This application note details effective protocols for optimized for loading volumes of either 400 μL or 1 mL. The simple sample preparation procedure delivers clean extracts and analyte recoveries ranging from 85–99% with RSDs below 10% for all analytes.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
Cocaine, AEME, EME, Benzoylecgonine, Cocaethylene and Norcocaine.
Typical analyte % recoveries for extracted cocaine and metabolites from urine (n=7) using the described ISOLUTE® SLE+ protocol.
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution
The 2018 Analytical Catalog is Here