Part No: AN795Issued year: 2013File size: 1.09mbFile type: pdf
This application note describes a method of extraction for metanephrine and normetanephrine from synthetic
urine using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction technology has been developed to reduce
the time associated with sample preparation methods by
eliminating steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
Part No: AN080Issued year: 2013File size: 0.26mbFile type: pdf
This application note describes a method for sample preparation
of different matrixes prior to amino acid analysis according to IS
ISO 13903:2005 or the similar AOAC Official Method 994.12 using
Biotage® Initiator+ to speed up the process.
Part No: PPS436Issued year: 2016File size: 0.4mbFile type: pdf
Sanford Burnham Prebys Case Study.
Fewer transfers and the ability to evaporate difficult solvents right in
the scintillation vial has made Biotage® V-10 the go-to evaporator at
Sanford Burnham Prebys (SBP), a top medical research institute.
Part No: TN-0012.0806Issued year: 2008File size: 0.09mbFile type: pdf
Biotage FLASH 400™ Production-Scale System The FLASH 400 is a complete skid-mounted system designed for large-scale flash chromatography and adsorption purification. The FLASH 400 uses prepacked cartridges and radial compression for performance and reliability. Built with industrial-grade components that are appropriate for operations under FDA regulations and cGMP standards, the FLASH 400 is rapidly becoming the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.
Part No: AN059Issued year: 2012File size: 0.63mbFile type: pdf
Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.
Part No: AN060Issued year: 2012File size: 1.02mbFile type: pdf
Isolera Spektra provides definitive fraction purity assessment
in both 2-D and 3-D prior to any post flash analysis. With this
information chemists quickly know if a fraction contains a pure
product suitable for further mass and structure confirmation
or whether the impure fractions must be re-purified. This
knowledge improves synthesis throughput and avoids any
embarrassment of submitting impure fractions for analytical
Part No: AN092.v1Issued year: 2014File size: 1.12mbFile type: pdf
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.
Part No: AN095Issued year: 2014File size: 0.65mbFile type: pdf
Elution of polar molecules in flash chromatography sometimes requires solvent additives, making purification more cumbersome. The Isolera™ family of flash purification instruments and Biotage® SNAP KP-NH cartridges significantly reduce the extra work involved.
Part No: TN-0006.0806Issued year: 2006File size: 0.86mbFile type: pdf
Purify up to 400 grams of compound at 1 L/min with the Biotage Flash 150™ system, up to 2x faster than traditional glass columns. This robust stainless steel system safely operates at 100 psi enabling high flow rates and the use of higher viscosity solvents. A variety of cartridge media provides
chemists with selectivity choices for optimal purification conditions. Simple and reliable, this system contains everything needed to begin your separations.
Part No: TN407.1107Issued year: 2007File size: 0.07mbFile type: pdf
FlashVac-10 and -20 Sample Processing Manifolds are used
to process standard Luer tipped ISOLUTE work-up columns.
Constructed from glass or high density polyethylene, the
FlashVac manifolds are compatible with commonly used
reaction and work-up solvents.
Part No: AN086.v2Issued year: 2014File size: 1.02mbFile type: pdf
A novel resin from MIP Technologies, RENSA® PY, is able to fractionate colored compounds present in beetroot juice using mild and purely aqueous elution conditions without the use of salts or organic solvents.
Part No: Issued year: 2015File size: 1.88mbFile type: pdf
User Report: Isolera™ Dalton, Okayama University. The laboratory of Prof. Hiroyuki Miyachi at Okayama University installed Biotage’s Isolera Dalton, an automated mass-directed purification system for flash chromatography, allowing the fractionation and collection of optical isomers. “I felt like the world’s first mass-detection automated purification system had finally arrived.”
Part No: AN792Issued year: 2013File size: 2.3mbFile type: pdf
This application note describes fully automated liquid handling of the extraction of a range of drugs from urine, which are typically screened for forensic toxicology panels, using ISOLUTE® SLE+ 96-well supported liquid extraction plates.
drugs of abuse, TECAN, automated, ISOLUTE SLE+
Part No: TN113Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.