Part No: Issued year: 2011File size: 0.72mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (“the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2013File size: 0.11mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company is mainly active within analytical and organic chemistry.
Part No: Issued year: 2011File size: 0.07mbFile type: pdf
Through innovative patented technology based on molecularly imprinted polymers (MIPs)
you will experience improved clean up in your processes. With a high probability of success,
unwanted contaminants or high value desirables can be selectively extracted from your
processes, resulting in more efficient production and cleaner products.
Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: Issued year: 2014File size: 0.12mbFile type: pdf
This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme.
MSACL EU 2014
Part No: AN057Issued year: 2012File size: 0.21mbFile type: pdf
The formation of diarylethers by reacting an arylhalide and phenol is usually a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield. The substitution patent of the electrophile and the nucleophile affects the reaction times mostly. A sterically hindered electrophile and a strongly deactivated nucleophile as outlined in the (Scheme 1) below, gives a very low yield (13 %) at conventional reflux for 2 weeks.1,2 Remainder was
recovered starting material. We have previously reported the dramatically shortened reaction time to 1 hour along with improved yield running the reaction outlined in the Scheme by heating by microwaves.
Part No: P131Issued year: 2015File size: 0.47mbFile type: pdf
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Part No: DLV_TN.0111Issued year: 2011File size: 0.08mbFile type: pdf
One of the most common flash purification challenges is
dealing with hard-to-dissolve crude samples. Because polar
solvents cause poor chromatographic results when used as
injection solvents in normal-phase flash chromatography, other
sample load options are needed.
Part No: PPS376Issued year: 2015File size: 1.6mbFile type: pdf
User Case: Kissei Pharmaceutical Co., Ltd. One of Japan’s innovative pharmaceutical companies uses Isolera™ flash
purification systems and Biotage® Initiator+ microwave synthesizers in the
development of new prescription drugs. Modern lab instruments contribute to
efficient use of time and resources at Kissei Pharmaceuticals.
Part No: P143Issued year: 2016File size: 0.27mbFile type: pdf
Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation.
This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+).
Part No: PN43Issued year: 2011File size: 0.27mbFile type: pdf
Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal.
Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011
Part No: P078Issued year: 2014File size: 0.25mbFile type: pdf
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Part No: P032Issued year: 2011File size: 1.35mbFile type: pdf
Endogenous phospholipids (outline structure shown in
Figure 1.) present in biological fluids are a major problem
in LC-MS/MS analysis. Due to their strong retention
characteristics in reversed phase chromatography
phospholipids tend not to elute as discrete peaks and are
often very difficult to separate from analytes of interest.
This co-elution often leads to areas of suppression or enhancement in the chromatogram which in turn can cause
quantitation issues. Supported liquid extraction (SLE) is an
analogous technique to traditional liquidliquid
extraction. This poster compares phospholipid removal using a wide variety of solvent combinations, pH control and polar extraction solvents on supported liquid extraction plates
Part No: Issued year: 2011File size: 1.65mbFile type: pdf
User Report: Syro I, University of Tokyo. The Suga Laboratory at RCAST, University of Tokyo, uses the Biotage Syro I automated peptide synthesizer for scaling up the chemical synthesis of non-standard peptides.
Part No: 412941-DIssued year: 2012File size: 1.18mbFile type: pdf
ELSD-1080 (evaporative light-scattering detector) is a
universal detector designed for use with Isolera One
and Isolera Four systems when purifying compounds
with little or no UV absorption such as carbohydrates,
steroids, lipids, and terpenes
Part No: AN084Issued year: 2014File size: 0.86mbFile type: pdf
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
Part No: 951.v.1.Issued year: 2010File size: 0.07mbFile type: pdf
The following describes matrix spike and surrogate spike recovery from soil samples using Biotage TurboVap II Concentration workstation. The data provided comes from a variety of actual soil samples analyzed in the laboratory. The data provided comes from a variety of actual soil samples analyzed in the laboratory.
Part No: P128Issued year: 2015File size: 0.26mbFile type: pdf
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Part No: P126Issued year: 2015File size: 0.48mbFile type: pdf
This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.
Part No: P079Issued year: 2014File size: 0.59mbFile type: pdf
The new ISOLUTE® PLD+ Protein and Phospholipid Removal Plate is highlighted in this poster. Protein and phospholipid removal, and analyte recovery are included, along with data illustrating the positive impact of clean (PL and protein free) samples in maintaining analyte signal intensity and low UPLC column back pressure over multiple LCMS runs.
Part No: P088Issued year: 2014File size: 1.06mbFile type: pdf
This poster evaluates the performance of a novel 96-well filter plate (ISOLUTE PLD+ Protein and Phospholipid Removal Plate) for
the simultaneous removal of proteins and phospholipids from serum samples prior to LC-MS/MS analysis.
Part No: P104Issued year: 2014File size: 0.77mbFile type: pdf
This poster presents the ISOLUTE PLD+ Protein and Phospholipid removal plate, highlighting its ease of use and excellent performance with respect to sample clean up, analyte recovery and elimination of back pressure build up in the UPLC system.
Part No: P153Issued year: 2016File size: 0.52mbFile type: pdf
In postmortem cases, where drugs or pesticides have been used for
their poisonous properties, traditional matrices such as urine and
whole blood may be inappropriate for qualitative and quantitative
analysis. As the site of metabolism for most drugs and toxins, the
liver may provide more insight to cause of death than other bodily
This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow.
Part No: P112Issued year: 2014File size: 1.4mbFile type: pdf
This poster demonstrates the extraction of a range of drugs of abuse from oral fluid, collected with common collection devices, prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine.
Part No: P132Issued year: 2015File size: 1.55mbFile type: pdf
This poster demonstrates the extraction of a range of drugs of abuse from oral fluid collection devices using supported liquid extraction suitable for UPLC-MS/MS analysis. Unlike some sample preparation techniques, SLE allows for the simultaneous extraction of cross-functional analytes in a single extraction protocol without forfeiting extract cleanliness.
The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine.
Part No: P087Issued year: 2014File size: 0.94mbFile type: pdf
This poster describes the extraction of a range of drugs of abuse (including barbiturates, THC and metabolites, benzodiazepines, z drugs, amphetamines,cathinones, opiates, cocaine, buprenorphine, PCP, fentanyl and ketamine) from oral fluid using supported liquid extraction (ISOLUTE SLE+) columns prior to GC-MS and LC-MS/MS analysis.
Part No: P138Issued year: 2015File size: 0.82mbFile type: pdf
This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and
Part No: P026Issued year: 2008File size: 0.36mbFile type: pdf
Matrix components, particularly salts, proteins and
phospholipids, can lead to ion suppression resulting in
inaccurate quantitation and reduced detection limits.
Resin-based mixed-mode cation exchange SPE sorbents
are widely used for the extraction of basic compounds
from biological fluids. The dual retention mechanism
allows a two stage interference wash protocol, which
results in extremely clean extracts.
This poster will investigate the performance of
EVOLUTE™ CX for the extraction of a wide range of basic
drugs from plasma, showing high analyte recovery and
advanced extract cleanliness. The analyte suite was
selected to cover a variety of basic analytes with wide
ranging polarities (LogP values). Extract cleanliness
experiments were performed showing overall ion
suppression, then individual matrix components examined
in terms of protein and phospholipid removal.
Part No: P129Issued year: 2015File size: 0.57mbFile type: pdf
Methylisothiazolinone (MI) is used in a variety of personal care products, such as sunscreens, lotions, cosmetics. MI is a cytotoxin and as a result there is concern because of sensitization and allergic reactions as well as cell and nerve damage. A percentage of the population is at risk from contact dermatitis when exposed to this compound at sufficient concentrations. This poster describes the use of ISOLUTE SLE+ for extraction of MI from sunscreen.
Part No: P151Issued year: 2016File size: 0.96mbFile type: pdf
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination.
Part No: P025Issued year: 2008File size: 0.35mbFile type: pdf
Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits.
Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic
drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined
in terms of protein and phospholipid removal.
Part No: P045Issued year: 2012File size: 0.32mbFile type: pdf
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Part No: P127Issued year: 2015File size: 0.17mbFile type: pdf
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
Part No: P064Issued year: 2014File size: 0.47mbFile type: pdf
This poster presents a simple method for the extraction and subsequent detection of both the traditional hydroxy metabolites and the biologically active dihydroxy metabolites in serum. High analyte recoveries and low ion suppression were demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites.
Part No: TN138Issued year: 2011File size: 0.5mbFile type: pdf
his technical note provides guidelines for the simultaneous extraction of acidic, basic and neutral compounds from aqueous samples or aqueous sample extracts using polymer-based non-polar SPE. The generic method is on page 1, with processing and method optimization guidelines on pages 2-3. An example application showing the trace enrichment of pharmaceuticals in water illustrates the versatility of EVOLUTE ABN for the extraction of a wide range of analytes (see Appendix) from aqueous samples.
EVOLUTE, ABN, SPE, SAMPLE PREPARATION, SOLID PHASE EXTRACTION, BASIC, NEUTRAL, ACIDIC,
Whether working in the field of bio-analysis in
drug development, clinical or forensic analysis, or
with the diversity of samples in food safety and
environmental applications, the tools and
methods used for sample preparation have a
clear impact on productivity during method
development, validation and routine analysis.
EVOLUTE SPE sorbents and extraction
methodologies have been developed to extract a
wide range of analytes while reducing or
eliminating matrix components that can cause ion
suppression in LC-MS analysis.
Part No: TN-0021.1007Issued year: 2007File size: 0.5mbFile type: pdf
EVOLUTE® ABN is an advanced water-wettable polymerbased sorbent that extracts acidic, basic and neutral analytes from biological fluids and other aqueous matrices. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE products provide a highly effective solution to the problems of ion suppression and matrix effects from dirty extracts. These high performance products allow scientists to use a generic approach for a wide range of compounds, thus reducing method development time while producing super clean extracts.
Part No: TN-0025.1010Issued year: 2010File size: 0.43mbFile type: pdf
EVOLUTE® AX mixed-mode resin-based SPE sorbent extracts a range of acidic analytes from biological fluids and other aqueous matrices using a generic procedure which minimizes method development time. EVOLUTE AX removes matrix components such as proteins, salts, nonionisable interferences and phospholipids, delivering cleaner extracts with reproducible recoveries for accurate