This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
Tags: Alcohol metabolite, Application Notes, Clinical, Column, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Urine
This is a suggested emthod for the extraction of ethyl glucuronide from biological fluids (urine and serum) using a polar retention mechanism.
Tags: Application Notes
Tags: Application Notes
Using ISOLUTE NH2 SPE column.
Tags: Application Notes
Tags: Application Notes
Recomendations for SPe method development. The proposed steps are based on experience with similar analytes and matarices, but have not been verified in Biotage laboratories.
Tags: Animal feed, Application Notes
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Tags: Alcohol metabolite, Column, English, Ethyl Sulfate (EtS), Ethyl glucuronide (EtG), Forensic, LC-MS/MS, Posters, Urine
The ISOLUTE® family of anion exchange sorbents are used to extract organic anions (acidic compounds capable of exhibiting a negative charge) from both aqueous and non-aqueous matrixes.
Anion exchange SPE can be accomplished by strong (very high pKa) or weak (lower pKa) ion exchangers.
Tags: Product Notes
The ISOLUTE® family of polar sorbents are used to extract polar organic compounds from non-polar matrixes.
There are many functional groups that can exhibit polar interactions, and they have different selectivities.Some of the polar interactions that exist include dipole-dipole,dipole-induced dipole and hydrogen bonding. Polar interactions are particularly useful for the separation of molecules that are very similar in structure (e.g. structural isomers.
Tags: English, Product Notes
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...11 January 2018
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...17 July 2017
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...28 June 2017
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...15 June 2017
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...20 March 2017
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...05 January 2016
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...08 January 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...18 September 2014
ISOLUTE® NH2 solid phase extraction cartridges work well for detecting alcohol biomarkers in human urine, and a recent study reported a successful throughput of 6,000 samples per year. The key ...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
||470-0002-A||ISOLUTE® NH2 25 mg / 1mL||Consumables||100||Log in for price|
||470-0005-A||ISOLUTE® NH2 50 mg/1 mL||Consumables||100||Log in for price|
||470-0005-G||ISOLUTE® NH2 50 mg/10 mL (1 mL XL)||Consumables||50||Log in for price|
|470-0010-A||ISOLUTE® NH2 100 mg/1 mL||Consumables||100||Log in for price|
|470-0010-AG||ISOLUTE® NH2 100 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|470-0010-B||ISOLUTE® NH2 100 mg/3 mL||Consumables||50||Log in for price|
||470-0010-G||ISOLUTE® NH2 100 mg/10 mL (1 mL XL)||Consumables||50||Log in for price|
||470-0020-B||ISOLUTE® NH2 200 mg/3 mL||Consumables||50||Log in for price|
|470-0050-B||ISOLUTE® NH2 500 mg/3 mL||Consumables||50||Log in for price|
|470-0050-C||ISOLUTE® NH2 500 mg/6 mL||Consumables||30||Log in for price|
||470-0050-H||ISOLUTE® NH2 500 mg/10 mL (3 mL XL)||Consumables||50||Log in for price|
|470-0050-L||ISOLUTE® NH2 500 mg/6 mL (Glass)||Consumables||30||Log in for price|
|470-0100-C||ISOLUTE® NH2 1 g/6 mL||Consumables||30||Log in for price|
||470-0100-P01||ISOLUTE®-96 NH2 100 mg Fixed Well Plate||Consumables||1||Log in for price|
||470-0200-C||ISOLUTE® NH2 2 g/6 mL||Consumables||30||Log in for price|
|470-0200-D||ISOLUTE® NH2 2 g/15 mL||Consumables||20||Log in for price|
||9470-0100||ISOLUTE® NH2 Bulk 100 g||Consumables||1||Log in for price|