Part No: AN705Issued year: 2010File size: 0.1mbFile type: pdf
Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase.
molecularly imprinted polymers, MIP, BIOTAGE, AFFINILUTE, NSAIDs, Environmental, SupelMIP
Part No: AN741Issued year: 2012File size: 0.6mbFile type: pdf
This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LODs as low as 500 pg/mL.
Opiates, SLE, SLE+, Supported Liquid Extraction, Urine, Oasis, Waters, SPE, Solid Phase Extraction, Sample Prep, quick, easy, DOA, Drugs of Abuse
Part No: AN866Issued year: 2016File size: 1.4mbFile type: pdf
This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of opiate compounds from oral fluid matrix collected using the NeoSal device, prior to GC/MS analysis.
Part No: AN852Issued year: 2015File size: 0.72mbFile type: pdf
This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and cocaine.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 50 ng/mL (analyte dependant).
Part No: IST1027AIssued year: 2011File size: 0.5mbFile type: pdf
This application note was developed for the clean-up of a non-polar solvent extract of vegetable material. The methodology is applicable to a wide range of organochlorine and organophosphorus pesticides and a number of vegetable types. The analytes are relatively non-polar, and will not be strongly retained by polar/anion exchange sorbents.
Part No: AN737Issued year: 2011File size: 0.27mbFile type: pdf
This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL.
resin, polymer, EVOLUTE,
Part No: AN831Issued year: 2014File size: 1.55mbFile type: pdf
The environmental persistence of pharmaceuticals is of increasing interest, with little known regarding removal during wastewater treatment. This study investigates the potential of commercially available QuEChERS kits for the extraction and clean-up of a suite of common over-the-counter (OTC) pharmaceuticals from water and sludge cake. Extracts are analysed using LC-MS/MS.
Part No: AN882Issued year: 2017File size: 1.27mbFile type: pdf
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure.
Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.
Part No: AN763Issued year: 2012File size: 0.52mbFile type: pdf
The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed to cleave glucuronide moiety and produce the free form of drug. The target analytes can them be extracted from the urine matrix using EVOLUTE CX solid phase extraction cartridges. LOQs range from 5 ng/mL to 0.5 ng/mL with recoveries above 80% and RSD <10%
Pain Management, EVOLUTE, CX, SPE, Solid Phase Extraction, Polymer, LC-MS-MS, Cocaine, Diazepam, Heroin, Morphine, Oxycodone, Fentanyl, Diazepam, Mixed Mode, RapidTrace
Part No: AN781Issued year: 2013File size: 0.29mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol for the extraction of patulin from clear apple juice using ISOLUTE Myco SPE columns, for analysis by LC-MS/MS. This method achieves high analyte recoveries with RSDs below 10%.
mycotoxin, patulin, apple juice, LCMSMS
Part No: AN750Issued year: 2012File size: 1.14mbFile type: pdf
This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant.
Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,
Part No: AN701Issued year: 2007File size: 0.07mbFile type: pdf
This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency.
Pharmaceuticals, waste water, antibiotics, EVOLUTE,
Part No: IST1019AIssued year: 2011File size: 0.14mbFile type: pdf
This method was developed for the extraction of phencyclidine from meconium for drugs ofabuse applications. The analyte has an ionisable amine group, which is exploited through cation exchange interactions. The pKa is approximately 8, so the compound is fully ionized at pH values of less than 6. The analyte also has significant non-polar characteristics, and both hydrophobic and ion exchange retention mechanisms are used to give a clean extract.
ISOLUTE, PCP, DOA, Varian Bond Elut Certify, UCT CleanScreen DAU
Part No: IST1039AIssued year: 2011File size: 0.14mbFile type: pdf
This application was developed for the extraction of phencyclidine and other basic drugs from urine for drugs of abuse applications. Typical recovery for PCP is > 85%.
PCP, DOA, Biotage, ISOLUTE, UCT CleanScreen DAU, Varian Bond Elut Certify
Part No: AN876Issued year: 2017File size: 2.77mbFile type: pdf
Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.
The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Part No: AN798Issued year: 2013File size: 1.33mbFile type: pdf
This application note describes a novel sample cleanup strategy to address the complex matrix effects associated
with commercial milk product variables while maintaining adequate analyte recovery and repeatability.
Part No: IST1025A.V.1Issued year: 2016File size: 0.66mbFile type: pdf
This application note describes the extraction of polyaromatic hydrocarbons (PAHs) from natural waters with a high concentration
of humic acids. Layered SPE columns are used to eliminate humic acids from the final extract.
Part No: AN883Issued year: 2017File size: 1.33mbFile type: pdf
The method described in this application achieves high reproducible recoveries for a number of common PFCs in water using an optimized weak cation exchange SPE procedure with EVOLUTE® EXPRESS WAX SPE columns.
Part No: P133Issued year: 2015File size: 0.51mbFile type: pdf
This poster demonstrates a simple supported liquid extraction procedure for the extraction of propofol from whole blood suitable for GC-MS analysis.
In addition, phospholipid profiles in the extracts were investigated using LC-MS/MS, and showed no breakthrough of these blood components, suggesting the procedure is appropriate for use with LC-MS/MS based analytical procedures.
Part No: AN753Issued year: 2011File size: 0.33mbFile type: pdf
Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from human serum prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from serum allowing for mass spectral detection of the target analytes at a concentration of 100 ng/mL or less.
Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Serum, Clinical, LCMS, Sample Prep
Part No: AN754Issued year: 2011File size: 0.3mbFile type: pdf
Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from whole blood prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from whole blood allowing for mass spectral detection of the target analytes at a concentration of 200 ng/mL or less.
Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Whole Blood, Clinical, LCMS, Sample Prep
Part No: AN774Issued year: 2012File size: 0.19mbFile type: pdf
This application note describes the extraction and quantitation of Cannabimimetic Naphthoylindoles (Synthetic Cannabinoids) and their metabolites (JWH Series) from various matrices using Supported Liquid Extraction (SLE). Synthetic Cannabinoids or SPICE as they are commonly known have become an increasing problem as one of the newest forms of illicit drugs being consumed today. These compounds bind to the cannabinoid receptors in mammals triggering similar euphoric symptoms as Tetrahydrocannabinoids (THC). Currently robust and fast analytical methods of analysis are required to aid in the screening and detection of this growing class of compounds. The recoveries obtained for the synthetic cannabinoids parent and metabolites ranged from 70-98 %.
Supported Liquid Extraction, SLE, SLE+, SPICE, Synthetic Cannabinoids, JWH, metabolites,
Part No: AN791Issued year: 2013File size: 3.25mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.
SPICE, oral fluid, Intercept Orasure, LC-MSMS, SLE
Part No: AN779Issued year: 2013File size: 1.2mbFile type: pdf
This application note describes the extraction of a range of synthetic cannabinoids and metabolites from oral fluid collected using the Quantisal™ Oral Fluid Collection Device prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for loading volumes of either 400 μL or 1 mL of matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs <10% for all analytes.
Part No: AN773Issued year: 2013File size: 1.25mbFile type: pdf
This application note describes the extraction of a range of synthetic cannabinoids and metabolites from whole blood prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for extraction of 800 μL of pre-treated matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs lower than 10% for all analytes.
Part No: P075Issued year: 2014File size: 0.66mbFile type: pdf
Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and
quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we
demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices.
Part No: AN780Issued year: 2013File size: 1.33mbFile type: pdf
This application note describes effective and efficient protocols optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <10% for all analytes.
Part No: AN870Issued year: 2016File size: 1.23mbFile type: pdf
This application note describes the extraction of a range of synthetic cannabinoids (SPICE) and metabolites, using ISOLUTE SLE+ supported liquid extraction columns, from oral fluid matrix collected using the NeoSal™ devices, prior to GC/MS analysis.
Part No: AN721Issued year: 2011File size: 0.3mbFile type: pdf
This application note describes the extraction of Tamoxifen and metabolites from urine using ISOLUTE SLE+ supported liquid extraction plates followed by LC-MS/MS analysis.
ISOLUTE, SLE, SLE+, Tamoxifen, Hormones, Oestrogen
Part No: AN869Issued year: 2016File size: 0.91mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol, using EVOLUTE EXPRESS ABN solid phase extraction plates, for the extraction of teicoplanin from plasma prior to HPLC-DAD analysis.
Part No: AN740Issued year: 2011File size: 0.38mbFile type: pdf
This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL.
ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL
Part No: AN809Issued year: 2014File size: 1.3mbFile type: pdf
This application note describes the simultaneous extraction of THC and its major metabolites, including 11-nor-9-carboxy-Δ9-THC glucuronide, from urine using supported liquid extraction (ISOLUTE® SLE+ in both plate and column formats) prior to analysis by LC-MS/MS.
Part No: IST1035Issued year: 2011File size: 0.11mbFile type: pdf
This method was developed for the extraction of the tetrahydrocannabinol carboxylic acid metabolite (THC-COOH) from urine using a mixed non-polar and anion exchange retention mechanism. Typical recoveries of the analyte are > 75%.
THC, THC-COOH, BIOTAGE, ISOLUTE, SPE, DOA, drugs of abuse
Part No: AN867Issued year: 2016File size: 1.28mbFile type: pdf
This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of THC, THCA and Carboxy-THC from oral fluid matrix collected using the NeoSal device, prior to GC/MS analysis.
Part No: AN840Issued year: 2015File size: 0.7mbFile type: pdf
This application note describes the extraction of Δ9-THC, 11-hydroxy- Δ9-THC and 11-nor-9-carboxy-THC from whole blood matrix, prior to GC/MS analysis.
An extremely simple sample pre-treatment and extraction method is employed to extract the parent THC and main metabolites from complex whole blood matrix, delivering high, reproducible analyte recoveries.
Part No: AN819Issued year: 2014File size: 1.54mbFile type: pdf
This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to GC/MS analysis.
Part No: AN822Issued year: 2014File size: 1.57mbFile type: pdf
This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Quantisal™ (Immunalysis) device, prior to GC/MS analysis.
The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 75% with RSDs lower than 10% for all analytes.
Part No: AN881Issued year: 2017File size: 1.57mbFile type: pdf
The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 200 μL of serum was extracted using the EVOLUTE® EXPRESS AX 30 mg fixed well plate format. High reproducible recoveries and low phospholipid content were observed, demonstrating limits of quantitation < 50 pg/mL.
Part No: AN884Issued year: 2017File size: 0.62mbFile type: pdf
TSNAs, or tobacco-specific nitrosamines, are carcinogens found in tobacco products, including e-cigarettes and smokeless tobacco. This proof of concept study focusses on NNN (n-nitrosonornicotine), but the supported liquid extraction methodology described is a also suitable for NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone) and NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol).
Part No: AN716Issued year: 2011File size: 0.14mbFile type: pdf
The following method has been developed for the extraction of the following triazines and triazine metabolites; atrazine, simazine, propazine, cyanazine, sebuthylazine deisopropylatrazine, deethylatrazine, deethylterbutylazine, prometon and hydroxyterbutylazine from water. Recoveries are in the range 80-95% and are reproducible, allowing sharp decision limits. The maximum accepted level of triazines in drinking water is 0.1 μg/L (EU legislation).
AFFINILUTE, MIP, SupelMIP, Molecularly Imprinted Polymer
Part No: AN601Issued year: 2008File size: 0.12mbFile type: pdf
This Application Note describes the development of an automated procedure for high throughput supported-liquid extraction of three tricyclic antidepressant drugs from human plasma, using the ISOLUTE® SLE+ Supported-liquid Extraction Plate. Analyte recovery, along with the speed and efficiency are compared to traditional LLE.
Biotage, Extralute, Ostro, supported liquid extraction, liquid liquid extraction,
Part No: AN712Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of tobacco specific nitrosamines (NNN, NNK, NAT, and NAB) from human urine. The method is highly reproducible and offers an average recovery of 80% (95% for NNK and NAT; and 70% for NNN and NAB). Detection limits of 4 pg/mL are readily achieved for urine samples using LC-MS-MS analysis.
MIP, SupelMIP, Molecularly Imprinted Polymer,
Part No: AN871Issued year: 2017File size: 3.46mbFile type: pdf
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from urine prior to LC-MS/MS detection.
Included in this application note are the detailed settings for implementing the method on the Biotage® Extrahera(TM) automation system.
Part No: P145Issued year: 2016File size: 0.42mbFile type: pdf
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
Part No: AN880Issued year: 2017File size: 3.38mbFile type: pdf
The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
Part No: AN757Issued year: 2012File size: 1.61mbFile type: pdf
Vitamin D deficiency can result in a variety of health issues such as osteoporosis, liver and kidney problems, it is often associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D monitoring is on the rise and of significant clinical relevance. This method describes the use of ISOLUTE SLE+ plates for the efficient and simple extraction of the vitamin D metabolites 25-OH vitamin D2 and 25-OH vitamin D3. Incorporated into the procedure is an integral protein binding disruption step which maximizes analyte recovery and eliminates the need for any offline protein disruption. This method has been internally validated using DEQAS approved serum samples and deuterated internal standards the results obtained are better than that required for criteria for acceptable performance for
DEQAS approval. All recoveries were consistently greater than 90% with all RSDs < 10% at LOQs of 1-3.75 ng/mL.
Vitamin, Vitamin D, VitaminD, D, Clinical, SLE+, SLE, ISOLUTE, Metabolites, Supported Liquid Extraction, 25 Hydroxy Vitamin D,
Part No: AN706Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed for the selective extraction of beta-agonists from biological matrices. Methods have been developed for both biological tissues (e.g. bovine muscle) and fluids (e.g. urine). The methods are highly reproducible and offer β-agonist recoveries in the range of 35-90%.
MIP, Molecularly Imprinted Polymers, BIOTAGE, AFFINILUTE, SupelMIP,
Part No: Issued year: 2013File size: 0.52mbFile type: pdf
Developed in collaboration with the OC Crime Lab, this poster describes the extraction of 25 benzodiazepines and sedatives from various matrices using ISOLUTE SLE+ Supported Liquid Extraction columns. This poster was presented at SOFT 2013.
Part No: Issued year: 2010File size: 1.23mbFile type: pdf
User report: Initiator. Prof. Nakamura at the Gakushuin University has described the Initiator as an essential tool for his group’s cutting-edge research. We asked Prof. Nakamura for his views on the latest trends in the fields of organic synthesis and drug discovery.
Part No: P049Issued year: 2012File size: 0.27mbFile type: pdf
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Part No: AN903 (2)Issued year: 2004File size: 0.14mbFile type: pdf
The rapid synthesis of the non-steroidal anti-inflammatory agent Fenclofenac (2-(2,4-dichlorophenoxy)phenyl acetic acid) under microwave irradiation is reported. Modified reaction conditions of traditional Ullmann ether coupling resulted in high yields of the diaryl ether. The Willgerodt–Kindler transformation and subsequent hydrolysis of thioamide resulted in 52%
overall yield of Fenclofenac.
Part No: AN806Issued year: 2013File size: 1.03mbFile type: pdf
The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step.
A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
Part No: AN794Issued year: 2013File size: 1.01mbFile type: pdf
This application note describes a method of extraction for metanephrine and normetanephrine from pooled
plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction
technology has been developed to reduce the time
associated with sample preparation methods by eliminating
traditional steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
Part No: AN795Issued year: 2013File size: 1.09mbFile type: pdf
This application note describes a method of extraction for metanephrine and normetanephrine from synthetic
urine using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction technology has been developed to reduce
the time associated with sample preparation methods by
eliminating steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for
Part No: AN080Issued year: 2013File size: 0.26mbFile type: pdf
This application note describes a method for sample preparation
of different matrixes prior to amino acid analysis according to IS
ISO 13903:2005 or the similar AOAC Official Method 994.12 using
Biotage® Initiator+ to speed up the process.
Part No: PPS436Issued year: 2016File size: 0.4mbFile type: pdf
Sanford Burnham Prebys Case Study.
Fewer transfers and the ability to evaporate difficult solvents right in
the scintillation vial has made Biotage® V-10 the go-to evaporator at
Sanford Burnham Prebys (SBP), a top medical research institute.
Part No: TN-0012.0806Issued year: 2008File size: 0.09mbFile type: pdf
Biotage FLASH 400™ Production-Scale System The FLASH 400 is a complete skid-mounted system designed for large-scale flash chromatography and adsorption purification. The FLASH 400 uses prepacked cartridges and radial compression for performance and reliability. Built with industrial-grade components that are appropriate for operations under FDA regulations and cGMP standards, the FLASH 400 is rapidly becoming the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.
Part No: AN059Issued year: 2012File size: 0.63mbFile type: pdf
Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.
Part No: AN060Issued year: 2012File size: 1.02mbFile type: pdf
Isolera Spektra provides definitive fraction purity assessment
in both 2-D and 3-D prior to any post flash analysis. With this
information chemists quickly know if a fraction contains a pure
product suitable for further mass and structure confirmation
or whether the impure fractions must be re-purified. This
knowledge improves synthesis throughput and avoids any
embarrassment of submitting impure fractions for analytical
Part No: AN092.v1Issued year: 2014File size: 1.12mbFile type: pdf
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.
Part No: AN095Issued year: 2014File size: 0.65mbFile type: pdf
Elution of polar molecules in flash chromatography sometimes requires solvent additives, making purification more cumbersome. The Isolera™ family of flash purification instruments and Biotage® SNAP KP-NH cartridges significantly reduce the extra work involved.
Part No: TN-0006.0806Issued year: 2006File size: 0.86mbFile type: pdf
Purify up to 400 grams of compound at 1 L/min with the Biotage Flash 150™ system, up to 2x faster than traditional glass columns. This robust stainless steel system safely operates at 100 psi enabling high flow rates and the use of higher viscosity solvents. A variety of cartridge media provides
chemists with selectivity choices for optimal purification conditions. Simple and reliable, this system contains everything needed to begin your separations.
Part No: TN407.1107Issued year: 2007File size: 0.07mbFile type: pdf
FlashVac-10 and -20 Sample Processing Manifolds are used
to process standard Luer tipped ISOLUTE work-up columns.
Constructed from glass or high density polyethylene, the
FlashVac manifolds are compatible with commonly used
reaction and work-up solvents.
Part No: AN086.v2Issued year: 2014File size: 1.02mbFile type: pdf
A novel resin from MIP Technologies, RENSA® PY, is able to fractionate colored compounds present in beetroot juice using mild and purely aqueous elution conditions without the use of salts or organic solvents.
Part No: Issued year: 2015File size: 1.88mbFile type: pdf
User Report: Isolera™ Dalton, Okayama University. The laboratory of Prof. Hiroyuki Miyachi at Okayama University installed Biotage’s Isolera Dalton, an automated mass-directed purification system for flash chromatography, allowing the fractionation and collection of optical isomers. “I felt like the world’s first mass-detection automated purification system had finally arrived.”
Part No: AN792Issued year: 2013File size: 2.3mbFile type: pdf
This application note describes fully automated liquid handling of the extraction of a range of drugs from urine, which are typically screened for forensic toxicology panels, using ISOLUTE® SLE+ 96-well supported liquid extraction plates.
drugs of abuse, TECAN, automated, ISOLUTE SLE+
Part No: P150Issued year: 2016File size: 0.39mbFile type: pdf
Interest in peptide-based therapeutics has risen dramatically over the last decade. More specifically, significant energy has been dedicated to cyclic peptides, their synthesis, their biological activity, and their biological stability as potential alternatives to their linear counterparts. However, synthesizing a cyclic peptide creates significant challenges not encountered in linear peptide synthesis.
Herein we present a synthesis of oxytocin describing optimization for fully automated, on-resin disulfide bond formation utilizing Branches™, a unique feature enabling visualization and specific programing for cyclic and branched peptide synthesis. We will highlight the transferability of this optimized synthesis to larger scale syntheses and conclude with the fully automated synthesis of cyclized oxytocin incorporating an isotopically labeled Leu residue, enabling use as an internal standard in MS experiments.
Part No: TN113Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
Part No: P024Issued year: 2008File size: 1.9mbFile type: pdf
It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating many of the tedious liquid handling issues associated with LLE.
Part No: TN116Issued year: 2006File size: 0.07mbFile type: pdf
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Part No: TN129Issued year: 2005File size: 0.07mbFile type: pdf
The use of ISOLUTE HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The ISOLUTE HCX-Q sorbent utilizes a combination of weak cation exchange and C8 non-polar retention mechanisms.
Part No: Issued year: 2014File size: 1.02mbFile type: pdf
Testing for drugs of abuse in oral fluids can strongly benefit the criminal justice field as a less invasive and cost-effective approach for drug detection when compared to blood or urine sampling. Oral fluid analysis has facilitated laboratory analysis for many drugs of abuse and is a constantly evolving analysis procedure which benefits from increasingly sensitive methods of detection. The Laser Diode Thermal Desorption (LDTD) source combined with Mass spectrometry is presented as a new screening tool for drug analysis in Oral Fluids. Analysis speed of LDTD provides accurate results in seconds in combination with exceptional specificity of MS instruments make a powerful platform for the screening of different drugs of abuse and new emerging drugs. Different extraction procedures are available; however those methods depend on specific drug conditions: basic or acid drugs / hydrophilic or hydrophobic. A new extraction approach, Supported Liquid Extraction (SLE+) is evaluated as generic extraction procedure.
Part No: AN056Issued year: 2012File size: 0.2mbFile type: pdf
Quinolines are an important class of broad-spectrum antibiotics1 that were traditionally obtained by refluxing an aniline and diethyl ethoxymethylemalonate (Scheme 1) for several hours often in low yield.2 Heating the reaction mixture by microwaves to temperatures above the boiling points, with or without solvents, improves the yields and shortens the reaction time dramatically.3-6
Part No: AN106Issued year: 2015File size: 0.3mbFile type: pdf
The term “Green Chemistry” has become a major part of the
science community’s lexicon. In this application note we will
look at two areas for flash chromatography:
1. Replacing chlorinated solvents with those considered more
2. Reducing solvent use and waste generation with more
thoughtfully applied chromatography principles.
Part No: P158Issued year: 2017File size: 0.27mbFile type: pdf
As reversed-phase flash chromatography gains traction in
medicinal chemistry labs the need to monitor its cost and
safety are becoming more important. Commonly used
reversed-phase solvents typically include water with an
organic solvent such as methanol or acetonitrile – each
have advantages and disadvantages.
Part No: AN801Issued year: 2003File size: 0.27mbFile type: pdf
The heat flow parameter can be used to monitor the progress of a reaction, and can be useful
in determining reaction rates. A heat flow probe study was executed on the Advantage
Series™ 3400 process chemistry workstation for the hydrolysis of acetic anhydride in water
at temperatures from 25 to 55 °C. The observed rate constant for the hydrolysis was determined
from the heat flow measurements. An Eyring plot was produced and the enthalpy and
entropy of activation were determined. Finally, the heat of reaction was measured based on
the total amount of heat that was observed during the hydrolysis.
Part No: AN036Issued year: 2001File size: 0.55mbFile type: pdf
Activated carbon is an adsorbent media used to remove colored compounds from solution. Using this media in the Biotage FLASH-AC cartridge as a packed bed improves adsorbent performance. Increasing the temperature of the solvent modifies adsorption and alters performance of the decolorization process. Elevated temperatures increase product solubility, therefore requiring less solvent than at room temperature. The increased concentration of product in a hot solution also simplifies crystallization.
Part No: AN040Issued year: 2003File size: 0.4mbFile type: pdf
Research in CNS drugs is primarily centered on nitrogen heterocycle chemistry. Basic amines are difficult to purify using traditional silica chromatography because of strong interactions between acidic silica and the molecules’ basic amine groups. These interactions cause band spreading and poor compound recovery. Solutions employed to counteract this phenomena include adding a competing amine (e.g. triethyl amine or ammonium hydroxide) to the flash chromatography solvent system or using reversed-phase HPLC with a buffered solvent system.
Part No: P172Issued year: 2017File size: 0.49mbFile type: pdf
We investigate the use of reversed-phase high performance flash chromatography (HPFC) to quickly purify large quantities of crude synthetic peptides.
(6th Solid Phase Peptide Synthesis Symposium & 12th Australian Peptide Conference, Queensland, Australia, Oct. 2017)