Part No: TN139Issued year: 2011File size: 0.17mbFile type: pdf
This Chemistry Data Sheet provides guidelines for the extraction of basic drugs from biological fluid samples using resin-based mixed-mode SPE. The generic method is described on page 1, with processing and method optimization guidelines on page 2. An example application showing the extraction of basic drugs from plasma illustrates the versatility of EVOLUTE® CX when extracting a wide range of analytes (Procainamide, Salbutamol, Atenolol, Ranitidine, Metoprolol, Brompheniramine).
EVOLUTE, OASIS, CATION EXCHANGE, SPE, SAMPLE PREP, SOLID PHASE EXTRACTION, POLYMER, BASIC
Part No: TN-31.0712Issued year: 2012File size: 0.93mbFile type: pdf
EXPRESS represents a leap forward in high
throughput solid phase extraction. The EVOLUTE
EXPRESS range of 96-well SPE plates combines
powerful EVOLUTE sorbent chemistry with innovative
features that enhance productivity by optimizing
or even eliminating the need for some traditional
steps in the SPE procedure. By truly eliminating the
need for conditioning and equilibration, samples
can be prepared by loading the plate, washing away
interferences and eluting with compatible solvents
Part No: TN146Issued year: 2011File size: 0.23mbFile type: pdf
This technical note provides guidelines for the extraction of strongly acidic compounds from aqueous samples using EVOLUTE WAX. The procedure is described on pages 1, with processing and optimization guidelines on page 2. An example application showing the extraction of pesticide metabolites from urine illustrates the use of EVOLUTE WAX (dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA)) for clean-up of biological fluid samples.
POLYMER, ACIDIC, WEAK ANION EXCHANGER, EVOLUTE, SPE, SAMPLE PREP, SAMPLE PREPARATION, OASIS
Part No: TN143Issued year: 2011File size: 0.12mbFile type: pdf
This technical note provides guidelines for the extraction of quaternary amines and strongly basic analytes from aqueous samples using EVOLUTE WCX (Method A). A method optimized for extraction of a wide range of basic compounds, allowing elution under mildly acidic conditions (Method B) is also detailed. This approach is recommended for the extraction of basic compounds when basic elution conditions are unsuitable, or acidic elution conditions are preferred due to LC-MS/MS compatibility. The procedures are described on page 1–2, with processing and optimisation guidelines on page 3. An example application showing the extraction of quaternary amines from urine and plasma illustrates the use of EVOLUTE WCX (Chlormequat, Neostigmine, Bretylium, Difenzoquat) for clean-up of biological fluid samples.
BASIC, BIOTAGE, EVOLUTE, OASIS, SPE, POLYMER, SOLID PHASE EXTRACTION
Part No: TN-0026.0311Issued year: 2011File size: 0.46mbFile type: pdf
EVOLUTE® WAX mixed mode polymer-based SPE sorbent
extracts a range of acidic analytes from biological fluids and
other aqueous matrices using a generic procedure which
minimizes method development time. EVOLUTE WAX removes
matrix components such as proteins, salts, non-ionisable
interferences and phospholipids, delivering cleaner extracts
with reproducible recoveries for accurate quantitation.
Part No: TN-0024.0210Issued year: 2010File size: 0.35mbFile type: pdf
EVOLUTE® WCX is a weak cation exchange mixed-mode
resin-based SPE sorbent that extracts a range of strongly
basic analytes and quaternary amines from biological fluids
and other aqueous matrices using a generic procedure
which minimizes method development time. EVOLUTE WCX
removes matrix components such as proteins, salts, nonionizable
interferences and phospholipids, delivering
cleaner extracts with reproducible recoveries for accurate
Part No: TN131Issued year: 2004File size: 0.26mbFile type: pdf
EVOLUTE™ Sample Preparation Products are a new generation of advanced polymeric solid
phase extraction sorbents for the high throughput extraction of drugs from biological fluids.
Designed to maximize analyte recovery,while reducing the concentration of matrix components
in the final extract, EVOLUTE products provide improved extract cleanliness that minimizes ion
suppression effects during LC-MS/MS analysis.
Part No: TN-0012.0610Issued year: 2010File size: 0.38mbFile type: pdf
Through innovative patented technology molecularly
imprinted polymers (MIPs) can afford improved clean-up in
your processes. Unwanted contaminants or high value
desirables can be efficiently extracted from processes
streams, resulting in more efficient production and cleaner
Part No: AN717Issued year: 2010File size: 0.48mbFile type: pdf
Pharmaceutical companies and those who supply them with intermediates, constantly strive to produce API’s and
intermediates of the highest purity. At the same time, many established production facilities face strong competitive
pressures to produce high quality product at lower costs. Drug substance impurities are wide-ranging as described in the
International Conference on Harmonization (ICH) “Impurities in new Drug Substances”1. Of particular concern in recent
years are the ‘genotoxic compounds.
Part No: P128v2Issued year: 2016File size: 0.4mbFile type: pdf
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
Part No: AN715Issued year: 2011File size: 0.13mbFile type: pdf
This method demonstrates the extraction of a PAH standard test mix comprising of fluoranthene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(123-cd)pyrene, dibenzo(ah)anthracene, and benzo(ghi)perylene.
MIP, AFFINILUTE, SupelMIP, BIOTAGE, PAH, Polyaromatic Hydrocarbons
Part No: AN833Issued year: 2015File size: 1.33mbFile type: pdf
This application note describes a polymer based-based weak exchange mixed-mode SPE protocol for the extraction of the aminoglycoside antibiotics gentamicin, streptomycin, spectinomycin and neomycin from chicken meat prior to LC-MS/MS analysis. Linearity and LOQ meet USDA-FSIS guidelines.
Part No: P120Issued year: 2015File size: 0.63mbFile type: pdf
This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego
Part No: AN778Issued year: 2013File size: 0.88mbFile type: pdf
This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes.
Cortisol, Saliva, SLE, Supported Liquid Extraction, ISOLUTE, Clinical, Steroid Hormones, Stress
Part No: AN777Issued year: 2013File size: 0.86mbFile type: pdf
Cortisol is a steroid hormone that in urine can be used to diagnose hyper or hypo cortisol diseases such as Cushing’s Syndrome. This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of urinary cortisol levels. Analyte recoveries achieved using this method ranged from 99-101% with RSDs below 5% for all analytes.
Cortisol, Steroid hormone, Cushing's Syndrome, Cushing's Disease, Clinical, SLE, Supported Liquid Extraction, Stress,
Part No: AN841Issued year: 2015File size: 0.82mbFile type: pdf
This application note describes the extraction of 11-nor-9-carboxy-THC from a urine matrix, prior to GC/MS analysis.
Carboxy-THC is the primary metabolite of THC, a key indicator of illicit marijuana usage. In urine, ~80% of the carboxy-THC metabolite is present in the form of its glucuronide metabolite. Therefore, to effectively quantify the THC-COOH, urine is hydrolyzed before extraction. This application note describes optimized extraction of urine samples prepared by either enzymatic or base hydrolysis.