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"It Was a Machine Long Awaited"

16 March 2015

Professor Hiroyuki Miyachi at Okayama University was the first user in the world to install an Isolera™ Dalton in his lab, which allowed fractionation and collection of optical isomers. “I felt like the world’s first mass-detection automated purification system had finally arrived."

 

In an interview with Professor Miyachi, he emphasizes how Isolera™ Dalton has simplified the workflow in his laboratory:

“Our work mainly involves optically active substances. Under ordinary organic synthesis reaction conditions, asymmetric compounds are normally obtained as equimolar racemic mixtures of the R and S forms. Previously, we had to analyze and identify the R and S forms separately after separation, but Isolera Dalton enables fraction collection of specific molecular weights. Simultaneously, the identity of the compound is confirmed by mass analysis and the UV absorption spectrum displayed on the monitor. In short, everything can be automated into a single system. So, we could confirm the separation of a racemate in real time and obtain several tens of milligrams of the chiral compound necessary for assay in a single operation. I consider the combination of Isolera Dalton and a medium-pressure chiral column to be its biggest practical advantage."

“The unit performs mass analysis on a tiny sample while collecting fractions on a scale of several hundred milligrams. The ingenuity involved in the fully automated sequence is truly an invention, and even works for normal phase separations. I sensed some risk for our lab as the world’s first user, but it was a machine long awaited.”

"Moreover, although MS instruments can be found at any university, they are very expensive and are frequently installed as a shared facility, and cannot be accessed on demand. In contrast, Isolera Dalton is low-priced and can be installed on the bench top for immediate use."

"We also value it as a pure MS instrument for compound verification. NMR is used to rigorously confirm the structures of the final target compounds, but rapid mass analysis for real-time determination of whether or not a synthetic intermediate was obtained during daily synthesis experiments is convenient and efficient. NMR data analysis, furthermore, requires specialized expertise, but data analysis and assurance of the correct mass can speed up our understanding of the reaction and lead to faster progress in our research.”

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Part No: Published: 2015 1.88 MB Format: pdf