Part No: Issued year: 2013File size: 0.67mbFile type: pdf
We have successfully developed a rapid and efficient microwave-assisted protocol for the preparation of Neu5Ac congeners that are important intermediates for clinically-relevant derivatives of Neu5Ac. The syntheses were performed using readily available reagents, improved on established techniques, and used more benign reagent for peracetylation. Moreover, the protocol that we developed is 1 to 2 orders of magnitude faster than conventional methods.
Initiator+ SPWave synthesis system
Dhananjaya Sahoo, I. Abrrey Monreal, and Jonel P. Saludes
Part No: Issued year: File size: 0.18mbFile type: pdf
For high efficiencyin parallel synthesisand method developement short reaction timesare essential. We have examinedthe possibilityof maintaining reported selectivitiesin rapid, high temperaturepalladium-catalyzed asymmetric allylic alkylations. Reactions were promotedby useof singlemode microwave heating(vide infra),which oppositeto conventional oilbath heating(wallheat transfer) rapidlyand almost homogeneouslyheat largeparts of the sample(internalheat transfer).
Part No: Issued year: 2011File size: 0.18mbFile type: pdf
User report: RapidTrace. The Laboratory of Racing Chemistry (LRC) analyzes samples from racehorses for doping drug. In order to screen approximately 1000 samples per week, LRC use an array of Biotage RapidTrace automated SPE systems. In an interview, Mr. Kenji Kinoshita and Mr. Kensaku Shirai talked about their experiences with the RapidTrace system in processing the heavy workload.
Part No: AN704Issued year: 2011File size: 0.04mbFile type: pdf
This Application Note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE EPH column in conjunction with the RapidTrace SPE automation system.
EPH, Massachusetts Department of Environmental Protection,
Part No: RT-BR-01Issued year: 2010File size: 0.84mbFile type: pdf
Advanced bioanalytical techniques like LC/MS/MS and GC/MS are meeting the increasing demand for greater speed and specificity in toxicological screening. But conventional sample preparation methods have not kept pace. In particular, solid phase extraction (SPE), an essential step in the analysis of many biomolecules, has become a through-put limiting step in many laboratories.
The RapidTrace+ is a robust automated platform for quickly developing rugged, reliable SPE methods in regulated
pharmaceutical, clinical and forensic laboratories. The RapidTrace+ eliminates SPE bottlenecks so you can realize the
full benefits of today’s powerful analytical instruments.
Part No: AN062Issued year: 2012File size: 1.44mbFile type: pdf
Pure fractions are in high demand – impurities mean more work after purification. With new technology, fraction purity can be digitally analyzed directly during chromatography to reveal any problems on the fly. In this application we will show how the Isolera Spektra is used to determine fraction purity, eliminating the need for other post-purification analysis.
Part No: Issued year: 2006File size: 0.68mbFile type: pdf
Synthetic oligosaccharides and glycoconjugates provide materials for correlating structure with function. Synthetic mimics of the complex assemblies found on cell surfaces can modulate cellular interactions and are under development as therapeutic agents.
Part No: P056Issued year: 2013File size: 0.42mbFile type: pdf
We detail here the application of new column technology to purify histamine and serotonin (Table 1) in one extraction method with the elimination of the sorbent conditioning and equilibration steps familiar to traditional solid phase extraction (SPE) method protocols prior to measurements by ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-MS/MS). A secondary goal of combining two LC methods into one method was also achieved.
EVOLUTE EXPRESS cation exchange (CX), weak cation exchange (WCX) and acid-base-neutral (ABN) sorbents in a 96 well plate format prior to analysis by UPLC-MS/MS.
Part No: P014Issued year: 2006File size: 0.13mbFile type: pdf
One very common transformation in organic synthesis is the reduction of aldehydes or ketones to the corresponding primary or secondary alcohols (Scheme 1) using sodium borohydride as hydride donator. This reaction generally generates the product in very high yield. Among the draw-backs are long reaction times, that a large excess of reagent is needed and that the reaction mixture must be extracted in order to separate the inorganic salts from the product.
Part No: AN074Issued year: 2013File size: 0.52mbFile type: pdf
In this example, Isolera™ Dalton was used to fractionate completely non-UV absorbing starting materials and products from a classic reductive amination reaction. A resin bound cyanborohydride reagent was used to greatly facilitate the work flow by eliminating the need for traditional work-up, and the mixture applied to flash chromatography purification methods.