Part No: PPS335Issued year: 2013File size: 0.99mbFile type: pdf
ISOLUTE PLD+ plates remove >99 % of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased sensitivity (signal-to-noise
(S/N)) for a broad range of analytes.
Part No: PN424Issued year: 2007File size: 0.15mbFile type: pdf
Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological fluid samples prior to analysis. Protein precipitation in the 96-well format, using filtration for protein removal, is a high throughput alternative to the traditional centrifugation based technique.
Part No: TN-0020.0810Issued year: 2010File size: 1mbFile type: pdf
QuEChERS (pronounced “catchers”) stands for Quick, Easy, Cheap, Effective, Rugged, and Safe. The technique is designed to be simple and involve a minimum number of steps; but still allow for effective clean up of complex samples. The QuEChERS technique involves two stages.
Part No: PPS373.V.1Issued year: 2016File size: 0.91mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to a class of strong supported acids. As a bound sulfonic acid, it has a natural propensity to bind alkali and some transition metal group metals, which means that it can be used as a scavenger for metals with a +I or +II oxidation state, such as Na, K, Li, also
Pd, Rh, and Ru.
Part No: PPS372.V.1Issued year: 2016File size: 2.4mbFile type: pdf
ISOLUTE® Si-Trisamine is a silica bound equivalent of a trisamine base and can scavenge a variety of electrophiles, including aldehydes, acid chlorides, sulfonyl chlorides, isocyanates, isothiocyanates and heavy metal ions such as Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Pb+2, Ru+2 and Zn2+.
Part No: TN0017.0406Issued year: 2006File size: 0.05mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to the class of strong acids. Similar
to ISOLUTE Si-TsOH (SCX-3)1, it can be used to (a) scavenge amines and other bases such
as anilines and borohydrides (b) as an acid catalyst in reactions or (c) as a replacement
for aqueous or organic acids in quenching reactions. The advantage of using SCX-2 as a
bound acid reagent is in that it eliminates work-up. The crude reaction mixture can be evaporated to dryness and the resulting free flowing solid can be purified directly by flash column chromatography.
Part No: TN0018.0406Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bonded equivalent of 1-propanethiol, which is useful
for covalent scavenging of alkyl, benzyl, and allyl halides as well as a variety of other
electrophiles including acid chlorides and isocyanates. In addition it can be used to
scavenge a variety of metals1 used in organic synthesis including Pd, Pt, Cu, Hg, Ag and Pb.
Part No: PPS374.V.1Issued year: 2016File size: 0.98mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bound equivalent of 1-propanethiol which is primarily used in the pharmaceutical industry as a metal scavenger for metals such as Pd, Pt, Cu, Hg, Ag and Pb but it can also be useful for covalent scavenging of alkyl, benzyl and allyl halides as well as a variety of other electrophiles including acid chlorides and isocyanates.
Part No: TN0016.0406Issued year: 2006File size: 0.07mbFile type: pdf
ISOLUTE® Si-TsOH (SCX-3) is the bound equivalent of p-toluene sulfonic acid with a
pKa <1. Similar to ISOLUTE propylsulfonic acid (SCX-2)1 , it can be used to (a) scavenge
amines and other bases such as anilines and borohydrides (b) as an acid catalyst in reactions
or (c) as a replacement for aqueous or organic acids in quenching reactions.
Part No: PPS431.v1Issued year: 2016File size: 0.48mbFile type: pdf
At Chiba University’s Graduate School of Medicine and its affiliated hospital, researchers are developing an LC-MS/MS method for vitamin D analysis using ISOLUTE® SLE+ and other Biotage products. We interviewed Professor Mamoru Satoh at the Disease Proteomics Center and Mr. Takashi Ishige from the Inspection Department at the affiliated hospital.
Part No: TN-0011.0708Issued year: 2008File size: 0.34mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction Plates offer an efficient alternative to traditional liquid-liquid extraction
(LLE) for bioanalytical sample preparation, extracting up to 400 μL of aqueous sample. ISOLUTE SLE+ plates provide
high analyte recoveries, eliminate emulsion formation, and cut sample preparation time in half.
Part No: TN-0012.0610 (2)Issued year: 2010File size: 0.35mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion
formation and reduces sample preparation time by half.
Part No: AN068Issued year: 2013File size: 0.6mbFile type: pdf
The ability to synthesize peptides in a range of different
scales is a requirement in many research laboratories. The
Biotage® Initiator+ Alstra™ fully automated microwave peptide
synthesizer has a scale range of 5 μmol up to 2 mmol using
three different reactor vial sizes (5 mL, 10 mL and 30 mL).
The acyl carrier protein fragment, ACP (65–74), H-Val-Gln-
Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is
a well-known so-called difficult sequence and is commonly
used to evaluate the performance of new synthesis reagents
Here we demonstrate a variable scale synthesis on the
Initiator+ Alstra using the ACP (65–74) fragment as a model
Part No: PPS397Issued year: 2016File size: 0.23mbFile type: pdf
Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: AN064Issued year: 2012File size: 1.09mbFile type: pdf
This application note describes the use of ISOLUTE® HM-N as a support material
for pre-adsorption of a reaction mixture prior to flash chromatography. This
approach is useful when loading reaction mixtures that are only soluble in polar
solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.
Part No: AN108Issued year: 2015File size: 0.38mbFile type: pdf
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Part No: AN109Issued year: 2015File size: 0.71mbFile type: pdf
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Part No: AN077Issued year: 2013File size: 1.35mbFile type: pdf
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Part No: P098Issued year: 2014File size: 0.28mbFile type: pdf
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.
Part No: AN085Issued year: 2014File size: 0.92mbFile type: pdf
Mass-directed flash purification is a simple and effective
technique to detect and fractionate compounds with poor UV chromophores. In this study, fatty acid methyl ester mixtures were purified by mass-directed flash purification using the Biotage Isolera™ Dalton System.
Part No: P099Issued year: 2014File size: 0.39mbFile type: pdf
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Part No: PN38Issued year: 2011File size: 0.17mbFile type: pdf
Mephedrone (MCAT) is a ‘designer drug’ synthesised to mimic the amphetamine class of compounds. Due to its perceived ‘ecstasy like’ effects of euphoria, excitement and alertness combined with ease of availability, this (until recently) unregulated drug (legal high) has found widespread abuse in the UK in recent years. Harmful effects associated with mephedrone are still being studied but after several deaths were linked to the abuse of the drug, the UK Government made this a Class B substance. Mephedrone extraction was evaluated on three mixed mode cation exchange SPE sorbents: EVOLUTE® CX, EVOLUTE® WCX and ISOLUTE® HCX.
DOA, ISOLUTE, EVOLUTE, Amphetamine
Part No: Issued year: 2011File size: 0.44mbFile type: pdf
User Report: Metal scavenger Si-Thiol. Nippon Shinyaku Co., Ltd use Biotage metal scavengers to control residual metals such as palladium, which are difficult to remove by other methods. We have been talking to Mr. Toshio Fujiwara who is in charge of Process Chemistry, CMC Research & Development Department, Discovery Research Laboratories.
Part No: P148Issued year: 2016File size: 0.93mbFile type: pdf
Biotage metal scavengers are a more effective alternative to traditional carbon batch adsorption processes. They have the lowest metals content, and also excellent extractable profiles, therefore not likely to risk contamination, unlike competitive materials we tested. Furthermore, Biotage metal scavengers offer volumetrically, a much more compact solution than carbon, requiring 48 times less volume for the same degree of Pd removal in our studies.
Part No: AN102Issued year: 2015File size: 1.12mbFile type: pdf
This study was conducted in 3 parts.
Metal scavengers were investigated for inherent metals content by ICP analysis methods.
Additionally these scavengers were extracted in a range of common solvents in order to show extractable/leachable levels.
We demonstrate an example metal scavenging mitigation step, looking at the corresponding API mass yield, purity and also metals data.
Part No: Issued year: 2013File size: 2.62mbFile type: pdf
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
» Isocratic separations are easiest to develop, but gradient separations are
» Software in the Isolera helps with conversion of an isocratic separation to
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
Part No: TN119Issued year: 2006File size: 0.11mbFile type: pdf
This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3).
Part No: TN108 rev1.3Issued year: 2004File size: 0.13mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE CBA for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchange sorbents (pages 4-6).
Part No: TN104Issued year: 2004File size: 0.06mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE NH2 for the extraction of anionic analytes from aqueous samples (pages 1-2), order information (page 3) and a general discussion on anion exchangers (pages 4-5).
Part No: TN105 rev1.3Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes), specifics on the use of ISOLUTE® PSA for the extraction of anionic
analytes from aqueous samples (page 1-2), ordering information (page 3) and a general discussion
on anion exchangers (pages 4-5). For information on extraction of anionic analytes from nonaqueous
matrices, contact Argonaut Technologies.
Part No: TN103Issued year: 2004File size: 0.07mbFile type: pdf
This technical notes includes specifics on the use of ISOLUTE SAX and PE-AX for the extraction of anionic analytes from acqueous samples (pages 1-3), ordering information (page 4) and a general discussion on anion exchangers (pages 5-6).
Part No: TN106 rev1.3Issued year: 2004File size: 0.07mbFile type: pdf
This technical note includes specifics on the use of ISOLUTE SCX and SCX-3 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchangers (pages 4-5).
Part No: TN107 rev1.4Issued year: 2004File size: 0.06mbFile type: pdf
This technical note includes, specifics on the use of ISOLUTE SCX-2 for the extraction of cationic analytes from aqueous samples (pages 1-2), ordering information (page 3) and a general discussion on cation exchanger sorbents (pages 4-5).
Part No: TN102Issued year: 2006File size: 0.11mbFile type: pdf
This Chemistry Data Sheet includes specific information on the use of polar ISOLUTE® sorbents for the extraction of polar analytes from non-aqueous samples (pages 1-2), and a general discussion on polar sorbents
Part No: P119Issued year: 2015File size: 0.45mbFile type: pdf
This poster describes the supported liquid extraction method development process for the extraction of the NBOMes group of novel psychoactive substances from oral fluid, for analysis using GC-MS. both sample pre-treatment and elution solvent were optimised, and the final method compared to traditional LLE and SPE procedures.
Part No: IST1022Issued year: 2006File size: 0.03mbFile type: pdf
Method Development Recommendations for Aflotoxins from Cereals. This represents recommendations for SPE meth
od development. The proposed steps are
based on experience with similar analytes an
d matrices, but have not been verified in
Part No: P065Issued year: 2014File size: 3.65mbFile type: pdf
This poster demonstrates method development strategies for drugs of abuse screening using supported liquid extraction in a 96-well plate and subsequent method scalability to column format to allow larger matrix volume processing prior to UPLC-MS/MS analysis.
Part No: AN726Issued year: 2011File size: 0.17mbFile type: pdf
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Part No: AN727Issued year: 2011File size: 0.11mbFile type: pdf
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Part No: AN728Issued year: 2011File size: 0.15mbFile type: pdf
Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid.
Cyclosporine, Cyclosporine, Immunosuppressant,
Part No: AN729Issued year: 2011File size: 0.18mbFile type: pdf
This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation.
Opiates, drug testing, DOA, Ion exchange
Part No: AN725Issued year: 2011File size: 0.16mbFile type: pdf
This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.
Cocaine, BZE, Benzoylecgonine, drug testing, cocaine metabolite,
Part No: P092Issued year: 2014File size: 0.36mbFile type: pdf
The fermentation process associated with the manufacture of food grade enzyme powders was recently determined to support the colonization of bacteria. To control this issue, manufactures following different international regulatory procedures have found the addition of familiar antibiotic compounds to manufacturing processes beneficial. This becomes an issue of public health as the concentration of antibiotic residues in food grade materials promotes unknown exposure to the global community. Continued interest in the biomonitoring of these compounds have inspired a number of method development strategies; however, classic methods are labor intensive and require multi-step time consuming efforts. The FDA has recently mandated a method for the determination of chloramphenicol in shellfish. This study evaluates the feasibility of method modifications to incorporate enzyme powder matrices into this method. Linearity was determined over a range of 0.1 – 5 ng/mL (r^2 > 0.990). Relative recovery was determined >80%. The typical repeatability (%RSD) for n=7 replicates <20%. Strategies to control solids prior to SPE load will be presented as they were determined critical in method performance. Method robustness was determined by evaluating multiple lots and multiple variants of enzyme powder origin. The key variable is the nature of the excipients as high level sugars of different sources need to be controlled to minimize the ESI suppression.
Part No: P154Issued year: 2017File size: 0.47mbFile type: pdf
Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize
sensitivity allowing low level analysis of vitamin B7 from human serum.
MSACL 2017, Palm Springs
Part No: Issued year: 2016File size: 2.34mbFile type: pdf
This poster describes a validated LC-MS/MS method for the quantification of nicotine, its metabolites: cotinine, trans-3’-hydroxycotinine, and the tobacco plant alkaloid anabasine in patient urine to determine their clinical utility for the evaluation of active smokers for pre-transplant eligibility.
Disclaimer: Publication of this poster in no way implies or construes endorsement of Biotage products by Emory University School of Medicine.
Part No: P146Issued year: 2016File size: 0.6mbFile type: pdf
This poster compares various sample preparation strategies for the extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of extraction techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries. Method performance was evaluated for evaporative effects, assay recovery, ion suppression, phospholipid removal and simplicity of transfer to an automated sample preparation platform.
Part No: P006Issued year: File size: 0.09mbFile type: pdf
The formation of 2,5-diketopiperazines from a water suspension of N-Boc protected dipeptide alkyl esters or dipeptide alkyl esters was induced by microwave assisted heating (Scheme 1). The products, which precipitated, were obtained in good yields. However, epimerisation/diastereomer formation was found to various extents depending on the amino acid sequence and the applied reaction temperature.
Part No: AN111Issued year: 2015File size: 1.19mbFile type: pdf
The cyclic peptide mupain-1 (CPAYSRYLDC) is an inhibitor of the cancer-related urokinase-type plasminogen activator. Here we show the synthesis of a non-natural arginine building block precursor using MAOS.
Part No: AN110Issued year: 2015File size: 0.72mbFile type: pdf
PNA oligomers have interesting properties such as their increased thermal and chemical stability and resistance to enzymatic degradation which drives their use in various molecular biology, molecular diagnostic, microarray, biosensor and antisense applications. In this study we synthesize two PNA oligomers on a small scale using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer.
Part No: Issued year: 2004File size: 0.09mbFile type: pdf
Solid-Assisted Solution Phase Synthesis (SASPS) is a technique where the target molecule is synthesized in solution and solid-supported molecules are used as reagents, catalysts or scavengers. Recently this technique has been getting a lot of attention as an alternative to solid phase organic synthesis (SPOS), since it offers many of the advantages of SPOS in terms of ease of reaction workup and product purification in addition to the advantages associated with traditional solution-phase chemistry (e.g. the ease of monitoring the progress of the reaction by simply applying LC-MS or TLC techniques).
Part No: P043Issued year: File size: 0.31mbFile type: pdf
The application of microwave irradiation in solid
phase peptide synthesis (SPPS), is an important tool
for the synthesis of difficult and long peptide
sequences that are often obtained in poor quality by
comparable conventional methods at room
temperature. Here we present the results obtained
for the synthesis of a number of difficult peptide
sequences using different levels of automation for
microwave assisted SPPS: manual, semi-automated
and fully automated systems. Syro wave, initiator, sp wave
Speeding up reactions has never been easier. Biotage microwave
synthesizers are the first-choice tools for organic chemists who
need to accelerate their production of new compounds.
Initiator, Initiator+, robot, vials, phase separator, work up tools
Part No: SS-INT.R 0611Issued year: 2011File size: 1.28mbFile type: pdf
The 60-position sample bed supports the production of focused libraries, multi-user environments and scale-out. Flexible operation enables the use of both large and small vials in combination at any time and in any order without manual intervention.
Part No: TN-VIAL.0811Issued year: 2011File size: 0.17mbFile type: pdf
Biotage® high precision microwave vials are
engineered for durability and safe reactions.
All vials are designed to withstand pressures
beyond 30 bar, and in a wide range of conditions. Technical Specification for Biotage Microwave Vials.
Part No: Issued year: File size: 0.18mbFile type: pdf
Solid-phase synthesis combined with fast and
precise heating by microwave irradiation has
become a valuable tool for peptide chemists. It
enables easier solid-phase assembly of difficult
peptide sequences and even small proteins. Here
we present, a study of microwave assisted solidphase
peptide synthesis using different resins on
the Syro WaveTM. Furthermore, we use this
methodology to synthesize difficult peptide
sequences including a multiple display
Part No: Issued year: File size: 0.45mbFile type: pdf
This paper evaluates solvent effect upon flash chromatography purification. The impact of sample mass overloading, volume overloading and sample dissolution solvents upon sample resolution are discussed.
Part No: TN0021.0707Issued year: 2007File size: 0.36mbFile type: pdf
MP-Borohydride is a macroporous, resinbound borohydride that is a resin bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones (Scheme 1). While the free form reagent is generally used in large excess and requires extraction of the reaction mixture for workup, the resin bound form can be used in slight excess and removed easily via filtration of the resin. The resin is compatible with microwave heating, which reduces the reaction time from hours to minutes4.
Part No: TN509Issued year: 2003File size: 0.12mbFile type: pdf
MP-TsO-TEMPO is a macroporous polystyrene-bound equivalent of oxoammonium p-toluenesulfonate. The
resin has primary applications in the oxidation of activated primary and secondary alcohols to their
corresponding aldehydes and ketones under mild reaction conditions.MP-TsO-TEMPO has been found to be
highly selective for oxidation of allylic, benzylic and alicyclic hydroxy compounds.Oxidations with
MP-TsO-TEMPO are very clean and the product is isolated by filtration of the resin followed by solvent removal.
Part No: TN506Issued year: 2004File size: 0.07mbFile type: pdf
MP-TsOH(65) columns have been designed specifically for isolation and purification of amines. The columns may also be used to scavenge basic reagents, substrates and reaction byproducts. A range of configurations is available from 100 mg/3 mL to 2.5 g/25 mL, including tab-less 3 and 6 mL for 48 and 24- well applications respectively. See page 6 for ordering information.
Part No: Issued year: 2005File size: 0.61mbFile type: pdf
• Outline our plans to use catalysis in synthesis and our designs on the subject utilizing small, densely-functionalized building blocks
• Discuss mechanistic issues surrounding regio- and chemoselectivity in oxidative addition with Pd in situations where a number of functional groups can be activated
• Demonstrate the concepts put to practice in natural products synthesis
• Discuss some of our catalysis work in the context of microwave-assisted organic synthesis (MAOS) and its application to library preparation
• Outline directions that we are taking with library preparation utilizing a newly designed micro flow reactor system that is driven by microwave irradiation
• Multi-component reactions (MCR) in micro channels using flow MAOS