Part No: AN808Issued year: 2013File size: 1.2mbFile type: pdf
This application note describes the extraction of a range of recreational ecstasy-like drugs, designer stimulants and the hallucinogen 2C-B from urine prior to GC/MS analysis. The method described in this application note may also be suitable for extraction of other related analogs and derivatives of these drug compounds, for example the substituted cathinone class (bathsalts). This application note is optimized for extraction of 1 mL sample volumes.
Part No: AN887Issued year: 2017File size: 1.45mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis.
The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.
Part No: AN700Issued year: 2011File size: 0.07mbFile type: pdf
This procedure is recommended for the extraction of a variety of diuretics from urine using non-polar polymeric SPE. The analyte suite includes thiazides, carbonic anhydrase inhibitors, loop and potassium sparring diuretics, with wide ranging pKa and logP values.
Biotage, polymer, resin,
Part No: AN844Issued year: 2015File size: 0.75mbFile type: pdf
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
Part No: AN860Issued year: 2016File size: 0.89mbFile type: pdf
This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used.
High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
Part No: AN748Issued year: 2011File size: 0.23mbFile type: pdf
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Part No: AN714Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed and optimized for the extraction of fluoroquinolones (FQL) from a variety of sample matrixes including bovine kidney, honey, and milk. Example FQLs include sarafloxacin, norfloxacin, enrofloxacin, ciprofloxacin, lomefloxacin, and ofloxacin.
Part No: AN713Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of the tobacco specific nitrosamine, NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) from aqueous sample matrices such as biological fluids (e.g., urine). The method is highly reproducible and offers NNAL recoveries in the range of 90% and a limit of detection below 5 ppt.
AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP
Part No: AN828Issued year: 2014File size: 1.48mbFile type: pdf
This application note describes the extraction of the 'date rape' drug GHB (gamma-hydroxybutyric acid) from urine using supported liquid extraction and subsequent analysis by GC/MS.
Both 96-well plate and column formats are included, and demonstrate good analyte recovery and reproducibility, at levels appropriate for determining illicit use of GHB.
Part No: AN864Issued year: 2016File size: 2.43mbFile type: pdf
This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
A protocol has been developed that allows the simultaneous
extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC.
In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Part No: AN788Issued year: 2013File size: 1.57mbFile type: pdf
This application note describes the extraction of a range of illicit drugs, which are typically screened in forensic toxicology panels, from oral fluid using ISOLUTE® SLE+ supported liquid extraction columns.
oral fluid, Quantisal, Immunalysis, Intercept, Orasure, supported liquid extraction
Part No: AN762Issued year: 2012File size: 0.08mbFile type: pdf
This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%.
Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,
Part No: IST1041AIssued year: 2011File size: 0.14mbFile type: pdf
This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism.
LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen
Part No: AN765Issued year: 2012File size: 0.22mbFile type: pdf
Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green.
Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,
Part No: AN733Issued year: 2011File size: 0.21mbFile type: pdf
This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible.
food scare, Melamine, China, kidney failure, renal failure, protein, Biotage, EVOLUTE,
Part No: AN732Issued year: 2011File size: 0.21mbFile type: pdf
This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Melamine's low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products.
Biotage, cation exchange, oasis, food scare, china,
Part No: AN749Issued year: 2011File size: 0.28mbFile type: pdf
Melamine is traditionally used in making plastics, however, it’s low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows recoveries of 88-90% with RSDs <10%.
Melamine, food scare, china, EVOLUTE, cation exchange, RapidTrace, Automation, SPE, sample prep,
Part No: AN743Issued year: 2011File size: 0.16mbFile type: pdf
This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%.
SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA
Part No: P034Issued year: 2012File size: 0.48mbFile type: pdf
The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly
used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems
Part No: AN847.V.1Issued year: 2016File size: 2.48mbFile type: pdf
This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates.
Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.