Part No: P160Issued year: 2017File size: 0.24mbFile type: pdf
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Part No: PPS375.v1Issued year: 2015File size: 0.3mbFile type: pdf
User report: Flash instruments. Chugai Pharmaceutical uses Biotage flash chromatography products for drug discovery research. When deciding to convert from manual open-column procedures to automated systems, they chose successive generations of Biotage products, ranging from the Flash+® packed column to the Biotage® Horizon, SP1, Isolera™ Spektra, and Isolera™ Dalton automated flash chromatography systems.
Part No: AN041Issued year: 2001File size: 0.16mbFile type: pdf
Normal-phase flash purification is commonly used by organic chemists in pharmaceutical drug discovery and
process development labs. However, for many synthesized products (e.g. peptides, nucleotides and basic
drug candidates) purification on standard flash silica is not an option due to irreversible adsorption, chemical
interaction and/or solubility issues. Reversed-phase flash purification is an excellent solution for these applications. Yet, this technique has been used sparingly because of perceived lower loading capacity, higher
operating pressures and a scarcity of publications addressing reversed-phase flash chromatography.
Part No: AN039Issued year: 2002File size: 0.64mbFile type: pdf
For pilot and production scale purification drugs, the use of a large particle-size media is common. Improving the purification throughput is limited by the media’s large particle size. Such a case was recently encountered when a pharmaceutical company attempted to improve the efficiency of a large-scale purification. In their current process, the use of a 350-600 µm polystyrene-type resin resulted in a lengthy purification cycle and low separation efficiency. However, the labile properties of the targeted component required a shorter purification cycle and high purification.
Part No: TN128Issued year: 2006File size: 0.09mbFile type: pdf
This technical note describes the use of derivatization techniques to separate aliphatic
secondary amines from aliphatic tertiary amines in a mixture using the strong cation
exchange sorbent, ISOLUTE® SCX-2.
Part No: PPS385Issued year: 2015File size: 4.87mbFile type: pdf
Our carefully selected portfolio of industrial scale products have a proven track record of successful applications and use in scale-up projects. We can support the discovery, development and manufacturing of customer pharmaceutical and biotechnology products, from pre-clinical, phase I, II and III to small scale commercial operations.
Part No: TN-0028.1222Issued year: 2012File size: 1.6mbFile type: pdf
The Initiator+ is a flexible system that utilizes all Biotage vials, from 0.2 to 20 mL,
in any order or combination, at any time without system modifications, delivering greater flexibility and direct scale-up from milligrams to grams.
Part No: TN-0029.0611Issued year: 2011File size: 1.07mbFile type: pdf
In peptide synthesis mode, this is the perfect tool for chemists synthesizing peptides and peptidomimetics, including difficult modifications and labeling of sequences, by using single or multi-step procedures. The intuitive Initiator 4.0 software controls the instrument functions and by using the pre-defined methods, synthesis can be started immediately.
Part No: AN090Issued year: 2014File size: 1.95mbFile type: pdf
In mass detection, the presence of ions can suppress peaks
from the analytes of interest. This study concludes that
Isolera™ Dalton performs successful separations even with
ions present in the sample.
Part No: AN088.v1Issued year: 2014File size: 1.59mbFile type: pdf
Fragrance compounds, being invisible to UV, can be
elusive and difficult to isolate. In this application, a four
component mixture of fragrance compounds was purified
using Accelerated Chromatographic Isolation™ (ACI) and
identified by mass detection.
Part No: AN600Issued year: 2005File size: 0.08mbFile type: pdf
This Application Note reviews the use of ISOLUTE PPT+ plates for the isolation of drugs from biological fluids, including the method, recovery data and the impact of the filtrate’s cleanliness on analyte quantitation.