Phosphatidylethanol (PEth) is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of three common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL, and can be fully automated using Biotage® Extrahera™. 96 samples can be processed in 22 minutes and 33 seconds.
ISOLUTE SLE+ supported liquid extraction fixed-well plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.
Extraction of Thyroid Hormones: T3, rT3 and T4 from Serum Using EVOLUTE® EXPRESS AX Plates Prior to LC-MS/MS Analysis
Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
Biotage Wins Five-year Peptide Patent Dispute with CEM
Are You Looking to Analyze Acrylamide in Potato Chips or Coffee – Biotage Has the Answer
Isolera™ Flash Purification System Helps This Professor Cure Disease