This is the importable .exp method for the application note AN871

3-methoxytyramine Catecholamines Dopamine Epinephrine Extrahera Metanephrine Metanephrines Norepinephrine Normetanephrine

This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma. The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.

96-well plate Catecholamines Clinical Dopamine English Epinephrine LC-MS/MS Norepinephrine Plasma Posters

This poster investigates various parts of the SPE method development process to develop a highly sensitive LC-MS/MS method for analysis of catecholamines (epinephrine, norepinephrine, dopamine). A number of steps are optimized to improve sensitivity of the analytes. This includes reducing the buffer strength in the mobile phase, reducing the buffer strength of washes, careful pH control throughout the extraction, avoiding an evaporation step and incorporating IPA in the reconstitution solvent.

96-well plate Catecholamines Clinical Dopamine English Epinephrine LC-MS/MS Norepinephrine Plasma Posters

This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.

96-well plate Application Notes Catecholamines Clinical Dopamine English Epinephrine LC-MS/MS Norepinephrine Plasma

This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from urine prior to LC-MS/MS detection. Included in this application note are the detailed settings for implementing the method on the Biotage® Extrahera(TM) automation system.

3-methoxytyramine Application Notes Catecholamines Clinical Dopamine English Epinephrine Extrahera LC-MS/MS Metanephrine Metanephrines Norepinephrine Normetanephrine Urine

This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform. MSACL 2017, Palm Springs ASMS 2017, Indianapolis

3-methoxytyramine 96-well plate Catecholamines Clinical Dopamine English Epinephrine Extrahera LC-MS/MS Metanephrine Metanephrines Norepinephrine Normetanephrine Posters Urine

This poster describes a method for the simultaneous extraction (using EVOLUTE EXPRESS WCX) and analysis of plasma catecholamines and metanephrines. A highly sensitive, linear and rugged assay was developed, and automated using the Biotage Extrahera sample preparation processing system. MSACL EU 2016

3-methoxytyramine Catecholamines Clinical Dopamine English Epinephrine Extrahera LC-MS/MS Metanephrine Metanephrines Norepinephrine Normetanephrine Plasma Posters