ISOLUTE® FILTER+ plates provide effective, workflow friendly filtration of diverse biological samples, and are ideal for use with urine prepared by ‘dilute and shoot’ prior to UPLC-MS/MS analysis. Increased back pressures due to particulate accumulation leads to frequent downtime of UPLC systems for replacement or regeneration of the expensive analytical column. ISOLUTE FILTER+ plates are designed to prevent sample particulates from reaching the analytical UPLC column, protecting the analytical system from particulate build up over time.
ISOLUTE® FILTER+ contain a wettable 0.2 μm membrane filter for high performance particulate removal, topped by a depth filter to prevent blocking of the membrane, and can be processed using manual or automated positive pressure or vacuum manifold systems. The filtered samples will be both particulate free and visually clearer, and can be injected directly into UPLC-MS/MS systems.
Sample of whole blood following zinc sulfate precipitation and centrifugation. Left vial unfiltered; right vial filtered using ISOLUTE® FILTER+ plate.
Versatile filtration for multiple sample matrices, including
New Peptide Synthesis Workflow Brochure
Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis
New Second Generation Solvent Evaporators from Biotage
Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
How to Make up to 576 Peptides in One Run