Catecholamine Analysis: Method Optimization to Improve Sensitivity and Reduce Limits of Quantitation using LC-MS/MS
Part No: P137v2
Issued year: 2016
File size: 0.39mb
File type: pdf
This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine).
A number of steps are optimized to improve sensitivity of the
analytes. This includes reducing the buffer strength in the mobile
phase, reducing the buffer strength of washes, careful pH control
throughout the extraction, avoiding an evaporation step and
incorporating IPA in the reconstitution solvent.
96-well plate
Catecholamines
Clinical
Dopamine
English
Epinephrine
LC-MS/MS
Norepinephrine
Plasma
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