This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL.
Tags: 11-deoxycorticosterone, 11-deoxycortisol, 17-OH-Pregnenolone, 17-alpha-hydroxyprogesterone, 18-OH-Corticosterone, 21-deoxycortisol, 96-well plate, Aldosterone, Androstenedione, Application Notes, Clinical, Corticosterone, Cortisol, Cortisone, DHEA (dehydroepiandrosterone, DHEAS, Dehydroepiandrosterone (DHEA), Dihydrotestosterone (DHT), English, Estradiol, Estrogens, Estrone, LC-MS/MS, Pregnenolone, Progesterone, Serum, Steroid Hormones, Steroid metabolite
This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application achieves high reproducible recoveries and excellent linearity (>0.99) in the range 10-200 ng/mL.
Tags: 5-hydroxyindoleacetic acid (5-HIAA) , 96-well plate, Acidic catecholamine metabolites, Application Notes, Clinical, English, Homovanillic acid (HVA), LC-MS/MS, Plasma, Vanillylmandelic acid (VMA)
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure.
Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.
Tags: 96-well plate, Application Notes, Clinical, English, LC-MS/MS, Oxytocin, Peptides, Pharma, Serum, Vasopressin
The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
Tags: 96-well plate, Application Notes, Biotin, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin B7, Vitamins, Water Soluble Vitamins
This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant.
Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Application Notes, Clinical, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Plasma, Vasoactive Intestinal Fragment 1-12
This procedure is recommended for the extraction of a variety of diuretics from urine using non-polar polymeric SPE. The analyte suite includes thiazides, carbonic anhydrase inhibitors, loop and potassium sparring diuretics, with wide ranging pKa and logP values.
Biotage, polymer, resin,
Tags: Acetazolamide, Amiloride, Application Notes, Bendoflumethiazide, Bumetanide, Clinical, Column, Diuretics, Ethacrynic acid, Furosemide, Hydrochlorothiazide, Hydroflumethiazide, LC-MS/MS, Methazolamide, Spironolactone, Urine
This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency.
Pharmaceuticals, waste water, antibiotics, EVOLUTE,
Tags: Application Notes, Atenolol, Carbamazepine, Citalopram, Column, Diclofenac, Environmental, Erythromycin, Fluoxetine, Fluvoxamine, HPLC, Ibuprofen, Labetalol, Mefenamic, Metoprolol, Oxprenolol, Paroxetine, Pharmaceuticals, Propranolol, Sotalol, Sulfamethoxazole, Trimethoprim, Water
Customer Case: Japan Frozen Foods Inspection Corporation.
The Japan Frozen Foods Inspection Corporation (JFFIC) made the transition from traditional mouse
testing of shellfish toxins, to instrumental analysis by developing original methods. In the sample
preparation procedure JFFIC uses EVOLUTE® ABN hydrophilic/hydrophobic SPE columns from Biotage.
Tags: Customer Cases
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides
This poster compares sample preparation options (solid phase extraction using EVOLUTE EXPRESS ABN, and supported liquid extraction using ISOLUTE SLE+) for the extraction of a panel of endogenous steroids from serum.
MSACL NA 2018, Palm Springs, CA
Tags: 96-well plate, Clinical, English, LC-MS/MS, Posters, Serum, Steroid Hormones, Steroid metabolite
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
Tags: 96-well plate, Clinical, English, Oxytocin, Peptides, Pharma, Posters, Serum, Vasopressin
Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize
sensitivity allowing low level analysis of vitamin B7 from human serum.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Tags: 96-well plate, Biotin, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin B7, Vitamins, Water Soluble Vitamins
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
Tags: 96-well plate, Amikacin, Aminoglycoside antibiotics, Apramycin, Dihydrostreptomycin, Eggs, English, Extrahera, Food, Gentamicin, Gentamicin C1, Gentamicin C1a, Gentamicin C2, Hygromycin B, Kanamycin, LC-MS/MS, Meat, Milk, Neomycin, Netilmicin, Paromomycin, Posters, Sisomycin, Spectinomycin, Streptomycin, Tobramycin, Veterinary drugs
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX).
Tags: Ciguatoxin 3C (CTX3C), Column, Diarrhetic Shellfish Poisoning (DSP) Toxins, Dinophysistoxin 1 (DTX1), Dinophysistoxin 2 (DTX2), English, Food, LC-MS/MS, Marine Toxins, Okadaic Acid (OA), Paralytic Shellfish Poisoning (PSP) Toxins, Posters, Tetrodotoxin (TTX)
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Tags: 96-well plate, Catecholamines, Clinical, Dopamine, English, Epinephrine, LC-MS/MS, Norepinephrine, Plasma, Posters
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
Tags: Acidic, Amitriptyline, Atenolol, Benzenesulfonic acid, Bretylium, Clinical, Column, Diethyldithiophosphate (DEDTP), Dimethylthiophosphate (DMTP), English, Extrahera, Forensic, Hexanesulfonic acid, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Organophosphate Pesticide, Over the counter pharmaceuticals, Pain Management, Pharma, Pharmaceuticals, Posters, Prednisolone, Procainamide, Quaternary Amines, Quinidine, Salbutamol, Sulindac, Tricyclic Antidepressant, Urine, β-blockers
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12
Naturally occurring corticosteroids are steroid hormones produced in the adrenal cortex and perform many functions within the body. Due to their widespread action
many synthetic corticosteroids based on the hydrocortisone (cortisol) structure have been produced. Some of their uses involve treatment of various forms of arthritis, dermatitis, hepatitis and ulcerative colitis. They can be administered orally, injected into veins or muscle, nasal sprays and drops or used as topical creams. Reliable
screening methods for these drugs are important as they are classed as ‘banned substances’ by many anti-doping agencies and regulated for use in meat producing animals.
Four posters and a seminar on sample preparation developments from Biotage were presented at MSACL, Palm Springs in January, now made available to the public. Posters Extraction...31 January 2018
This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) fro...08 November 2017
This compendium highlights 43 clinical sample preparation applications from Biotage. Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for e...24 October 2017
This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...02 October 2017
Many toxicology labs are moving to larger drug panels to increase throughput and reduce turn-around-time and cost. Methods using LC-MS with 50 or more drugs and metabolites are increasingly common,...05 July 2017
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...29 May 2017
Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...19 May 2017
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...20 April 2017
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...14 March 2017
A White Paper from Biotage Abstract Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues f...22 February 2017
Four posters were presented at MSACL in Palm Springs, CA, 22-26 January 2017. P154: Method Optimization for the Low Level Detection of Vitamin B7 from Human Serum Using UPLC-MS/MS An...10 February 2017
The method described in this application from Biotage Labs demonstrates high, reproducible recovery of teicoplanin from human plasma. Samples were extracted using an EVOLUTE® EXPRESS ABN 30 mg ...06 December 2016
Could there be a sorbent for versatile sample preparation that doesn't need conditioning and equilibration? Actually, Biotage has developed five such sorbents in the EVOLUTE® EXPRESS family, an...06 October 2016
The Japan Frozen Foods Inspection Corporation (JFFIC) made the transition from traditional mouse testing of shellfish toxins, to instrumental analysis by developing original methods. In the sample ...15 October 2015
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...13 March 2015
In a study aimed at determining the pharmacokinetic characteristics and metabolism of eprinomectin in cats, EVOLUTE® ABN plates were used to quantify eprinomectin B1a in plasma. A vali...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...10 July 2014
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...30 June 2014
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/20 May 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...01 April 2014
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...11 January 2014
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...28 August 2012
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...28 August 2012
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...18 April 2011
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...16 December 2010
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...23 February 2010
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...04 January 2008
[2008-01-04] Biotage to Unveil New Advanced Sample Preparation Products at PITTCON 2008 Biotage, the leading supplier of tools and technology for medical and analytical chemistry, announce...
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|600-0001-AXG||EVOLUTE® EXPRESS ABN 10 mg/1 mL (Tabless)||Consumables||100||Log in for price|
|600-0003-AXG||EVOLUTE® EXPRESS ABN 30 mg/1 mL (Tabless)||Consumables||100||Log in for price|
||600-0010-PX01||EVOLUTE® EXPRESS ABN 10 mg Fixed Well Plate||Consumables||1||Log in for price|
|600-0030-PX01||EVOLUTE® EXPRESS ABN 30 mg Fixed Well Plate||Consumables||1||Log in for price|
|610-0006-BXG||EVOLUTE® EXPRESS ABN 60 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|610-0010-BXG||EVOLUTE® EXPRESS ABN 100 mg/3 mL (Tabless)||Consumables||50||Log in for price|
|610-0015-CXG||EVOLUTE® EXPRESS ABN 150 mg/6 mL (Tabless)||Consumables||30||Log in for price|
|610-0050-CXG||EVOLUTE® EXPRESS ABN 500 mg/6 mL (Tabless)||Consumables||30||Log in for price|
||650-0010-PX01||EVOLUTE® EXPRESS SORBENT SELECTION 10 mg Fixed Well Plate||Consumables||1||Log in for price|
||650-0030-PX01||EVOLUTE® EXPRESS SORBENT SELECTION 30 mg Fixed Well Plate||Consumables||1||Log in for price|
|Part No.||Description||Product type||Pack Size||Price|
|121-9620||Tabless 1 mL Column Holder, VacMaster-96||Accessories||1||Log in for price|
|PPM-A96-CH||1 mL (Tabless) Column Holder, PRESSURE+ 96||Accessories||1||Log in for price|