Mitsuhiko Kawabata is team leader of the pharmacokinetics group at Shin Nippon Biomedical. He explains:
"There are three main techniques for extracting samples - protein precipitation, liquid-liquid extraction, and solid phase extraction. Liquid-liquid extraction is falling from favor because it is cumbersome and involves a large amount of liquid. However, solid phase extraction requires many treatment steps, is cumbersome, and takes a long time, while protein precipitation, though fast, does not yield clean samples. In this sense, ISOLUTE SLE+ is an excellent option - it allows us to perform liquid-liquid extractions that yield cleaner samples and require less labor."
"It is quite simple to use. With ISOLUTE SLE+, it is only a two-step process of sample application followed by a short wait and then elution. It's really quite hassle-free, which is an enormous advantage. We had the impression that more preparation steps result in cleaner samples, So it was something of a surprise that we were able to obtain clean samples with such a simple method."
"When selecting ISOLUTE SLE+, one of our concerns was that there might be variation between lots. However, this concern turned out to be unfounded. Diatomaceous earth is simply a support for liquid-liquid partitioning, so it would not be an element that could cause a lot-to-lot difference."
Colleague Akiko Toda fills in:
"In preparing samples using liquid-liquid extraction, some of the compounds may collect in the organic solvent and water emulsion mixture, giving rise to variance in the results. With ISOLUTE SLE+, this problem does not occur. This is another reason why the analysis results have higher reproducibility."
"ISOLUTE SLE+ can be used with a sample volume as small as 200 μL. It also allows our work to be carried out very efficiently. As far as we knew, Biotage was the only manufacturer that offered a product that could satisfy this kind of need. When we use ISOLUTE SLE+, we feel the target can be extracted more efficiently and with less effort."
"Drug development takes many years, so once a method has been established, it will remain in use, sometimes as long as five or even ten years. This means that securing a stable supply that we could count on for a long time was a concern for us."
"With earlier liquid-liquid extraction, we would avoid the use of a solvent with a higher specific gravity. With SLE+ we are able to use dichloromethane. Among other things, this enables us to work with solvents we had seldom used before, so we have more trials to do."
Download the full story below.
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