There is growing interest in naturally occurring and designed bioactive cyclic peptides. These peptides often show better stability against proteolysis than linear peptides, which can be useful in the design of peptide drugs. A new application note describes cyclic peptide synthesis using on-resin disulphide bridge formation and side-chain to side-chain cyclization. On-resin cyclization of peptides is particularly useful, and Branches™ on the Biotage® Initiator+ Alstra™ microwave peptide synthesizer makes this fast, simple and fully automated.
Branches™ provides an extensive overview for the scheduling and visualization of operations in order to simplify the programming of complex peptide modifications and is a significant addition to the peptide chemist's toolbox.
The research for this application note was carried out in the group of Prof. Knud J. Jensen at the University of Copenhagen.
The cyclic peptides synthesized via a disulfide bridge and side-chain to side-chain cyclization.
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