The TurboVap II evaporation system concentrates organic solvent extracts using steam and nitrogen. An optical sensor allows for unattended operation, by stopping the concentration at the 1 mL endpoint. This set-up is ideal for environmental analysis. The patented vortex shearing action provides rinsing as the sample concentrates to maximize recoveries. The six position standard internal rack in the TurboVap II can be replaced with a six position rack to accommodate the new 40 mL stemmed and graduated Dionex ASE vials.
The following describes matrix spike and surrogate spike recovery from soil samples using Biotage TurboVap II Concentration workstation. The data provided comes from a variety of actual soil samples analyzed in the laboratory. The data provided comes from a variety of actual soil samples analyzed in the laboratory.
Our TurboVap II customers have been successful obtaining excellent recoveries for all semivolatile organic compounds (BNA, PAH, PCB, Pesticides, TPH/DRO, Herbicides). There are many factors that can impact the recoveries, such as client sample matrix, temperature, humidity, and extraction solvents. After gathering information from our customers, we have developed the guidelines below that will help your laboratory determine its optimum conditions for the TurboVap II.
Biotage offers the widest range of solvent evaporation systems available. This guide presents the most suitable evaporation system for your research requirements, and answers common questions regarding service, maintenance and applications. Keywords: turbovap, turbo vap, v10, v-10, spedry, spe dry, evaporation, concentrat
The TurboVap II sensor is designed to monitor the concentration process. It does this with light and logic. The sample tube stem sits in a light beam at about 0.8mL for 1mL tubes and at 0.5mL for the 0.5mL tubes. Every second, a microprocessor receives an indication of the change in optical density of the solution being concentrated. Small changes in optical density over a thirty second period are registered to memory and become the new initial or zero point for the sensor to look at the next change. When the sample is done and the meniscus crosses the sensor beam, a large change in optical density takes place and the TurboVap II beeps. The change for either dark or clear must persist for several seconds for the sensor to beep. At this point the gas flow stops and an alarm sounds to indicate that the sample is complete. When the attendant looks at the control panel, a blinking light indicates which position is complete.
In order to decide which model TurboVap will best fit a laboratory’s application, the following questions should be asked, along with following the flowchart on the reverse side: • After extraction on the Dionex ASE, what is the matrix of my extract? • After extraction on the Dionex ASE, will the extract require further cleanups or drying steps? • What other types of samples may require concentration that are not extracted on the ASE, so that I may select the model TurboVap that will be suitable for all types? • Do I have enough Dionex ASE extracts and enough non-ASE extracts to justify two separate model TurboVaps? • Does it make sense for me to purchase a standard TurboVap model now, and convert with the ASE compatible kit later?
In order to concentrate to 1.0 mL of solvent, the TurboVap must be configured such that the sensor is mounted with spacers on the bottom plate of the internal rack (Figure 1). To concentrate to 0.5 mL of solvent, the TurboVap must be configured such that the sensor is flush to the bottom plate of the internal rack (Figure 3). If the TurboVap is not correctly configured, the light beam from the sensor will not be aligned with the desired endpoint of the tube.