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Simultaneous Extraction of Catecholamines and Metanephrines from Plasma

29 September 2016

EVOLUTE® EXPRESS WCX 96 well plates can be used to extract catecholamines and metanephrines from pooled human plasma in a highly sensitive, linear and rugged assay. This was demonstrated in a poster presented at MSACL 2016 in Salzburg, Austria. 

 

Catecholamines and metanephrines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The presented poster describes an EVOLUTE® EXPRESS WCX method for simultaneous extraction and analysis of these important clinical analytes. The method uses a very low elution volume from the 10 mg format plates, eliminates the need for any evaporation steps, and is easily automated using Biotage® Extrahera™ sample processor.

EVOLUTE® EXPRESS combines sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning. Therefore the method was replicated without phase conditioning.

Conclusions

  • EVOLUTE® EXPRESS WCX 10 mg 96 well plates can be used to extract catecholamines and metanephrines from pooled human plasma in a highly sensitive, linear and rugged assay.
  • The addition of a water immiscible solvent wash significantly reduced the extraction recoveries of any lipid interferences.
  • Good recoveries and precision were demonstrated whether the EVOLUTE® EXPRESS WCX material was used in the standard SPE processing or modified Load-Wash-Elute protocols.
  • Features of Biotage® Extrahera™ including the high flow head and the ability to separate different washes make this an ideal instrument for performing this method.

Download the poster below