Phosphatidylethanol (PEth) is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of three common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL, and can be fully automated using Biotage® Extrahera™. 96 samples can be processed in 22 minutes and 33 seconds.
ISOLUTE SLE+ supported liquid extraction fixed-well plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.
Extraction of Desmopressin from Serum Using EVOLUTE® EXPRESS WCX Prior to LC-MS/MS Analysis
White Paper: Reduce Solvent Use by 50%
Perfecting Your Mass Spectrometer Parameters
Sample Preparation Strategies for Urine Panels with 50 Drugs
Biotage Synthesizer Peer-reviewed Literature