Part No: AN108Issued year: 2015File size: 0.38mbFile type: pdf
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Part No: AN109Issued year: 2015File size: 0.71mbFile type: pdf
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Part No: AN077Issued year: 2013File size: 1.35mbFile type: pdf
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Part No: P098Issued year: 2014File size: 0.28mbFile type: pdf
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.
Part No: AN085Issued year: 2014File size: 0.92mbFile type: pdf
Mass-directed flash purification is a simple and effective
technique to detect and fractionate compounds with poor UV chromophores. In this study, fatty acid methyl ester mixtures were purified by mass-directed flash purification using the Biotage Isolera™ Dalton System.
Part No: P099Issued year: 2014File size: 0.39mbFile type: pdf
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Part No: P168Issued year: 2017File size: 0.28mbFile type: pdf
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Part No: PN38Issued year: 2011File size: 0.17mbFile type: pdf
Mephedrone (MCAT) is a ‘designer drug’ synthesised to mimic the amphetamine class of compounds. Due to its perceived ‘ecstasy like’ effects of euphoria, excitement and alertness combined with ease of availability, this (until recently) unregulated drug (legal high) has found widespread abuse in the UK in recent years. Harmful effects associated with mephedrone are still being studied but after several deaths were linked to the abuse of the drug, the UK Government made this a Class B substance. Mephedrone extraction was evaluated on three mixed mode cation exchange SPE sorbents: EVOLUTE® CX, EVOLUTE® WCX and ISOLUTE® HCX.
DOA, ISOLUTE, EVOLUTE, Amphetamine
Part No: Issued year: 2011File size: 0.44mbFile type: pdf
User Report: Metal scavenger Si-Thiol. Nippon Shinyaku Co., Ltd use Biotage metal scavengers to control residual metals such as palladium, which are difficult to remove by other methods. We have been talking to Mr. Toshio Fujiwara who is in charge of Process Chemistry, CMC Research & Development Department, Discovery Research Laboratories.
Part No: P148Issued year: 2016File size: 0.93mbFile type: pdf
Biotage metal scavengers are a more effective alternative to traditional carbon batch adsorption processes. They have the lowest metals content, and also excellent extractable profiles, therefore not likely to risk contamination, unlike competitive materials we tested. Furthermore, Biotage metal scavengers offer volumetrically, a much more compact solution than carbon, requiring 48 times less volume for the same degree of Pd removal in our studies.