Part No: P155Issued year: 2017File size: 0.61mbFile type: pdf
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
Part No: P152Issued year: 2016File size: 0.49mbFile type: pdf
This poster describes a method for the simultaneous extraction (using EVOLUTE EXPRESS WCX) and analysis of plasma catecholamines and metanephrines. A highly sensitive, linear and rugged assay was developed, and automated using the Biotage Extrahera sample preparation processing system.
MSACL EU 2016
Part No: AN755Issued year: 2012File size: 1.03mbFile type: pdf
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats.
EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,
Part No: AN098Issued year: 2014File size: 0.81mbFile type: pdf
To demonstrate the cost savings by employing small μmol scale automated solid phase peptide synthesis through robot liquid handling of reagents with accurate dispensing of small volumes, a 10-mer antimicrobial peptide was synthesized.
Part No: Issued year: 2008File size: 0.13mbFile type: pdf
The adapter kit contains a pre-assembled stand, spacer and
connecting tubing; everything required to allow the use of
SNAP 340g cartridges on flash purification systems that utilize
Luer tubing connections such as, Isco Companion and Analogix
Part No: install_snap_25.zipIssued year: 2012File size: 0mbFile type: zip
7742_install_snap_25.zip. SPX 25 g SNAP cartridge installation.
The system already has the SNAP 10 g, 50 g, 100 g and 340 g cartridges installed
(only the SNAP 25 g needs to be installed)
Part No: install_snap_full.zipIssued year: 2012File size: 0mbFile type: zip
install_snap_full.zip. SNAP Software Update for Biotage® SP Systems. SPX FULL SNAP cartridge installation instructions.
The systems that does not have any SNAP cartridges installed
(the SNAP 10 g, 25 g, 50 g, 100 g and 340 g will be installed)
Part No: TN-SNAP XL-0211Issued year: 2011File size: 1.22mbFile type: pdf
The SNAP XL cartridges address a growing need for optimized gram- to ~100+ g-scale purification, increasing efficiency and reducing costs. These cartridges extend the
SNAP product ranges, providing chemists with more scalable tools for their synthesis and purification needs. There are 3 members in this family of cartridge solutions, KP-Sil for normal phase, C18 for reverse phase and KPNH for more difficult normal phase systems, such as amines. SNAP XL cartridges will allow you to save hours, even days of purification run-time.
Part No: TN114Issued year: 2006File size: 0.12mbFile type: pdf
New approaches to the quantitation of drugs and metabolites in biological fluids have increased the need for high throughput methodologies to avoid the traditional laboratory bottleneck of sample preparation. Solid phase extraction in a 96-well plate format such as ISOLUTE-96 Fixed Well Plates or ISOLUTE Array 96-well Plates is a solution to this problem.
Part No: Issued year: 2013File size: 2.08mbFile type: pdf
Amide-linked homopolymers of sialic acid offer the advantages of stable secondary structure and increased bioavailability making them useful constructs for pharmaceutical design and drug delivery. Defining the structural characteristics that give rise to secondary structure in aqueous solution is challenging in homopolymeric material due to spectral overlap in NMR spectra. Having previously developed computational tools for heteroologomers with resolved spectra, we now report that application of these methods in combination with circular dichroism, NH/ND NMR exchange rates and nOe data has enabled the structural determination of a neutral, d-amide-linked homopolymer of a sialic acid analogue called Neu2en.
Keywords: sialic acid; Neu2en; NMR; circular dichroism; helical peptide; water-soluble scaffold; blood serum; proteolysis; 1,3-dipolar cycloaddition chemistry; click chemistry; Initiator+ SP Wave
Jonel P. Saludes, Travis Q. Gregar, I. Abrrey Monreal, Brandan M. Cook, Lieza M. Danan-Leon, Jacquelyn Gervay-Hague
Part No: Issued year: 2007File size: 0.22mbFile type: pdf
Biotage has created a new Resin-based Chemistry Kit that contains a variety of resins
designed to enable a choice of key resin-assisted chemistries with optimized protocols
(Figure 1). Reductive amination, coupling (C-C or C-O bond formation), amide bond
formation and oxidation are widely used traditional chemical transformations you’ll be able
to perform with this new kit.
Part No: TN-0021.0207Issued year: 2007File size: 0.34mbFile type: pdf
Resins are usually described as one of 2 main types, Gel (PS) or macroporous (MP).
The gel resins have a low cross-linking and need to swell to allow access to their
functional groups. Therefore, they are very effective in transformations requiring
swelling organic aprotic solvents such as DCM, THF, Dioxane etc. Conversely, MP
resins are more highly cross-linked with more of a permanent open pore structure,
so they do not require swelling in such solvents for effective performance. As a
result, MP resins are compatible with a wider range of solvents than PS resins and
perform better in solvents such as water, MeOH and MeCN. Resins are generally
stable and can be stored indefinitely under cool dry conditions. For longer term
storage standard refrigeration (4 °C) is recommended.
Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications.
Keywords: turbovap, turbo vap, v10, v-10, spedry, spe dry, evaporation, concentrat