As the popularity of post-synthetic peptide chemistry continues to go, additional purification strategies will be required. Standard reversed phase techniques may be ineffecient due to the extreme hydrophobicity imbued by the presence of sidechain protecting groups. Herein, we demonstrate that normal phase chromatography, specifically the use of a methanol:DCM solvent system and a step gradient, can indeed facilitate rapid purification of fully protected peptides.
White Paper from Biotage: Practical Considerations Using Quantisal® Oral Fluid Collection Devices
New UV Monitor Kit for Large Scale Purification
Peptide Workflow by Biotage
Thank you for visiting our Analytica Booth
Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+