Part No: Issued year: 2016File size: 2.34mbFile type: pdf
This poster describes a validated LC-MS/MS method for the quantification of nicotine, its metabolites: cotinine, trans-3’-hydroxycotinine, and the tobacco plant alkaloid anabasine in patient urine to determine their clinical utility for the evaluation of active smokers for pre-transplant eligibility.
Disclaimer: Publication of this poster in no way implies or construes endorsement of Biotage products by Emory University School of Medicine.
Part No: P146Issued year: 2016File size: 0.6mbFile type: pdf
This poster compares various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of extraction techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression, phospholipid removal and simplicity of transfer to an automated sample preparation platform.
Part No: P006Issued year: File size: 0.09mbFile type: pdf
The formation of 2,5-diketopiperazines from a water suspension of N-Boc protected dipeptide alkyl esters or dipeptide alkyl esters was induced by microwave assisted heating (Scheme 1). The products, which precipitated, were obtained in good yields. However, epimerisation/diastereomer formation was found to various extents depending on the amino acid sequence and the applied reaction temperature.
Part No: AN111Issued year: 2015File size: 1.19mbFile type: pdf
The cyclic peptide mupain-1 (CPAYSRYLDC) is an inhibitor of the cancer-related urokinase-type plasminogen activator. Here we show the synthesis of a non-natural arginine building block precursor using MAOS.
Part No: AN110Issued year: 2015File size: 0.72mbFile type: pdf
PNA oligomers have interesting properties such as their increased thermal and chemical stability and resistance to enzymatic degradation which drives their use in various molecular biology, molecular diagnostic, microarray, biosensor and antisense applications. In this study we synthesize two PNA oligomers on a small scale using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer.
Part No: Issued year: 2004File size: 0.09mbFile type: pdf
Solid-Assisted Solution Phase Synthesis (SASPS) is a technique where the target molecule is synthesized in solution and solid-supported molecules are used as reagents, catalysts or scavengers. Recently this technique has been getting a lot of attention as an alternative to solid phase organic synthesis (SPOS), since it offers many of the advantages of SPOS in terms of ease of reaction workup and product purification in addition to the advantages associated with traditional solution-phase chemistry (e.g. the ease of monitoring the progress of the reaction by simply applying LC-MS or TLC techniques).
Part No: P043Issued year: File size: 0.31mbFile type: pdf
The application of microwave irradiation in solid
phase peptide synthesis (SPPS), is an important tool
for the synthesis of difficult and long peptide
sequences that are often obtained in poor quality by
comparable conventional methods at room
temperature. Here we present the results obtained
for the synthesis of a number of difficult peptide
sequences using different levels of automation for
microwave assisted SPPS: manual, semi-automated
and fully automated systems. Syro wave, initiator, sp wave
Speeding up reactions has never been easier. Biotage microwave
synthesizers are the first-choice tools for organic chemists who
need to accelerate their production of new compounds.
Initiator, Initiator+, robot, vials, phase separator, work up tools
Part No: SS-INT.R 0611Issued year: 2011File size: 1.28mbFile type: pdf
The 60-position sample bed supports the production of focused libraries, multi-user environments and scale-out. Flexible operation enables the use of both large and small vials in combination at any time and in any order without manual intervention.
Part No: TN-VIAL.0811Issued year: 2011File size: 0.17mbFile type: pdf
Biotage® high precision microwave vials are
engineered for durability and safe reactions.
All vials are designed to withstand pressures
beyond 30 bar, and in a wide range of conditions. Technical Specification for Biotage Microwave Vials.
Part No: Issued year: File size: 0.18mbFile type: pdf
Solid-phase synthesis combined with fast and
precise heating by microwave irradiation has
become a valuable tool for peptide chemists. It
enables easier solid-phase assembly of difficult
peptide sequences and even small proteins. Here
we present, a study of microwave assisted solidphase
peptide synthesis using different resins on
the Syro WaveTM. Furthermore, we use this
methodology to synthesize difficult peptide
sequences including a multiple display
Part No: Issued year: File size: 0.45mbFile type: pdf
This paper evaluates solvent effect upon flash chromatography purification. The impact of sample mass overloading, volume overloading and sample dissolution solvents upon sample resolution are discussed.