Structural and functional studies of membrane proteins remain a challenge due to difficulties in their handling. The combination of using high concentrations of reagents (0.5M) and fast and precise microwave heating to increase the coupling efficiency using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer, enabled the efficient synthesis of peptide ‘18A’ and simplified the subsequent purification by RP-HPLC despite its relatively low solubility, to afford the desired peptide in 52% yield (468 mg). Read the full application note using the link below.
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution
The 2018 Analytical Catalog is Here