Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
EVOLUTE EXPRESS SPE products dramatically improve flow characteristics, and enhance sample preparation productivity providing clean, robust, sample preparation. This method can be automated using Biotage® Extrahera™.
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