The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 500 μL of serum was extracted using the EVOLUTE® EXPRESS AX 30mg fixed well plate format. High reproducible recoveries and extremely low phospholipid content were observed, demonstrating limits of quantitation of 50 pg/mL.
This is an update on the previous application for these important thyroid hormones. This improved method using EVOLUTE EXPRESS AX with multiple wash steps provides even cleaner extracts than the previous cation exchange based method and is relevant to high throughput clinical testing by LC-MS/MS.
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