Part No: PPS451Issued year: 2017File size: 2.99mbFile type: pdf
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Part No: P184Issued year: 2018File size: 0.56mbFile type: pdf
Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma.
Part No: AN887Issued year: 2017File size: 1.45mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis.
The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.
Part No: AN882Issued year: 2017File size: 1.27mbFile type: pdf
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure.
Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.
Part No: AN750Issued year: 2012File size: 1.14mbFile type: pdf
This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant.
Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,
Part No: AN869Issued year: 2016File size: 0.91mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol, using EVOLUTE EXPRESS ABN solid phase extraction plates, for the extraction of teicoplanin from plasma prior to HPLC-DAD analysis.
Part No: P049Issued year: 2012File size: 0.27mbFile type: pdf
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Part No: P162Issued year: 2017File size: 0.68mbFile type: pdf
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
Part No: AN894Issued year: 2018File size: 1.17mbFile type: pdf
This application note describes the simultaneous
extraction of aldosterone at low (< 25pg/mL) concentration and
angiotensin I and II from plasma, with the potential to improve
throughput and reduce time and costs in PRA determination.