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Extraction of Acidic Catecholamine Metabolites in Plasma Using ISOLUTE® SLE+ Prior to LC-MS/MS Analysis

24 November 2017

This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application note achieves high reproducible recoveries (>80%, RSD < 5%) for these analytes in plasma with linearity >0.995 in the range 2–200 ng/mL.

 

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

Download the Application Note Below »

 

Analytes

  • Vanillylmandelic acid (VMA),
  • 5-hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)

 

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