Part No: P137v2Issued year: 2016File size: 0.39mbFile type: pdf
This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine).
A number of steps are optimized to improve sensitivity of the
analytes. This includes reducing the buffer strength in the mobile
phase, reducing the buffer strength of washes, careful pH control
throughout the extraction, avoiding an evaporation step and
incorporating IPA in the reconstitution solvent.
Part No: Issued year: 2012File size: 0.11mbFile type: pdf
The following organization’s Quality Management System has been assessed and registered by Intertek Certification AB as conforming to the requirements of: SS-EN ISO 9001:2008
The Quality Management System is applicable to: Development and production of specialty polymers (MIPs and NIPs) for industrial separations and laboratory trace analysis.
Part No: CM-INT-0810Issued year: 2010File size: 0.25mbFile type: pdf
ChemMatrix is a patented, 100% PEG (polyethylene glycol)
based resin developed by PCAS BioMatrix that offers substantial
advantages over traditional PEG based polystyrene resins for
solid phase peptide synthesis.
Part No: RT-CM-0111Issued year: 2010File size: 0.09mbFile type: pdf
In order to keep your RapidTrace® workstation clean, in good working order and free from contamination it is of course essential to
routinely clean and purge the fluid path. Whichever matrix and reagents are running through the system, traces of these liquids will
be left after use; proteins from plasma, precipitates from buffers or even particulates from oral fluid. Over time if the following
cleaning schedule is not observed these contaminants could cause blockages leading to malfunctions and/or cross contamination.
Part No: PPS451Issued year: 2017File size: 2.99mbFile type: pdf
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Part No: P136Issued year: 2015File size: 0.6mbFile type: pdf
This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared.
MSACL EU 2015
Part No: PPS362Issued year: 2014File size: 1.23mbFile type: pdf
This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled
stripped plasma using a supported liquid extraction procedure.
Part No: PPS366Issued year: 2014File size: 1.76mbFile type: pdf
Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: PPS361Issued year: 2014File size: 1.28mbFile type: pdf
This document compares automated sample preparation using the Biotage®
Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: P142Issued year: 2016File size: 0.57mbFile type: pdf
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Part No: AN879Issued year: 2017File size: 0.89mbFile type: pdf
There are a wide range of volatile and semi-volatile contaminants finding their way into both terrestrial bodies and water sources worldwide. In the United States (US), the contaminants are analyzed
according to stipulated US-EPA methods. In the European Union (EU), a large number of these same compounds are tested according to the European Water Framework Directive. Though these analytes are approached differently from a regulatory perspective, it is clear that background monitoring occurs on a global basis. Initial extraction of these analytes depends on the matrix being analyzed and is often a multifaceted process, but ultimately analysts are presented with some form of extraction/organic solvent they must concentrate to achieve instrumental limits of quantification. Presented within this technical note are the results of such an evaporative process using the new Biotage TurboVap® II.
Part No: PPS430Issued year: 2016File size: 0.97mbFile type: pdf
At the Forensic Medicine Lab at Toho University, researchers use ISOLUTE® SLE+ columns from Biotage. When dealing with samples that easily form emulsions like urine or blood, it allows researchers to use the established liquid-liquid extraction technique, saving significant amount of time on analysis. We spoke with the Head of the Forensic Medicine Lab Professor Masaru Terada.