Part No: P110Issued year: 2014File size: 0.25mbFile type: pdf
Corrosion costs the petroleum industry an estimated $1.3 billion in non-productive time, materials and labor annually. Imidazolines prepared from fatty acids and amines are a widely-used class of chemical corrosion inhibitor, due to excellent performance and ease of handling. However, commercial imidazolines are actually mixtures of several different chemical compounds, and the relative proportions of these species can have a large impact on both corrosion inhibition and product physical properties. The absence of gold standard analytical methods to characterize the active ingredients in imidazoline formulations limits the understanding of the chemistry of these materials. It is for this reason that a SPE-LC-MS method was developed to supplement the chemical information afforded by bulk testing / wet chemistry methods. It is anticipated that this method will have significant impact in the formulation of new corrosion inhibitors for oilfield applications as well as the quality control of finished products in manufacturing.
Part No: P116Issued year: 2014File size: 0.45mbFile type: pdf
This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision.
FAST LC-MS 2014
Part No: P070Issued year: 2014File size: 0.55mbFile type: pdf
This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum.
The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics.
It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis.
Part No: P052Issued year: 2013File size: 0.48mbFile type: pdf
Pain management therapy warrants constant monitoring of therapeutic levels of prescribed drug levels in patient urine samples. The number of samples being submitted for analysis has increased dramatically in the last 10 years with improvements in high throughput automated screening capabilities. Patient samples analysis is complicated by the need for an effective sample preparation methodology that can extract target analytes from complex matrices with good efficiency. Further complicating the process is the need to enzymatically hydrolyse the glucoronidated metabolites prior to extraction from the urine matrix. A fully automated sample preparation process using a TECAN Freedom EVO® 100 was designed to incorporate both the enzymatic hydrolysis and subsequent sample preparation assay as one continuous workflow. Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques was used to extract a suite of pain management drugs from spiked urine samples. A recovery and quantitation assay was run on the TECAN Freedom EVO® 100 using mock patient samples to demonstrate utility of automation process.
MSACL, Pain Management, Biotage, SPE, SLE, LLE, Supported Liquid Extraction, Drugs, MSACL, San Diego, 2013
Part No: Issued year: 2014File size: 0.88mbFile type: pdf
This poster presents the synthesis of peptidoglycan fragments where the coupling of peptides and carbohydrates was achieved using microwave heating. The fully automated total synthesis of a complex branched peptide is also presented. The fragments were synthesized using Biotage® Initiator+ SP Wave, Biotage® Syro Wave™ and Biotage® Initiator+ Alstra™ respectively. Presented at EPS, Sofia, 2014.
Part No: P047Issued year: 2012File size: 0.6mbFile type: pdf
The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread . Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields.
Cannabinoids, SLE, SLE+, Supported Liquid Extraction,
Part No: P144Issued year: 2016File size: 0.6mbFile type: pdf
The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS.
This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS.
Part No: P035Issued year: 2012File size: 0.26mbFile type: pdf
Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems and is associated with increased risk of cancers and multiple
sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. This poster presents a novel method for the extraction of 25 hydroxyvitamin D demonstrating acceptable validation parameters from serum calibrators and DEQAS supplied samples using ISOLUTE SLE+.
Part No: P109Issued year: 2014File size: 0.43mbFile type: pdf
This poster describes a supported liquid extraction method for extraction of a class of novel psychoactive substances (NBOMes) with analysis using laser diode thermal desorption (LDTD) MS/MS. Samples are oral fluid collected using Salivettes.
Part No: P081Issued year: 2014File size: 0.42mbFile type: pdf
This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated.
Part No: P091Issued year: 2014File size: 0.38mbFile type: pdf
This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers
by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance.
Part No: P044Issued year: 2012File size: 0.89mbFile type: pdf
The objective was to develop a GC-MS assay for the determination of free benzodiazepines using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods.
ISOLUTE, SLE, SLE+, Supported Liquid Extraction, Benzodiazepines, Forensic, Drugs, DOA, Drugs of Abuse, SOFT,
Part No: Issued year: 2016File size: 0.94mbFile type: pdf
Cortisol measurement in hair samples scan be a useful indicator of chronic stress. This poster compares a new LC-MS/MS method with an existing immunoassay based method.
The Biotage Bead Ruptor is used effectively for hair pre-treatment.
Part No: Issued year: 2016File size: 0.8mbFile type: pdf
Urine samples are neutralized using 0.5 M ammonium acetate and loaded onto weak cation exchange (WCX) Evolute Express plates (Biotage), preconditioned with methanol and water. Samples are washed with 20 mM ammonium acetate and water before elution in 0.5 % formic acid.
Department of Clinical Chemistry and Immunology, Heart of England NHS Foundation Trust, Birmingham, UK
Part No: P163Issued year: 2017File size: 0.58mbFile type: pdf
Over the past decade, the need for non-invasive drug screening that that precludes sample adulteration has become attractive. As a result, detection using oral fluid devices for Drugs of Abuse (DOA) has come to the vanguard of the scientific community. The use of Supported Liquid Extraction (ISOLUTE® SLE+) prior to LC/MS or GC/MS can improve sample cleanliness without forfeiting sample detection within a diverse panel of DOAs. Here, we demonstrate the effects of altering elution solvent polarity and pH for sample pretreatment upon the simultaneous recovery of 34 compounds comprised of opioids, benzodiazepines, and stimulants to directly measure the effects of the oral fluid buffer, OraSure™, upon extraction and signal intensity at presumed LOQs.
Part No: Issued year: File size: 1.03mbFile type: pdf
• Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports.
• Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling
reagents, resins, solvents etc.