Part No: AN003-HORIssued year: 2009File size: 0.86mbFile type: pdf
This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly
Part No: AN002-HORIssued year: File size: 0.94mbFile type: pdf
This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.
Part No: AN022-HORIssued year: 2009File size: 0.91mbFile type: pdf
EPA Method 1657 describes the procedure to determine low ppb levels of organophosphorous pesticides in wastewater.
The disks compared in this study are: “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm).
Part No: AN008-HORIssued year: 2009File size: 0.89mbFile type: pdf
EPA Method 525.2 describes the procedure to determine
low ppb levels of semi-volatile organic material in drinking
water using solid phase extraction (SPE) or liquid-solid
extraction (LSE) techniques. The extraction solvents are
methylene chloride and ethyl acetate. Extracts are analyzed
by GC/MS using a splitless injection technique.
Part No: AN024-HORIssued year: 2012File size: 0.86mbFile type: pdf
The purpose of this application is to demonstrate the viability of a solid phase extraction (SPE) method utilizing the Horizon Technology SPE-DEX 1000/3000XL Controller with application firmware version 2.2 to extract samples for EPA Method 1664A/B and fulfill all QC requirements.
Part No: AN012-HORIssued year: 2010File size: 0.99mbFile type: pdf
The second Unregulated Contaminant Monitoring Regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 529 is categorized under List 1, Assessment Monitoring in the UCMR2 program, and focuses on three explosives: 1,3-dinitrobenzene; 2,4,6- trinitrotoluene (TNT); and hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX). The resulting data will be used by the EPA to determine whether or not regulatory standards should be established for these compounds.
Part No: AN009-HORIssued year: 2015File size: 1.33mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN019-HORIssued year: 2010File size: 0.92mbFile type: pdf
The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.
Part No: AN021-HORIssued year: 2009File size: 0.89mbFile type: pdf
Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater.
The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk.
The Biotage® Horizon 4790 Automated Extraction System with Envision®
Platform controller, and the DryVap® Automated Drying
and Concentration System were used in this application note.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: AN004-HORIssued year: 2009File size: 0.72mbFile type: pdf
This evaluation study was conducted to determine the feasibility of the Biotage SPE-DEXÒ 4790 Automated Extraction System for the extraction of herbicides and pesticides. The preliminary results demonstrate the capability of this method for the automated SPE extraction of organic compounds.
Part No: AN005-HORIssued year: 2009File size: 0.8mbFile type: pdf
EPA Method 506 is used to determine Phthalate and Adipate Esters in drinking water. The analytes are extracted from water using a Horizon Technology 47 mm Atlantic C18 disk. The disk is extracted with Acetonitrile and Methylene Chloride. The extract is then dried and concentrated to a final volume of 1.0 mL using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/PID.
Part No: AN010-HORIssued year: 2009File size: 0.8mbFile type: pdf
Method 526 is a gas chromatography / mass spectrometry(GC/MS) method for the determination of selected semivolatile compounds in raw and finished drinking waters.This method efficiently extracts analytes using the SPEDEX® 4790 with an Atlantic® 47 mm polystyrene divinylbenzene (DVB) disk. The disk is extracted with using Ethyl Acetate (EtAc) and Methylene Chloride (DCM). The extract is then dried and concentrated using the DryVap® concentrator system coupled with DryDisk® technology to a final volume of 1.0 mL. Final analysis is done by GC/MS.
Part No: AN013-HORIssued year: File size: 0.74mbFile type: pdf
EPA Method 548.1 details the procedure for the determination of endothall in drinking water by ionexchange solid phase extraction (SPE), acidic methanol methylation, and gas chromatography / mass spectrometry.
This note presents data from the State of Idaho Bureau of Labs for the initial demonstration of capability using the Biotage® Horizon T4790 Automated Extractor System.
Part No: AN015-HORIssued year: File size: 0.79mbFile type: pdf
EPA Method 552.1 is an ion exchange procedure used for determining haloacetic acids and dalapon in drinking water and drinking water sources.
The purpose of this application note is to present data demonstrating the capability of the Horizon Technology SPE-DEX® 4790 Automated Extraction System to perform the sample extraction required for this method.
Part No: AN007-HORIssued year: 2009File size: 0.64mbFile type: pdf
EPA Method 515.2 is applicable to the determination of
chlorinated acids in ground water and finished drinking
water. The form of each acid is not distinguished by this
method. Results are calculated and reported for each listed analyte as the total free acid. The analytes are extracted from the water using a DVB-Hydrophobic disk and the Horizon Technology SPE-DEXÒ 4770 Automated
Extraction System. The disk is extracted with Methylene
Chloride and the extract dried with sodium sulfate. The
extract is derivatized with diazomethane. Final analysis is by GC/ECD.
Part No: AN017-HORIssued year: 2009File size: 1.36mbFile type: pdf
The purpose of this application note is to demonstrate the use of a fully automated solid phase extraction (SPE) and concentration system that provides fast sample preparation while improving the quality and consistency of results for the Total Petroleum Hydrocarbon (TPH) compounds from C10 to C40 as dictated by EPA Method 8015.