This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The extraction protocols were ultimately transferred to an SPE automation platform and method performance versus manual processing compared.
Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems, and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. Many sample preparation approaches to the extraction of 25-hydroxy vitamin D have been employed prior to LC-MS/MS analysis. Simple protein precipitation, supported liquid extraction and complex SPE methodology are all in routine use.
This poster was first presented at MSACL 2015, Salzburg.
Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis
Video Demonstration: Multi Racks for TurboVap®
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Video Demonstration - the Software in Turbovap® Evaporators