The method described in this application achieves high reproducible recoveries for a number of common PFA compounds in water. The method was tested against a commonly performed DIN 38407-42 method for these compounds as an alternative extraction technique.
The Biotage method gave significantly cleaner extracts and improved extraction recoveries for the majority of the PFA suite compared to the DIN method. Extraction recoveries were consistent when loading 0.1 ng of analytes in either 2 mL or 100 mL volumes. The similarity of the extraction recoveries demonstrate that if required the sample volume could be increased to improve sensitivity without any significant deterioration in extraction recovery.
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution
The 2018 Analytical Catalog is Here