The extraction method utilizes the EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure. Combined with the very low elution volume possible with EVOLUTE EXPRESS 10 mg formats, it eliminates the need for an evaporation step – further reducing the time needed for sample processing. LOQs of ~20pg/mL are achieved.
Catecholamines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine and norepinephrine, which are traditionally analyzed using liquid chromatography with electrochemical detection.
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Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+