Part No: AN857Issued year: 2016File size: 2.29mbFile type: pdf
This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection.
A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Part No: Issued year: 2011File size: 0.16mbFile type: pdf
Molecularly Imprinted Polymers (MIPs) are highly cross-linked polymers with selective binding sites engineered to contain recognition elements in defined positions1. MIPs have been developed for a large variety of small molecules, peptides, carbohydrates and even proteins. The schematic 0of a MIP site.
Part No: P055Issued year: 2013File size: 0.7mbFile type: pdf
Free urinary cortisol levels can be an indicator of a variety of disorders, such as Cushing Syndrome. This poster presents a simple method for the extraction of cortisol from urine demonstrating good recoveries and low ion suppression. LC-MS/MS analysis was performed using a Waters 2795 Liquid Handling Unit coupled to a Quattro Ultima Pt triple quadrupole MS using electrospray ionization, operated in the MRM mode. Supported liquid extraction method development resulted in recoveries greater than 90% for cortisol spiked urine. Calibration curves constructed using this method from 25-2000 ng/mL, demonstrated excellent linearity with coefficients of determination greater than 0.99.
Cortisol, Stress, Cushin's Syndrome, SLE, Supported Liquid Extraction, SPE, LLEMSACL, San Diego, 2013
Part No: Issued year: 2004File size: 0.39mbFile type: pdf
•Metal-mediated [2 + 3] cycloaddition to coordinated nitriles
-- synthesis of new transition metal complexes
-- synthesis of new heterocycles
-- enantioselective synthesis of heterocycles
role of microwaves?
• Platinum-catalysed cycloisomerisation of alkynes
-- Synthesis of Taxol-like compounds
-- highly efficient and atom economic chemistry
role of microwaves?
Part No: AN061Issued year: 2012File size: 0.63mbFile type: pdf
Acetone is successfully used as solvent in normal-phase flash chromatography when used with an Isolera™ Spektra flash purification system. The new λ-All detection and baseline correction features provide compound detection at all wavelengths in the detector’s range while minimizing any baseline drift due to solvent UV absorption.
Part No: PPS410Issued year: 2015File size: 0.7mbFile type: pdf
User Case: Professor Roger Strömberg, Karolinska Institutet. One of Professor Strömberg’s projects at Karolinska Institutet
involves the synthesis of artificial nucleases and PNAzymes. In
2013, his research group acquired a Biotage® Initiator+ Alstra™
peptide synthesizer for making PNAs and peptides.
Part No: PPS409Issued year: 2015File size: 1.61mbFile type: pdf
RIKEN is Japan’s largest research organization with 3,400
researchers from more than 50 countries. The chemists in RIKEN’s
Sugiyama Laboratory use EVOLUTE® EXPRESS solid-phase extraction
plates from Biotage in the analysis of clinical trial samples
for pharmacokinetic studies.
Part No: PPS432Issued year: 2016File size: 0.4mbFile type: pdf
Customer Case: Hitachi, Ltd. Healthcare Innovation Center. Hitachi’s R&D group has developed a new method for analyzing pharmacodynamics in cultured cells, in a study aimed at improving the pass rate in clinical trials. Mr. Ryosuke Takahashi tells the story.
Part No: Issued year: 2013File size: 0.43mbFile type: pdf
User report: ISOLUTE SLE+. Shin Nippon Biomedical Laboratories, Ltd. use ISOLUTE® SLE+ to prepare biological samples for pharmacokinetic testing as part of a drug discovery and development program. We had the chance to speak with Mr. Mitsuhiko Kawabata and Ms. Akiko Toda of the pharmacokinetics group.
Part No: PPS406Issued year: 2015File size: 2.25mbFile type: pdf
Tokyo based PeptiDream Inc. is a company specializing in non-standard peptide therapeutics containing non-standard amino acids, and conducts research and development of new drug candidates. PeptiDream uses the peptide synthesizers Syro I and Biotage® Initiator+ Alstra™ for the synthesis of various kinds of peptides to support drug discovery and development.
Part No: AN042Issued year: 2003File size: 0.08mbFile type: pdf
Thin-layer chromatography (TLC) is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system. The method transfer accuracy of TLC to flash is based on the TLC silica and flash silica properties. When identical, the equation 1/Rf = CV holds, where Rf is the retention factor of a compound separated by TLC, and CV is the number of column volumes required to elute a compound. Cartridge loading capacity is based on the difference in CV (DCV) between two adjacent compounds.
Part No: Issued year: File size: 0.62mbFile type: pdf
The utilization of bound reagents for the solution phase synthesis has become an important tool in synthesis of biologically active molecules. The disadvantage of this technique is the relative slow rate of reaction. Microwave irradiation has been used to overcome this problem and increase rate of reaction of solid-assisted solution phase synthesis. This presentation covers developing efficient and robust strategies for the preparation of an array of cyclic tertiary amines using microwave irradiation in conjunction with bound reagents and scavengers such as MP-cyanoborohydride.
Part No: Issued year: 2015File size: 0.94mbFile type: pdf
The crossreactivity of molecularly imprinted polymers (MIPs) and its
practical implications are discussed. Screening of MIP libraries is presented as a
fasttrack route to discovery of resins selective towards new targets, exploiting the
fact that MIPs imprinted with one type of template molecule also show recognition
to related and sometimes also to apparently unrelated molecules. Several examples
from our own and others’ studies are presented that illustrate this crossreactivity and
the pattern of recognition is discussed for selected examples.