This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated. ASMS 2014
This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance. AACC 2014
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma. The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
This poster evaluates the performance of various polymer-based ion exchange SPE chemistries for the extraction of thyroid hormones prior to LC-MS/MS analysis.
This poster compares the use of anion and cation exchange SPE approaches for the extraction of these important clinical biomarkers. MSACL Europe 2014
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX). ASMS 2017
Ciguatoxin 3C (CTX3C) Column Diarrhetic Shellfish Poisoning (DSP) Toxins Dinophysistoxin 1 (DTX1) Dinophysistoxin 2 (DTX2) English Food LC-MS/MS Marine Toxins Okadaic Acid (OA) Paralytic Shellfish Poisoning (PSP) Toxins Posters Tetrodotoxin (TTX)
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts. HPLC 2014
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches. EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
This poster provides mixed-mode resin-based SPE sample preparation strategies for the simultaneous extraction of EtG and EtS and was presented at EBF 2012. EtG, EtS, Poster, EBF, EBF 2012, Barcelona, LC-MS/MS, Ethyl Glucuronide, EVOLUTE, EVOLUTE EXPRESS, Polymeric, Polymer, SPE, Solid Phase Extraction,
EXPRESS represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combines powerful EVOLUTE sorbent chemistry with innovative features that enhance productivity by optimizing or even eliminating the need for some traditional steps in the SPE procedure. By truly eliminating the need for conditioning and equilibration, samples can be prepared by loading the plate, washing away interferences and eluting with compatible solvents
EVOLUTE® EXPRESS Solid Phase Extraction products from Biotage are designed for all your sample preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic toxicology, or with the diversity of samples in food safety and environmental applications, the tools and methods used for sample preparation have a clear impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolites (metanephrine, normetanephrine and 3-methoxytyramine) from urine prior to LC-MS/MS detection. Included in this application note are the detailed settings for implementing the method on the Biotage® Extrahera(TM) automation system.
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012. EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
ACTH (1-24) Alpha-Endorphin Angiotensin Fragment 1-7 Angiotensin II, Elafin Helicobacter pylori (2-20) LC-MS/MS Myelin Glycoprotein fragment (35-55) rat Peptides Pharma Plasma Posters Vasoactive Intestinal Fragment 1-12
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices. Pittcon 2014
96-well plate ACTH (1-24) Angiotensin Fragment 1-7 Angiotensin II, Clinical Elafin Endorphin English Glycoprotein myelin Helicobacter pylori (2-20) LC-MS/MS Peptides Pharma Plasma Posters Serum Vasoactive Intestinal Fragment 1-12
The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step. A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
6-acetyl codeine 6-acetylmorphine (MAM) 96-well plate Alprazolam Application Notes Basic Buprenorphine Clinical Clonazepam Codeine Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydromorphone LC-MS/MS Morphine Naltrexone Nitrazepam Normeperidine Oxazepam Oxycodone Oxymorphone Pain Management Temazepam Urine