Part No: AN802Issued year: 2013File size: 1.19mbFile type: pdf
This application note describes effective and efficient protocols optimized for sample volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs of <10% for all analytes.
Part No: AN856Issued year: 2015File size: 0.7mbFile type: pdf
This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Part No: AN602Issued year: 2011File size: 0.23mbFile type: pdf
This Application Note highlights the use of ISOLUTE® SLE+ supported-liquid extraction plates for the extraction of corticosteroids from human plasma, specifically Triamcinolone, Prednisolone, Hydrocortisone, Prednisone, Betamethasone, Cortisone, Dexamethasone, Flumethasone, Corticosterone, Beclomethasone, Triamcinolone, Fluocinolone, Budesonide 1, Budesonide 2 and 5-Pregnen-3β−ol-20-one.
Part No: P019Issued year: 2007File size: 0.74mbFile type: pdf
Naturally occurring corticosteroids are steroid hormones produced in the adrenal cortex and perform many functions within the body. Due to their widespread action
many synthetic corticosteroids based on the hydrocortisone (cortisol) structure have been produced. Some of their uses involve treatment of various forms of arthritis, dermatitis, hepatitis and ulcerative colitis. They can be administered orally, injected into veins or muscle, nasal sprays and drops or used as topical creams. Reliable
screening methods for these drugs are important as they are classed as ‘banned substances’ by many anti-doping agencies and regulated for use in meat producing animals.
Part No: AN808Issued year: 2013File size: 1.2mbFile type: pdf
This application note describes the extraction of a range of recreational ecstasy-like drugs, designer stimulants and the hallucinogen 2C-B from urine prior to GC/MS analysis. The method described in this application note may also be suitable for extraction of other related analogs and derivatives of these drug compounds, for example the substituted cathinone class (bathsalts). This application note is optimized for extraction of 1 mL sample volumes.
Part No: AN887Issued year: 2017File size: 1.45mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis.
The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.
Part No: AN700Issued year: 2011File size: 0.07mbFile type: pdf
This procedure is recommended for the extraction of a variety of diuretics from urine using non-polar polymeric SPE. The analyte suite includes thiazides, carbonic anhydrase inhibitors, loop and potassium sparring diuretics, with wide ranging pKa and logP values.
Biotage, polymer, resin,
Part No: AN844Issued year: 2015File size: 0.75mbFile type: pdf
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
Part No: AN860Issued year: 2016File size: 0.89mbFile type: pdf
This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used.
High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
Part No: AN748Issued year: 2011File size: 0.23mbFile type: pdf
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Part No: AN714Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed and optimized for the extraction of fluoroquinolones (FQL) from a variety of sample matrixes including bovine kidney, honey, and milk. Example FQLs include sarafloxacin, norfloxacin, enrofloxacin, ciprofloxacin, lomefloxacin, and ofloxacin.
Part No: AN713Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of the tobacco specific nitrosamine, NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) from aqueous sample matrices such as biological fluids (e.g., urine). The method is highly reproducible and offers NNAL recoveries in the range of 90% and a limit of detection below 5 ppt.
AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP
Part No: AN828Issued year: 2014File size: 1.48mbFile type: pdf
This application note describes the extraction of the 'date rape' drug GHB (gamma-hydroxybutyric acid) from urine using supported liquid extraction and subsequent analysis by GC/MS.
Both 96-well plate and column formats are included, and demonstrate good analyte recovery and reproducibility, at levels appropriate for determining illicit use of GHB.
Part No: AN864Issued year: 2016File size: 2.43mbFile type: pdf
This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
A protocol has been developed that allows the simultaneous
extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC.
In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Part No: AN788Issued year: 2013File size: 1.57mbFile type: pdf
This application note describes the extraction of a range of illicit drugs, which are typically screened in forensic toxicology panels, from oral fluid using ISOLUTE® SLE+ supported liquid extraction columns.
oral fluid, Quantisal, Immunalysis, Intercept, Orasure, supported liquid extraction
Part No: AN762Issued year: 2012File size: 0.08mbFile type: pdf
This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%.
Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,
Part No: IST1041AIssued year: 2011File size: 0.14mbFile type: pdf
This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism.
LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen
Part No: AN765Issued year: 2012File size: 0.22mbFile type: pdf
Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green.
Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,