Part No: AN750Issued year: 2012File size: 1.14mbFile type: pdf
This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant.
Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,
Part No: AN701Issued year: 2007File size: 0.07mbFile type: pdf
This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency.
Pharmaceuticals, waste water, antibiotics, EVOLUTE,
Part No: IST1019AIssued year: 2011File size: 0.14mbFile type: pdf
This method was developed for the extraction of phencyclidine from meconium for drugs ofabuse applications. The analyte has an ionisable amine group, which is exploited through cation exchange interactions. The pKa is approximately 8, so the compound is fully ionized at pH values of less than 6. The analyte also has significant non-polar characteristics, and both hydrophobic and ion exchange retention mechanisms are used to give a clean extract.
ISOLUTE, PCP, DOA, Varian Bond Elut Certify, UCT CleanScreen DAU
Part No: IST1039AIssued year: 2011File size: 0.14mbFile type: pdf
This application was developed for the extraction of phencyclidine and other basic drugs from urine for drugs of abuse applications. Typical recovery for PCP is > 85%.
PCP, DOA, Biotage, ISOLUTE, UCT CleanScreen DAU, Varian Bond Elut Certify
Part No: AN876Issued year: 2017File size: 2.77mbFile type: pdf
Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.
The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Part No: AN798Issued year: 2013File size: 1.33mbFile type: pdf
This application note describes a novel sample cleanup strategy to address the complex matrix effects associated
with commercial milk product variables while maintaining adequate analyte recovery and repeatability.
Part No: IST1025A.V.1Issued year: 2016File size: 0.66mbFile type: pdf
This application note describes the extraction of polyaromatic hydrocarbons (PAHs) from natural waters with a high concentration
of humic acids. Layered SPE columns are used to eliminate humic acids from the final extract.
Part No: AN883Issued year: 2017File size: 1.33mbFile type: pdf
The method described in this application achieves high reproducible recoveries for a number of common PFCs in water using an optimized weak cation exchange SPE procedure with EVOLUTE® EXPRESS WAX SPE columns.
Part No: P133Issued year: 2015File size: 0.51mbFile type: pdf
This poster demonstrates a simple supported liquid extraction procedure for the extraction of propofol from whole blood suitable for GC-MS analysis.
In addition, phospholipid profiles in the extracts were investigated using LC-MS/MS, and showed no breakthrough of these blood components, suggesting the procedure is appropriate for use with LC-MS/MS based analytical procedures.
Part No: AN753Issued year: 2011File size: 0.33mbFile type: pdf
Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from human serum prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from serum allowing for mass spectral detection of the target analytes at a concentration of 100 ng/mL or less.
Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Serum, Clinical, LCMS, Sample Prep
Part No: AN754Issued year: 2011File size: 0.3mbFile type: pdf
Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from whole blood prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from whole blood allowing for mass spectral detection of the target analytes at a concentration of 200 ng/mL or less.
Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Whole Blood, Clinical, LCMS, Sample Prep
Part No: AN774Issued year: 2012File size: 0.19mbFile type: pdf
This application note describes the extraction and quantitation of Cannabimimetic Naphthoylindoles (Synthetic Cannabinoids) and their metabolites (JWH Series) from various matrices using Supported Liquid Extraction (SLE). Synthetic Cannabinoids or SPICE as they are commonly known have become an increasing problem as one of the newest forms of illicit drugs being consumed today. These compounds bind to the cannabinoid receptors in mammals triggering similar euphoric symptoms as Tetrahydrocannabinoids (THC). Currently robust and fast analytical methods of analysis are required to aid in the screening and detection of this growing class of compounds. The recoveries obtained for the synthetic cannabinoids parent and metabolites ranged from 70-98 %.
Supported Liquid Extraction, SLE, SLE+, SPICE, Synthetic Cannabinoids, JWH, metabolites,
Part No: AN791Issued year: 2013File size: 3.25mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.
SPICE, oral fluid, Intercept Orasure, LC-MSMS, SLE
Part No: AN779Issued year: 2013File size: 1.2mbFile type: pdf
This application note describes the extraction of a range of synthetic cannabinoids and metabolites from oral fluid collected using the Quantisal™ Oral Fluid Collection Device prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for loading volumes of either 400 μL or 1 mL of matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs <10% for all analytes.
Part No: AN773Issued year: 2013File size: 1.25mbFile type: pdf
This application note describes the extraction of a range of synthetic cannabinoids and metabolites from whole blood prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for extraction of 800 μL of pre-treated matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs lower than 10% for all analytes.
Part No: P075Issued year: 2014File size: 0.66mbFile type: pdf
Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and
quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we
demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices.