05mbFile type: pdf
PS-Thiophenol is based on an aminomethyl resin with a tethered thiophenol functionality. The resin was
designed for the scavenging of alkylating agents, e.g. alkyl halides. PS-Thiophenol was tested and found
effective in scavenging alkylating agents ranging from octyl bromide to benzyl bromide. The scavenging
effectiveness of PS-Thiophenol was found to be greater than a polymer-bound benzyl thiol towards octyl
bromide, indicative of the higher nucleophilicity of the thiophenolate.
Part No: TN513Issued year: 2003File size: 0.03mbFile type: pdf
PS-Trisamine is an amine functional resin for the removal of excess electrophilic reagents during the quenching and purification of reaction mixtures.1 PS-Trisamine resin has a scavenging capacity of 3.0–4.0 mmol/g based on reaction with an excess of benzoyl chloride. Scavenging of common electrophiles typically requires 3–6 equiv of PS-Trisamine and occurs within 0.5–3 h at room temperature. The scope of PS-Trisamine as a scavenger for electrophilic reagents was investigated using 4-chlorobenzoyl chloride, 2-phenylbutyryl chloride and 2,6-dimethoxybenzoyl chloride as a series of acid chlorides with decreasing
reactivity.Acid chlorides were completely scavenged in 0.5 h using 3.5 equiv PS-Trisamine (Table 1). The presence of a tertiary base, e.g. PS-DIEA or PS-NMM, reduces the amount of PS-Trisamine required in the reaction by removing the hydrogen chloride formed. In addition to acid chlorides, benzenesulfonyl chloride and 4-methoxyphenyl isocyanate were effectively scavenged in 0.5 h.
Part No: TN123.06Issued year: 2006File size: 0.07mbFile type: pdf
This Chemistry Data Sheet details the use of ISOLUTE PE-AX, a strong anion exchange sorbent, for the
purification of acidic products from organic synthesis mixtures containing non-acidic reagents or by-products.
Part No: Issued year: 2013File size: 0.67mbFile type: pdf
We have successfully developed a rapid and efficient microwave-assisted protocol for the preparation of Neu5Ac congeners that are important intermediates for clinically-relevant derivatives of Neu5Ac. The syntheses were performed using readily available reagents, improved on established techniques, and used more benign reagent for peracetylation. Moreover, the protocol that we developed is 1 to 2 orders of magnitude faster than conventional methods.
Initiator+ SPWave synthesis system
Dhananjaya Sahoo, I. Abrrey Monreal, and Jonel P. Saludes
Part No: Issued year: File size: 0.18mbFile type: pdf
For high efficiencyin parallel synthesisand method developement short reaction timesare essential. We have examinedthe possibilityof maintaining reported selectivitiesin rapid, high temperaturepalladium-catalyzed asymmetric allylic alkylations. Reactions were promotedby useof singlemode microwave heating(vide infra),which oppositeto conventional oilbath heating(wallheat transfer) rapidlyand almost homogeneouslyheat largeparts of the sample(internalheat transfer).
Part No: Issued year: 2011File size: 0.18mbFile type: pdf
User report: RapidTrace. The Laboratory of Racing Chemistry (LRC) analyzes samples from racehorses for doping drug. In order to screen approximately 1000 samples per week, LRC use an array of Biotage RapidTrace automated SPE systems. In an interview, Mr. Kenji Kinoshita and Mr. Kensaku Shirai talked about their experiences with the RapidTrace system in processing the heavy workload.
Part No: AN704Issued year: 2011File size: 0.04mbFile type: pdf
This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system.
EPH, Massachusetts Department of Environmental Protection,
The RapidTrace+ is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated
pharmaceutical, clinical and forensic laboratories. The RapidTrace+ eliminates SPE bottlenecks so you can realize the
full benefits of today’s powerful analytical instruments.
Part No: AN062Issued year: 2012File size: 1.44mbFile type: pdf
Pure fractions are in high demand – impurities mean more work after purification. With new technology, fraction purity can be digitally analyzed directly during chromatography to reveal any problems on the fly. In this application we will show how the Isolera Spektra is used to determine fraction purity, eliminating the need for other post-purification analysis.
Part No: TV-SS-09Issued year: 2010File size: 0.13mbFile type: pdf
Our TurboVap II customers have been successful obtaining excellent recoveries for all semivolatile organic compounds (BNA, PAH, PCB, Pesticides, TPH/DRO, Herbicides). There are many factors that can impact the recoveries, such as client sample matrix, temperature, humidity, and extraction solvents. After gathering information from our customers, we have developed the guidelines below that will help your laboratory determine its optimum conditions for the TurboVap II.
Part No: Issued year: 2006File size: 0.68mbFile type: pdf
Synthetic oligosaccharides and glycoconjugates provide materials for correlating structure with function. Synthetic mimics of the complex assemblies found on cell surfaces can modulate cellular interactions and are under development as therapeutic agents.
Part No: P056Issued year: 2013File size: 0.42mbFile type: pdf
We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved.
EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.
Part No: P014Issued year: 2006File size: 0.13mbFile type: pdf
One very common transformation in organic synthesis is the reduction of aldehydes or ketones to the corresponding primary or secondary alcohols (Scheme 1) using sodium borohydride as hydride donator. This reaction generally generates the product in very high yield. Among the draw-backs are long reaction times, that a large excess of reagent is needed and that the reaction mixture must be extracted in order to separate the inorganic salts from the product.
Part No: AN074Issued year: 2013File size: 0.52mbFile type: pdf
In this example, Isolera™ Dalton was used to fractionate completely non-UV absorbing starting materials and products from a classic reductive amination reaction. A resin bound cyanborohydride reagent was used to greatly facilitate the work flow by eliminating the need for traditional work-up, and the mixture applied to flash chromatography purification methods.
Part No: PPS342.v2Issued year: 2014File size: 0.3mbFile type: pdf
MIP Technologies AB has developed
a new resin for silver ion chromatography, RENSA® 101.
The surface of the material contains hydrophobic moieties along
with silver ions bound to a sulfonic acid functionality.