Part No: PPS437Issued year: 2016File size: 0.19mbFile type: pdf
Biotage® SNAP Bio flash cartridges were developed with a small
particle size (20 μm) and large pore size (300 Å) to provide
increased resolution and effective separation of complex
peptide mixtures. Depending on the quality of the peptides
synthesized, flash chromatography can be used either as the
sole method of purification or as a front end clean-up prior to
SNAP Bio offers close to prep-HPLC performance in a flash
format at a fraction of the cost
Part No: TN-0016.0108Issued year: 2008File size: 0.4mbFile type: pdf
Biotage SNAP cartridges can be used on any flash system.
The easy-to-use Luer-lok® connections do not need a
compression module to operate at 100 psi. Made from USP
Class VI materials, Biotage SNAP cartridges offer 7 types of
loading techniques including 3 internal dry loading options.
Packed to provide the highest plate count of any 50μm particle
cartridge on the market, SNAP provides increased loading
capacity and better compound separations.
Part No: TN-0025.0508Issued year: 2008File size: 0.35mbFile type: pdf
SNAP KP-NH cartridges contain amine functionalized silica.
This optimized media shields synthetic organic amines from
acidic silanols providing improved selectivity, peak shape,
purity and yield (Figure 1). Unlike traditional silica and 1°
amine (propyl amine) bonded silica, KP-NH doesn’t require the
use of chlorinated solvents or amine additives.
Part No: Issued year: 2009File size: 0.06mbFile type: pdf
SNAP flash cartridges provide several sample load options including both internal and external liquid and dry loading. This
Quick-start Guide provides sample load guidelines and loading technique suggestions, which should help you get the most
from your SNAP cartridges.
Part No: Issued year: 2008File size: 0.09mbFile type: pdf
SNAP flash cartridges provide several sample load options including both internal and
external liquid and dry loading. This Quick Start Guide provides sample load guidelines and
loading technique suggestions, which should help you get the most from your SNAP
Part No: TN-SNAP-RPC-0211Issued year: 2011File size: 1.11mbFile type: pdf
Reversed-phase flash chromatography is a very popular
purification technique using a non-polar stationary phase.
Main application areas include separation of polar, ionizable
and highly lipophilic compounds which cannot easily be
separated by normal-phase techniques.
Part No: TN-0032.0112Issued year: 2012File size: 0.75mbFile type: pdf
Precision engineered Biotage SNAP Ultra cartridges deliver double
the purification capacity utilizing small particle spherical silica with
an 40% increase in surface area. This proprietary silica reduces
peak width and provides higher concentration fractions with less
Part No: PPS447Issued year: 2017File size: 0.96mbFile type: pdf
Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing,
SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Part No: PPS425Issued year: 2016File size: 2.58mbFile type: pdf
Biotage® V-10 Touch rapidly dries samples dissolved in both aqueous and organic solvents. Combined with the Gilson liquid handling robot automatically transfers your purified samples to V-10 Touch for sequential automated evaporation. This brochure lists all evaporation vials, adapters and accessories.
Part No: UI303Issued year: 2013File size: 4.69mbFile type: pdf
The Biotage® VacMaster™ is a state-of-the-art sample processing station. It incorporates innovative design features that make simultaneously processing multiple samples easier than ever before. For scientists working with biological fluids, these features and operating procedures provide a safe system.
Part No: UI327Issued year: 2015File size: 1.4mbFile type: pdf
Biotage® ACT Plate Adapter (patent pending) is designed to reduce or eliminate the occurrence of well-to-well cross contamination (cross talk) during extract evaporation, and fits on top of the 96-well collection plate during evaporation.
Part No: TN0151611_01Issued year: 2016File size: 0.35mbFile type: pdf
To improve upon the original SmartPrep Cartridge Extractor’s design, the SmartPrep Cartridge Extractor II includes a Ceramic Seal in each valve rather than a Rulon® Seal. These Ceramic Seals were specially selected to increase the durability of the valves with tough sample matrices, including those that may be abrasive.
Part No: Issued year: 2012File size: 0.11mbFile type: pdf
The following organization’s Quality Management System has been assessed and registered by Intertek Certification AB as conforming to the requirements of: SS-EN ISO 9001:2008
The Quality Management System is applicable to: Development and production of specialty polymers (MIPs and NIPs) for industrial separations and laboratory trace analysis.
Part No: CM-INT-0810Issued year: 2010File size: 0.25mbFile type: pdf
ChemMatrix is a patented, 100% PEG (polyethylene glycol)
based resin developed by PCAS BioMatrix that offers substantial
advantages over traditional PEG based polystyrene resins for
solid phase peptide synthesis.
Part No: RT-CM-0111Issued year: 2010File size: 0.09mbFile type: pdf
In order to keep your RapidTrace® workstation clean, in good working order and free from contamination it is of course essential to
routinely clean and purge the fluid path. Whichever matrix and reagents are running through the system, traces of these liquids will
be left after use; proteins from plasma, precipitates from buffers or even particulates from oral fluid. Over time if the following
cleaning schedule is not observed these contaminants could cause blockages leading to malfunctions and/or cross contamination.
Part No: PPS451Issued year: 2017File size: 2.99mbFile type: pdf
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Part No: P136Issued year: 2015File size: 0.6mbFile type: pdf
This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared.
MSACL EU 2015
Part No: AN022-HORIssued year: 2009File size: 0.91mbFile type: pdf
EPA Method 1657 describes the procedure to determine low ppb levels of organophosphorous pesticides in wastewater.
The disks compared in this study are: “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm).
Part No: AN018-HORIssued year: 2010File size: 0.9mbFile type: pdf
EPA Method 608 describes the procedure to determine low parts per billion levels of organochlorine pesticides, polychlorinated biphenyls, toxaphene and chlordane in wastewater. The laboratory that collected the data presented in this application note uses EPA method 608 ATP3M0222 as the approved SPE technique for waste water extraction.
The disks compared in this study are : “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm). Comparisons were made using side-by-side extraction and identical calibration parameters.
Part No: AN008-HORIssued year: 2009File size: 0.89mbFile type: pdf
EPA Method 525.2 describes the procedure to determine
low ppb levels of semi-volatile organic material in drinking
water using solid phase extraction (SPE) or liquid-solid
extraction (LSE) techniques. The extraction solvents are
methylene chloride and ethyl acetate. Extracts are analyzed
by GC/MS using a splitless injection technique.
Part No: PPS362Issued year: 2014File size: 1.23mbFile type: pdf
This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled
stripped plasma using a supported liquid extraction procedure.
Part No: PPS366Issued year: 2014File size: 1.76mbFile type: pdf
Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: PPS361Issued year: 2014File size: 1.28mbFile type: pdf
This document compares automated sample preparation using the Biotage®
Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.
Part No: P177Issued year: 2018File size: 0.59mbFile type: pdf
This poster compares sample preparation options (solid phase extraction using EVOLUTE EXPRESS ABN, and supported liquid extraction using ISOLUTE SLE+) for the extraction of a panel of endogenous steroids from serum.
MSACL NA 2018, Palm Springs, CA
ASMS 2018, San Diego, CA
Part No: P142Issued year: 2016File size: 0.57mbFile type: pdf
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
Part No: AN023-HORIssued year: 2012File size: 0.85mbFile type: pdf
The purpose of this application note is to ensure that your environmental laboratory is in compliance with EPA Method 1664A Modifications or EPA Method 1664B utilizing the Horizon Technology SPE-DEX® 1000XL/3000XL Controller with application firmware version 1.08.
Part No: AN024-HORIssued year: 2012File size: 0.86mbFile type: pdf
The purpose of this application is to demonstrate the viability of a solid phase extraction (SPE) method utilizing the Horizon Technology SPE-DEX 1000/3000XL Controller with application firmware version 2.2 to extract samples for EPA Method 1664A/B and fulfill all QC requirements.
Part No: AN879Issued year: 2017File size: 0.89mbFile type: pdf
There are a wide range of volatile and semi-volatile contaminants finding their way into both terrestrial bodies and water sources worldwide. In the United States (US), the contaminants are analyzed
according to stipulated US-EPA methods. In the European Union (EU), a large number of these same compounds are tested according to the European Water Framework Directive. Though these analytes are approached differently from a regulatory perspective, it is clear that background monitoring occurs on a global basis. Initial extraction of these analytes depends on the matrix being analyzed and is often a multifaceted process, but ultimately analysts are presented with some form of extraction/organic solvent they must concentrate to achieve instrumental limits of quantification. Presented within this technical note are the results of such an evaporative process using the new Biotage TurboVap® II.
Part No: TN0031405_01Issued year: 2014File size: 0.74mbFile type: pdf
Although the general analysis process contains extraction, extract drying, and evaporation steps prior to chromatography, this note will describe a procedure for the concentration of organic solvent extracts containing phenolic compounds.
Part No: PPS430Issued year: 2016File size: 0.97mbFile type: pdf
At the Forensic Medicine Lab at Toho University, researchers use ISOLUTE® SLE+ columns from Biotage. When dealing with samples that easily form emulsions like urine or blood, it allows researchers to use the established liquid-liquid extraction technique, saving significant amount of time on analysis. We spoke with the Head of the Forensic Medicine Lab Professor Masaru Terada.
Part No: PN0081601_1Issued year: 2016File size: 0.67mbFile type: pdf
The SPE-DEX 3100 Extractor is controlled through a small hand-held controller tethered to the instrument. The color touch screen is easy to see from any angle and features large icons to show the status of each station in the unit at any time.
Part No: Issued year: 2009File size: 0.12mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB and a number of acquisitions were performed within the Medicinal Chemistry sector during the years of 2003-2005. After divesting the business area Biosystems in October 2008, the Company’s business is today made up entirely of the business area Discovery Chemistry. Biotage’s headquarters is located in Uppsala.
Part No: Issued year: 2010File size: 0.2mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (”the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2011File size: 0.72mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (“the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2013File size: 0.11mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company is mainly active within analytical and organic chemistry.
Part No: PPS445Issued year: 2017File size: 0.62mbFile type: pdf
Ease of use is what stands out as the top feature of Isolera™ flash chromatography
system for Professor Anna Bernardi, head of the synthetic organic chemistry
research group at the University of Milan. Her current research goal: developing
sugar-like molecules, called glycomimetics, for healthier life.
Part No: PPS443Issued year: 2017File size: 2.31mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: Issued year: 2011File size: 0.07mbFile type: pdf
Through innovative patented technology based on molecularly imprinted polymers (MIPs)
you will experience improved clean up in your processes. With a high probability of success,
unwanted contaminants or high value desirables can be selectively extracted from your
processes, resulting in more efficient production and cleaner products.
Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: AN012-HORIssued year: 2010File size: 0.99mbFile type: pdf
The second Unregulated Contaminant Monitoring Regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 529 is categorized under List 1, Assessment Monitoring in the UCMR2 program, and focuses on three explosives: 1,3-dinitrobenzene; 2,4,6- trinitrotoluene (TNT); and hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX). The resulting data will be used by the EPA to determine whether or not regulatory standards should be established for these compounds.
Part No: AN009-HORIssued year: 2015File size: 1.33mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN019-HORIssued year: 2010File size: 0.92mbFile type: pdf
The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.
Part No: AN021-HORIssued year: 2009File size: 0.89mbFile type: pdf
Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater.
The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk.
The Biotage® Horizon 4790 Automated Extraction System with Envision®
Platform controller, and the DryVap® Automated Drying
and Concentration System were used in this application note.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: Issued year: 2014File size: 0.12mbFile type: pdf
This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme.
MSACL EU 2014
Part No: AN004-HORIssued year: 2009File size: 0.72mbFile type: pdf
This evaluation study was conducted to determine the feasibility of the Biotage SPE-DEXÒ 4790 Automated Extraction System for the extraction of herbicides and pesticides. The preliminary results demonstrate the capability of this method for the automated SPE extraction of organic compounds.
Part No: Issued year: 2017File size: 0.27mbFile type: pdf
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX).
Part No: AN057Issued year: 2012File size: 0.21mbFile type: pdf
The formation of diarylethers by reacting an arylhalide and phenol is usually a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield. The substitution patent of the electrophile and the nucleophile affects the reaction times mostly. A sterically hindered electrophile and a strongly deactivated nucleophile as outlined in the (Scheme 1) below, gives a very low yield (13 %) at conventional reflux for 2 weeks.1,2 Remainder was
recovered starting material. We have previously reported the dramatically shortened reaction time to 1 hour along with improved yield running the reaction outlined in the Scheme by heating by microwaves.
Part No: P131Issued year: 2015File size: 0.47mbFile type: pdf
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Part No: P171Issued year: 2017File size: 0.69mbFile type: pdf
This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids.
SOFT 2017, Boca Raton
Part No: P174Issued year: 2017File size: 1.44mbFile type: pdf
This poster discusses the potential for a single extraction protocol
for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis.
SOFT 2017, Boca Raton
also presented at SFTA, Marseille, France, 2018
Part No: P157Issued year: 2017File size: 0.8mbFile type: pdf
This poster demonstrates that a large urine panel, comprised of 43 DOAs, from multiple drug classes, can be simultaneously screened by mixed-mode cation exchange SPE (using EVOLUTE EXPRESS CX 96 well plates) despite their disparate intermolecular traits, by thoughtfully selecting appropriate organic wash and elution conditions that simultaneously enable sample isolation and detection along with minimizing sample matrix effects.
The extraction method is automated using the Biotage® Extrahera™ Automated sample Preparation Platform.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Part No: DLV_TN.0111Issued year: 2011File size: 0.08mbFile type: pdf
One of the most common flash purification challenges is
dealing with hard-to-dissolve crude samples. Because polar
solvents cause poor chromatographic results when used as
injection solvents in normal-phase flash chromatography, other
sample load options are needed.
Part No: F0221702_01Issued year: 2017File size: 0.62mbFile type: pdf
The DryDisk membrane drying system provides advantages for removing water from nonpolar solvents, important in protecting the chromatograph in the analysis step, especially critical in protecting GC and GC/MS.
Part No: TN0081507_01Issued year: 2015File size: 0.55mbFile type: pdf
Removing water with a membrane rather than the older technique of passing the solvent through a column of sodium sulfate (Na2SO4) brings several advantages. The most important analytically is that the membrane will not adsorb analytes or contaminate the extract with matrix or other potential interferences.
Part No: Issued year: 2006File size: 0.46mbFile type: pdf
Why spend hours using traditional evaporation techniques when the automated evaporation capability of the DryVap System can take your compound to
dryness in minutes? Through the precise application of vacuum, heat and nitrogen sparge, the DryVap System gently and predictably evaporates all residual solvent from your compound allowing you to quickly move on to the next step.