Part No: PPS443Issued year: 2017File size: 2.31mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: P156Issued year: 2017File size: 0.23mbFile type: pdf
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
Part No: AN790Issued year: 2013File size: 1.75mbFile type: pdf
This application note describes the extraction of different drugs in hemolyzed whole blood, which are typically screened for forensic toxicology panels, using ISOLUTE® SLE+ supported liquid extraction columns in the 1 mL and 2 mL sample capacity formats.
illicit drugs, pain management, supported liquid extraction, LC-MS/MS, blood, post mortem blood, hemolysed whole blood,
Part No: AN788Issued year: 2013File size: 1.57mbFile type: pdf
This application note describes the extraction of a range of illicit drugs, which are typically screened in forensic toxicology panels, from oral fluid using ISOLUTE® SLE+ supported liquid extraction columns.
oral fluid, Quantisal, Immunalysis, Intercept, Orasure, supported liquid extraction
Part No: P075Issued year: 2014File size: 0.66mbFile type: pdf
Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and
quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we
demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices.
Part No: AN806Issued year: 2013File size: 1.03mbFile type: pdf
The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step.
A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
Part No: AN792Issued year: 2013File size: 2.3mbFile type: pdf
This application note describes fully automated liquid handling of the extraction of a range of drugs from urine, which are typically screened for forensic toxicology panels, using ISOLUTE® SLE+ 96-well supported liquid extraction plates.
drugs of abuse, TECAN, automated, ISOLUTE SLE+
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).