Horse urine can be notoriously viscous and challenging to work with in sample preparation. A newly developed method from Biotage demonstrates that ISOLUTE® SLE+ products handle this matrix very well, adding a new tool to combat doping in race horses.
Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses. This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis. The application highlights the use of ISOLUTE® SLE+ 48-well plate, as well as protocols for alternative plate and column based formats.
Finally - an Easy Urine Sample Preparation for LC/MS Analysis
A Smorgasbord of Analytical Methods
Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
Seminar: An Introduction to the Biotage Automated Workflow Solution
The 2018 Analytical Catalog is Here