Part No: Issued year: 2013File size: 2.62mbFile type: pdf
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
» Isocratic separations are easiest to develop, but gradient separations are
» Software in the Isolera helps with conversion of an isocratic separation to
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
Part No: TN119Issued year: 2006File size: 0.11mbFile type: pdf
This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3).
Part No: TN108 rev1.3Issued year: 2004File size: 0.13mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE CBA for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchange sorbents (pages 4-6).
Part No: TN105 rev1.3Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes), specifics on the use of ISOLUTE® PSA for the extraction of anionic
analytes from aqueous samples (page 1-2), ordering information (page 3) and a general discussion
on anion exchangers (pages 4-5). For information on extraction of anionic analytes from nonaqueous
matrices, contact Argonaut Technologies.
Part No: P119Issued year: 2015File size: 0.45mbFile type: pdf
This poster describes the supported liquid extraction method development process for the extraction of the NBOMes group of novel psychoactive substances from oral fluid, for analysis using GC-MS. both sample pre-treatment and elution solvent were optimised, and the final method compared to traditional LLE and SPE procedures.
Part No: IST1022Issued year: 2006File size: 0.03mbFile type: pdf
Method Development Recommendations for Aflotoxins from Cereals. This represents recommendations for SPE meth
od development. The proposed steps are
based on experience with similar analytes an
d matrices, but have not been verified in
Part No: P065Issued year: 2014File size: 3.65mbFile type: pdf
This poster demonstrates method development strategies for drugs of abuse screening using supported liquid extraction in a 96-well plate and subsequent method scalability to column format to allow larger matrix volume processing prior to UPLC-MS/MS analysis.
Part No: AN726Issued year: 2011File size: 0.17mbFile type: pdf
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Part No: AN727Issued year: 2011File size: 0.11mbFile type: pdf
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Part No: AN728Issued year: 2011File size: 0.15mbFile type: pdf
Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid.
Cyclosporine, Cyclosporine, Immunosuppressant,
Part No: AN729Issued year: 2011File size: 0.18mbFile type: pdf
This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation.
Opiates, drug testing, DOA, Ion exchange
Part No: AN725Issued year: 2011File size: 0.16mbFile type: pdf
This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.
Cocaine, BZE, Benzoylecgonine, drug testing, cocaine metabolite,
Part No: P092Issued year: 2014File size: 0.36mbFile type: pdf
The fermentation process associated with the manufacture of food grade enzyme powders was recently determined to support the colonization of bacteria. To control this issue, manufactures following different international regulatory procedures have found the addition of familiar antibiotic compounds to manufacturing processes beneficial. This becomes an issue of public health as the concentration of antibiotic residues in food grade materials promotes unknown exposure to the global community. Continued interest in the biomonitoring of these compounds have inspired a number of method development strategies; however, classic methods are labor intensive and require multi-step time consuming efforts. The FDA has recently mandated a method for the determination of chloramphenicol in shellfish. This study evaluates the feasibility of method modifications to incorporate enzyme powder matrices into this method. Linearity was determined over a range of 0.1 – 5 ng/mL (r^2 > 0.990). Relative recovery was determined >80%. The typical repeatability (%RSD) for n=7 replicates <20%. Strategies to control solids prior to SPE load will be presented as they were determined critical in method performance. Method robustness was determined by evaluating multiple lots and multiple variants of enzyme powder origin. The key variable is the nature of the excipients as high level sugars of different sources need to be controlled to minimize the ESI suppression.
Part No: P154Issued year: 2017File size: 0.47mbFile type: pdf
Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize
sensitivity allowing low level analysis of vitamin B7 from human serum.
MSACL 2017, Palm Springs
Part No: Issued year: 2016File size: 2.34mbFile type: pdf
This poster describes a validated LC-MS/MS method for the quantification of nicotine, its metabolites: cotinine, trans-3’-hydroxycotinine, and the tobacco plant alkaloid anabasine in patient urine to determine their clinical utility for the evaluation of active smokers for pre-transplant eligibility.
Disclaimer: Publication of this poster in no way implies or construes endorsement of Biotage products by Emory University School of Medicine.
Part No: P146Issued year: 2016File size: 0.6mbFile type: pdf
This poster compares various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of extraction techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression, phospholipid removal and simplicity of transfer to an automated sample preparation platform.
Part No: P006Issued year: File size: 0.09mbFile type: pdf
The formation of 2,5-diketopiperazines from a water suspension of N-Boc protected dipeptide alkyl esters or dipeptide alkyl esters was induced by microwave assisted heating (Scheme 1). The products, which precipitated, were obtained in good yields. However, epimerisation/diastereomer formation was found to various extents depending on the amino acid sequence and the applied reaction temperature.
Part No: AN111Issued year: 2015File size: 1.19mbFile type: pdf
The cyclic peptide mupain-1 (CPAYSRYLDC) is an inhibitor of the cancer-related urokinase-type plasminogen activator. Here we show the synthesis of a non-natural arginine building block precursor using MAOS.